CAJ | 학술논문

背景:粒细胞集落刺激因子对成纤维细胞、角质细胞、皮肤黏膜细胞均有不同程度的刺激作用.目的:观察粒细胞集落刺激因子对药物性外渗所致皮肤溃疡的愈合作用.设计:随机对照动物实验.单位:解放军第四军医大学西京医院呼吸内科.材料:实验于2004-06/2004-11在解放军第四军医大学西京医院呼吸内科实验室完成.取雄性昆明种小白鼠20只,体质量18～24 g.方法:将制备好的皮肤溃疡动物模型随机分为对照组和治疗组,每组10只.对照组给予酚妥拉明1 mg,利多卡因20 mg,地塞米松1 mg,用生理盐水稀释至0.5 mL,封闭,1次/d,共7 d;治疗组在溃疡周围注射粒细胞集落刺激因子25 μg,用生理盐水稀释至0.5 mL,隔日给药1次,共7 d.观察溃疡处皮肤组织的愈合时间和组织学变化.主要观察指标:观察粒细胞集落刺激因子对药物性外渗所致皮肤溃疡、糜烂的愈合时间和组织学变化.结果:20只小白鼠均进入结果分析.①愈合时间:对照组糜烂、溃疡的愈合时间明显长于治疗组[(20～24,8～12)d,t=2.264,P=0.01];②组织学观察:对照组治疗7 d后肉芽组织增生不明显;治疗组治疗7 d后肉芽组织增生,新生血管丰富.结论:粒细胞集落刺激因子能促进药物性糜烂、溃疡的创伤愈合.
배경:립세포집락자격인자대성섬유세포、각질세포、피부점막세포균유불동정도적자격작용.목적:관찰립세포집락자격인자대약물성외삼소치피부궤양적유합작용.설계:수궤대조동물실험.단위:해방군제사군의대학서경의원호흡내과.재료:실험우2004-06/2004-11재해방군제사군의대학서경의원호흡내과실험실완성.취웅성곤명충소백서20지,체질량18～24 g.방법:장제비호적피부궤양동물모형수궤분위대조조화치료조,매조10지.대조조급여분타랍명1 mg,리다잡인20 mg,지새미송1 mg,용생리염수희석지0.5 mL,봉폐,1차/d,공7 d;치료조재궤양주위주사립세포집락자격인자25 μg,용생리염수희석지0.5 mL,격일급약1차,공7 d.관찰궤양처피부조직적유합시간화조직학변화.주요관찰지표:관찰립세포집락자격인자대약물성외삼소치피부궤양、미란적유합시간화조직학변화.결과:20지소백서균진입결과분석.①유합시간:대조조미란、궤양적유합시간명현장우치료조[(20～24,8～12)d,t=2.264,P=0.01];②조직학관찰:대조조치료7 d후육아조직증생불명현;치료조치료7 d후육아조직증생,신생혈관봉부.결론:립세포집락자격인자능촉진약물성미란、궤양적창상유합.
BACKGROUND: Recombinant human granulocyte-macrophage colony stimulating factor (rhGM-CSF) could stimulate the proliferation of fibroblasts, keratinocyte and skin mucosae cells to different degrees.OBJECTIVE: To observe the effect of rhGM-CSF on the healing of drug exosmose induced skin ulcers.DESIGN: A randomized and controlled animal experiment.SETTING: Department of Respiratory Medicine, Xijing Hospital, Fourth Military Medical University of Chinese PLA.MATERIALS: This experiment was carried out at the Department of Respiratory Medicine, Xijing Hospital, Fourth Military Medical University of Chinese PLA from June to November 2004. Totally 20 male Kunming white mice, with body mass of 18 to 24 g, were chosen.METHODS: Prepared skin ulcers animal models were randomly divided into control group and treated group with 10 white mice in each group.Mice in the control group were given 1mg phentolamine ,20 mg lidocaine and 1mg dexamethasone diluted by normal saline to 0.5 mL ,then sealed up , once a day for 7 days; 25 μg rhGM-CSF was diluted by normal saline to 0.5 mL , then the solution was injected into the periphery of ulcers of mice in treated group , once every other day, for 7 days. Healing time and histological change of skin tissue at ulcer were observed.MAIN OUTCOME MEASURES: To observe the effect of rhGM-CSF on the healing time of drug exosmose induced skin ulcer and anabrosis and histological changeRESULTS: Totally 20 mice entered the stage of result analysis. ①Healing time: the healing time of ulcer and erosion was significantly longer in control group than in treated group [(20-24,8-12)d,t=2.264,P=0.01];②Histological observation: hyperplasia of granulation tissue was not obviously on 7 days after treatment in control group; Hyperplasia of granulation tissue appeared and the newly born blood vessel was abundant on 7 days after treatment in the treated group.CONCLUSION: rhGM-CSF can promote the wound healing of drug induced anabrosis and ulcer.