CAJ | 학술논문

目的:研究泰山赤灵芝多糖处理动脉粥样硬化大鼠前后血脂的变化和胸主动脉细胞差异表达的基因,了解泰山赤灵芝多糖治疗动脉粥样硬化的分子机制.方法: 全自动生化分析仪测定泰山赤灵芝多糖处理动脉粥样硬化大鼠前后血清TG、TC、HDL-C含量的变化;通过差异显示逆转录PCR(DDRT-PCR)技术研究泰山赤灵芝多糖处理动脉粥样硬化大鼠前后胸主动脉细胞的基因表达差异,并用反向Northern证实差异显示基因片段.结果: 泰山赤灵芝多糖处理动脉粥样硬化大鼠后,TG、TC含量均明显下降,而HDL-C和HDL-C/TC百分比明显升高;胸主动脉细胞出现一些表达上调和下调的基因片段.上调基因有细胞色素B561、ERP72、nitric oxide synthase 2和Gpx1;下调基因有胸腺素β4、FGFRI原癌基因伴随蛋白、FK506结合蛋白、ICAM-1、c-myc和TNF-α.结论: 泰山赤灵芝多糖可减轻动脉粥样硬化风险,降低血脂.
목적:연구태산적령지다당처리동맥죽양경화대서전후혈지적변화화흉주동맥세포차이표체적기인,료해태산적령지다당치료동맥죽양경화적분자궤제.방법: 전자동생화분석의측정태산적령지다당처리동맥죽양경화대서전후혈청TG、TC、HDL-C함량적변화;통과차이현시역전록PCR(DDRT-PCR)기술연구태산적령지다당처리동맥죽양경화대서전후흉주동맥세포적기인표체차이,병용반향Northern증실차이현시기인편단.결과: 태산적령지다당처리동맥죽양경화대서후,TG、TC함량균명현하강,이HDL-C화HDL-C/TC백분비명현승고;흉주동맥세포출현일사표체상조화하조적기인편단.상조기인유세포색소B561、ERP72、nitric oxide synthase 2화Gpx1;하조기인유흉선소β4、FGFRI원암기인반수단백、FK506결합단백、ICAM-1、c-myc화TNF-α.결론: 태산적령지다당가감경동맥죽양경화풍험,강저혈지.
AIM: To study the change of blood lipid and difference of expressed gene in chest aorta cells of atherosclerosis model rats with or without the treatment of Taishan Ganoderma Lucidum amylose.METHODS: The concentrations of triglyceride (TG), total cholesterol (TC) and high-density lipoprotein cholesterol (HDL-C) in serum were measured by automatic serum biochemical assay. Differential-display reverse transcription-PCR (DDRT-PCR) was used to analyze the differential expressed gene in chest aorta cells of atherosclerosis model rat with or without the treatment of Taishan Ganoderma Lucidum amylose. The differential expressed cDNA was confirmed by reverse Northern blotting.RESULTS: Up-regulated and down-regulated cDNA were isolated in chest aorta cells of atherosclerosis model rats with or without Taishan Ganoderma Lucidum amylose treatment. The known up-regulated genes included cytB561, ERP72, nitric oxide synthase 2 and Gpx1. The known down-regulated genes included thymosin β4, FGFRI oncogene, ICAM-1, FK506 binding protein, c-myc and TNF-α. CONCLUSION: Taishan Ganoderma Lucidum amylose decreases the level of blood lipid.