CAJ | 학술논문

目的:研究栀子苷、黄芩苷和小檗碱对缺氧/复氧损伤时大鼠脑微血管内皮细胞的保护机制.方法:建立离体大鼠脑微血管内皮细胞缺氧4 h复氧12 h损伤模型.用0.128,0.064,0.032 μmol·mL-1栀子苷,0.028,0.014,0.007 μmol.mL-1黄芩苷和0.024,0.012,0.006 μmol.mL-1小檗碱分别作用于损伤的细胞,采用免疫细胞化学方法和图象定量分析技术检测核因子-κB(NF-κB)亚单位p65的表达,计算平均吸光度和平均面积,比较NF-κB蛋白表达的变化,计算核移位百分率和细胞核与细胞浆的透光度比值比较NF-κB核移位的变化.结果:模型组NF-κB蛋白表达和核移位显著高于正常组(P＜0.01).所有给药组的平均吸光度值低于模型组,但差异无统计学意义.所有给药组的平均面积均低于模型组(P＜0.01).所有给药组的核移位百分率低于模型组且高于正常组(P＜0.01,P＜0.01).所有给药组细胞核与细胞浆的透光度比值高于模型组(P＜0.01).结论:栀子苷、黄芩苷和小檗碱等黄连解毒汤有效成分能够保护缺氧/复氧损伤的大鼠脑微血管内皮细胞,其保护机制之一是抑制NF-κB蛋白表达和核移位.
목적:연구치자감、황금감화소벽감대결양/복양손상시대서뇌미혈관내피세포적보호궤제.방법:건립리체대서뇌미혈관내피세포결양4 h복양12 h손상모형.용0.128,0.064,0.032 μmol·mL-1치자감,0.028,0.014,0.007 μmol.mL-1황금감화0.024,0.012,0.006 μmol.mL-1소벽감분별작용우손상적세포,채용면역세포화학방법화도상정량분석기술검측핵인자-κB(NF-κB)아단위p65적표체,계산평균흡광도화평균면적,비교NF-κB단백표체적변화,계산핵이위백분솔화세포핵여세포장적투광도비치비교NF-κB핵이위적변화.결과:모형조NF-κB단백표체화핵이위현저고우정상조(P＜0.01).소유급약조적평균흡광도치저우모형조,단차이무통계학의의.소유급약조적평균면적균저우모형조(P＜0.01).소유급약조적핵이위백분솔저우모형조차고우정상조(P＜0.01,P＜0.01).소유급약조세포핵여세포장적투광도비치고우모형조(P＜0.01).결론:치자감、황금감화소벽감등황련해독탕유효성분능구보호결양/복양손상적대서뇌미혈관내피세포,기보호궤제지일시억제NF-κB단백표체화핵이위.