CAJ | 학술논문

乙烯受体基因ETR1是乙烯信号转导过程中的关键调控基因.研究根据ETR1基因的保守序列设计引物,以西瓜(Citrullus lanatus (Thunb.) Matsum & Nadai var.lanatus)和黄瓜(Cucumis sativus L.)的基因组DNA为模板进行PCR扩增,获得序列长度分别为1 633 bp和1 491 bp的基因片段CLETR1和CSETR1.序列分析表明,CLETR1和CSETR1与Genebank中收录的多条ETR1基因的核苷酸序列同源性在80%～98%,氨基酸序列同源性在75%～98%.西瓜和黄瓜ETR1基因片段的编码序列存在明显的单核苷酸变异,共23个核苷酸位点存在SNPs(Single nucleotide polymorphisms),其中5个SNPs导致4个编码氨基酸的改变.
을희수체기인ETR1시을희신호전도과정중적관건조공기인.연구근거ETR1기인적보수서렬설계인물,이서과(Citrullus lanatus (Thunb.) Matsum & Nadai var.lanatus)화황과(Cucumis sativus L.)적기인조DNA위모판진행PCR확증,획득서렬장도분별위1 633 bp화1 491 bp적기인편단CLETR1화CSETR1.서렬분석표명,CLETR1화CSETR1여Genebank중수록적다조ETR1기인적핵감산서렬동원성재80%～98%,안기산서렬동원성재75%～98%.서과화황과ETR1기인편단적편마서렬존재명현적단핵감산변이,공23개핵감산위점존재SNPs(Single nucleotide polymorphisms),기중5개SNPs도치4개편마안기산적개변.
ETR1 was the controlling gene in ethylene signal transduction. A pair of oligo nucleotide primers were de-signed from conserved domain of ETR1 gene family. PCR amplifications were performed on genomic DNA template of Cit-rullus lanatus (Thunb. ) Matsum & Nadai var. lanatus and Cucumis sativus L., they produced two fragments of 1 633 bp and 1 491 bp,named CLETR1 and CSETR1 respectively.The results of Blastn on NCBI Genebank database indicated that many highly matched homologous nucleic acid sequences and amino acid sequences were all ethylene receptor gene, the ratio were 80% -95% and 75% -90% respectively. The single nucleotide variations were found in the conserved sequences between CLETR1 and CSETR1,there were 23 SNPs(single nucleotide polymorphisms) in the encoding region, and 5 of them resulted 4 amino acids difference.