CAJ | 학술논문

　　在金电极表面电沉积二氧化锆纳米粒子以固定乙酰胆碱酯酶（AChE），制成AChE/ZrO2粒子层/金电极（AChE/ZrO2/Au）电流型有机磷生物传感器，可用于液相中有机磷农残的快速检测。以对氧磷为分析目标物，研究了传感器检测茶叶有机磷农残的主要响应性能。结果表明，该传感器在5.0×10-7～5.5×10-4 g/L浓度范围内对目标分析物有线性响应，检出限为1.0×10-7 g/L。该传感器构造简单，检测灵敏度高、非特异性吸附小、再生性好。
　　재금전겁표면전침적이양화고납미입자이고정을선담감지매（AChE），제성AChE/ZrO2입자층/금전겁（AChE/ZrO2/Au）전류형유궤린생물전감기，가용우액상중유궤린농잔적쾌속검측。이대양린위분석목표물，연구료전감기검측다협유궤린농잔적주요향응성능。결과표명，해전감기재5.0×10-7～5.5×10-4 g/L농도범위내대목표분석물유선성향응，검출한위1.0×10-7 g/L。해전감기구조간단，검측령민도고、비특이성흡부소、재생성호。
In this paper, a novel biosensor for detecting organophosphorus has been prepared. First, zirconium dioxide was electrodeposited onto the cleaned surface of gold electrode. Second, acetylcholinesterase was immobilized on these zirconium nanoparticles by adsorption. Then, the concentration of organophosphorus solution could be determined after putting the electrode into organophosphorus solution at different concentrations. Taking paraoxon as an example, the response character of the biosensor has been investigated. The proposed biosensor in optimal conditions has a linear range of 5.0×10-7~5.5×10-4 g/L for paraoxon detection, and its detection limit is 1.0×10-7 g/L. The process for preparation of the biosensor is simple and convenient. Moreover, the proposed biosensor has advantages in high sensitivity, low non-specific absorption and regenerability.