CAJ | 학술논문

本研究探讨干扰素α对慢性髓系白血病(CML)来源的树突状细胞(DC)表达Fas/FasL的影响.在CML-DCs的培养液中除加入SCF,GM-CSF,TNF-α及IL-4外,还加入IFN-α.培养10-14天,除了鉴定细胞免疫表型和Ph1染色体比例外,还应用流式细胞仪检测细胞表达Fas/FasL比例,用PI染色分析细胞凋亡,ELISA法检测上清液sFas含量.结果表明:加入IFN-α后,CML-DC共刺激分子的表达显著改善,Ph1(+)细胞比例随IFN-α浓度增加而减低;培养细胞Fas的表达上调,sFas含量却下降,FasL表达阴性,细胞凋亡比例增加.结论:IFN-α在改善CML-DC表型同时,可通过Fas途径促进Ph1(+)细胞凋亡,使Ph1'(-)细胞数量相对增加.
본연구탐토간우소α대만성수계백혈병(CML)래원적수돌상세포(DC)표체Fas/FasL적영향.재CML-DCs적배양액중제가입SCF,GM-CSF,TNF-α급IL-4외,환가입IFN-α.배양10-14천,제료감정세포면역표형화Ph1염색체비예외,환응용류식세포의검측세포표체Fas/FasL비례,용PI염색분석세포조망,ELISA법검측상청액sFas함량.결과표명:가입IFN-α후,CML-DC공자격분자적표체현저개선,Ph1(+)세포비례수IFN-α농도증가이감저;배양세포Fas적표체상조,sFas함량각하강,FasL표체음성,세포조망비례증가.결론:IFN-α재개선CML-DC표형동시,가통과Fas도경촉진Ph1(+)세포조망,사Ph1'(-)세포수량상대증가.
The study was aimed to investigate the influence of interferon α (IFN-α) on the expressions of Fas and Fas ligand (FasL) in dendritic cells (DCs) from patients with chronic myeloid leukemia (CML). In addition to adding stem cell factor(SCF), granulocyte-macrophage colony-stimulating factor (GM-CSF), tumor necrosis factor α (TNF-α) and interleukin 4 (IL-4), the IFN-α was added to the serum-free medium for DCs. After culturing for 10-14 days,cell phenotype and percentage of Ph1 chromosome were detected by different methods. The expression of Fas or FasL on CML-DCs and cell cycle of DCs labeled with propidium iodine (PI) were measured by flow cytometry. The concentra-tion of sFas in supernatants was analyzed by enzyme-linked immunosorbent assay (ELISA). The results indicated thatthe expression of co-stimulatory molecules were improved significantly while the percentages of Ph1 positive cells de-creased. The level of Fas on cells was up-regulated and the concentration of sFas decreased. However, the expression of FasL was negative. The ratio of apoptosis rose gradually while the concentration of IFN-α increased. It is concluded that IFN-α can accelerate the apoptosis of Ph1 positive cells through Fas/FasL pathway, so the number of Ph1 negative cells increases relatively.