中国老年学杂志
中國老年學雜誌
중국노년학잡지
CHINESE JOURNAL OF GERONTOLOGY
2010年
4期
492-494
,共3页
张慕蕊%王岩%赵东旭%李新娜%李群%李玉林
張慕蕊%王巖%趙東旭%李新娜%李群%李玉林
장모예%왕암%조동욱%리신나%리군%리옥림
逆转录病毒%稳定转染
逆轉錄病毒%穩定轉染
역전록병독%은정전염
Retrovirus vector%Stable transfected cell line
目的 应用 Slingshot(SSH)-1L重组逆转录病毒载体,建立过表达该基因的稳定转染细胞系.方法 用脂质体法将含SSH-1L的重组逆转录病毒载体pLNCX-SSH-1L转染PA317包装细胞,G418筛选,滴度测定.收集病毒上清感染MG63细胞,G418筛选.结果 应用 pLNCX-Slingshot-1L重组逆转录病毒载体,获得滴度为5×10~8 CFU /L的病毒上清,感染MG63细胞,获得了过表达SSH-1L基因的MG63细胞.结论 应用SSH-1L重组逆转录病毒载体,成功建立了过表达该基因的稳定细胞系.
目的 應用 Slingshot(SSH)-1L重組逆轉錄病毒載體,建立過錶達該基因的穩定轉染細胞繫.方法 用脂質體法將含SSH-1L的重組逆轉錄病毒載體pLNCX-SSH-1L轉染PA317包裝細胞,G418篩選,滴度測定.收集病毒上清感染MG63細胞,G418篩選.結果 應用 pLNCX-Slingshot-1L重組逆轉錄病毒載體,穫得滴度為5×10~8 CFU /L的病毒上清,感染MG63細胞,穫得瞭過錶達SSH-1L基因的MG63細胞.結論 應用SSH-1L重組逆轉錄病毒載體,成功建立瞭過錶達該基因的穩定細胞繫.
목적 응용 Slingshot(SSH)-1L중조역전록병독재체,건립과표체해기인적은정전염세포계.방법 용지질체법장함SSH-1L적중조역전록병독재체pLNCX-SSH-1L전염PA317포장세포,G418사선,적도측정.수집병독상청감염MG63세포,G418사선.결과 응용 pLNCX-Slingshot-1L중조역전록병독재체,획득적도위5×10~8 CFU /L적병독상청,감염MG63세포,획득료과표체SSH-1L기인적MG63세포.결론 응용SSH-1L중조역전록병독재체,성공건립료과표체해기인적은정세포계.
Objective To establish stable transfected cell line by retroviral vector encoding Slingshot-1L.Methods The plasmid pLNCX-Slingshot-1L was transfected into packaging cell PA317 by lipofectamine. The transfectants were selected by G418 and viral titers were analyzed. Virals supernatant were collected to infect MG63 sells, selected by G418. Results Virals supernatant with high viral titers were obtained by retroviral vector encoding Slingshot-1L. Virals supernatant were collected to infect MG63 sells, and the stable transfected MG63 cell line was established. Conclusions The stable transfected cell line over-expression Slingshot-1L mediated by retroviral is established successfully.
