中国临床神经外科杂志
中國臨床神經外科雜誌
중국림상신경외과잡지
CHINESE JOURNAL OF CLINICAL NEUROSURGERY
2009年
7期
418-421
,共4页
孟亮%陈治标%张百芳%戴小琴%许明涛%叶应湖
孟亮%陳治標%張百芳%戴小琴%許明濤%葉應湖
맹량%진치표%장백방%대소금%허명도%협응호
脑挫裂伤%甘露醇%尼莫地平%水通道蛋白-4%创伤性脑水肿
腦挫裂傷%甘露醇%尼莫地平%水通道蛋白-4%創傷性腦水腫
뇌좌렬상%감로순%니막지평%수통도단백-4%창상성뇌수종
Traumatic brain injury%Mannitol%Nimodipine%Aquaporin-4%Brain edema
目的 观察甘露醇与尼莫地平联合应用对大鼠挫裂伤脑组织水通道蛋白-4(AQP-4)表达的影响.方法 成年Wistar大鼠54只,随机分为挫裂伤组、甘露醇治疗组、甘露醇与尼莫地平联合治疗组.采用文献报告的方法制作大鼠左顶叶皮层局限性脑挫裂伤模型.各组分别于脑挫裂伤后24、48、72 h取挫伤区脑组织,检测其含水量以及AQP-4的表达.结果 随着伤后时间的增加,各组脑组织含水量增加,AQP-4mRNA和AQP-4蛋白的表达均逐渐增高(P<0.05);与挫裂伤组相比较,甘露醇治疗组伤后各时间点脑组织含水量、AQP-4mRNA和AQP-4蛋白的表达均明显增加(P<0.05);甘露醇与尼莫地平联合治疗组伤后各时间点与前两组相比,脑组织含水量及AQP-4 mRNA和AQP-4蛋白的表达均明显降低(P<0.05).结论 脑挫裂伤后,AQP-4 mRNA和AQP-4蛋白的表达增加;脑组织含水增加,甘露醇联合应用尼莫地平后,能够下调AQP-4 mRNA和AQP-4蛋白的表达,有效缓解创伤性脑水肿.
目的 觀察甘露醇與尼莫地平聯閤應用對大鼠挫裂傷腦組織水通道蛋白-4(AQP-4)錶達的影響.方法 成年Wistar大鼠54隻,隨機分為挫裂傷組、甘露醇治療組、甘露醇與尼莫地平聯閤治療組.採用文獻報告的方法製作大鼠左頂葉皮層跼限性腦挫裂傷模型.各組分彆于腦挫裂傷後24、48、72 h取挫傷區腦組織,檢測其含水量以及AQP-4的錶達.結果 隨著傷後時間的增加,各組腦組織含水量增加,AQP-4mRNA和AQP-4蛋白的錶達均逐漸增高(P<0.05);與挫裂傷組相比較,甘露醇治療組傷後各時間點腦組織含水量、AQP-4mRNA和AQP-4蛋白的錶達均明顯增加(P<0.05);甘露醇與尼莫地平聯閤治療組傷後各時間點與前兩組相比,腦組織含水量及AQP-4 mRNA和AQP-4蛋白的錶達均明顯降低(P<0.05).結論 腦挫裂傷後,AQP-4 mRNA和AQP-4蛋白的錶達增加;腦組織含水增加,甘露醇聯閤應用尼莫地平後,能夠下調AQP-4 mRNA和AQP-4蛋白的錶達,有效緩解創傷性腦水腫.
목적 관찰감로순여니막지평연합응용대대서좌렬상뇌조직수통도단백-4(AQP-4)표체적영향.방법 성년Wistar대서54지,수궤분위좌렬상조、감로순치료조、감로순여니막지평연합치료조.채용문헌보고적방법제작대서좌정협피층국한성뇌좌렬상모형.각조분별우뇌좌렬상후24、48、72 h취좌상구뇌조직,검측기함수량이급AQP-4적표체.결과 수착상후시간적증가,각조뇌조직함수량증가,AQP-4mRNA화AQP-4단백적표체균축점증고(P<0.05);여좌렬상조상비교,감로순치료조상후각시간점뇌조직함수량、AQP-4mRNA화AQP-4단백적표체균명현증가(P<0.05);감로순여니막지평연합치료조상후각시간점여전량조상비,뇌조직함수량급AQP-4 mRNA화AQP-4단백적표체균명현강저(P<0.05).결론 뇌좌렬상후,AQP-4 mRNA화AQP-4단백적표체증가;뇌조직함수증가,감로순연합응용니막지평후,능구하조AQP-4 mRNA화AQP-4단백적표체,유효완해창상성뇌수종.
Objective To observe the effect of mannitol and nimodipine on traumatic brain edema and aquaporin-4 (AQP-4) expression. Methods Fifty-four adult Wistar rats, who suffered from traumatic brain injury(TBI) of the left parietal lobe, were randomly divided into three groups, i.e. blank (no treatment) group (group A), treatment group with mannitol (group B), and treatment group with mannitol and nimodipine (group C). The water content and expression of AQP-4 were determined in the confused brain tissues 24, 48 and 72 hours after TB1 in all the rats. Results The brain water content, and expressions of AQP--4 mRNA and AQP-4 protein gradually increased in all the groups with the lapse of time after TBI (P<0.05). The brain water content, and expressions of AQP-4 mRNA and AQP-4 protein were significantly higher in group B than those in group A after TBI (P<0.05). The brain water content, and expressions of AQP-4 mRNA and AQP-4 protein were significantly lower in group C than those in group A and B after TBI (P<0.05). Conclusions The expressions of AQP-4 mRNA and AQP-4 protein, and the water content increase in the contused brain tissues after TBI. Administration of mannitol combined with nimodipine can inhibit the up-regulation of the expression of AQP-4 mRNA and AQP-4 protein, and can effectively relieve the brain edema in the rats with TBI.
