CAJ | 학술논문

背景:毛囊干细胞在受到外界环境刺激作用下,例如创伤,化学伤等,其珲化性质及细胞状态会有所变化.但是从目前文献来看,涉及其在皮肤烫伤中变化的研究的比较少.目的:初步观察大鼠烫伤模型中,毛囊隆突区β神经生长因了的表达变化以及对烫伤组织愈合的影响.方法:随机选取30只乳鼠,采用90℃水温持续3 s时间的方式制作大鼠触须部表皮组织浅Ⅱ度烫伤模型,造模后第12,24,36,48,60小时对毛囊隆突区进行分离,并进行匀浆,以同窝新生6只乳鼠正常唇部组织作为对照.采用Brandford法进行总蛋白检测及ELISA定量检测β神经生长因子.免疫组织化学进行神经生长因子的定性半定量检测.结果与结论:在烫伤后各时间点检测的β神经生长因子与总蛋白比值中,β神经生长因子的表达在烫伤后36 h开始后增高,60 h达到峰值.组织切片中同样发现这一规律.通过图像软件分析表明,提取β神经生长因子蛋白检测与组织水平β神经生长因子表达一致.提示烫伤后12～60 h时间段内,烫伤后60 h隆突区表达的β神经生长因子达到峰值,对烫伤愈合的修复作用也达到峰值.隆突区是毛囊干细胞的巢,此部位β神经生长因子表达增强可能对毛囊干细胞的分化和组织修复具有一定的积极意义.
배경:모낭간세포재수도외계배경자격작용하,례여창상,화학상등,기혼화성질급세포상태회유소변화.단시종목전문헌래간,섭급기재피부탕상중변화적연구적비교소.목적:초보관찰대서탕상모형중,모낭륭돌구β신경생장인료적표체변화이급대탕상조직유합적영향.방법:수궤선취30지유서,채용90℃수온지속3 s시간적방식제작대서촉수부표피조직천Ⅱ도탕상모형,조모후제12,24,36,48,60소시대모낭륭돌구진행분리,병진행균장,이동와신생6지유서정상진부조직작위대조.채용Brandford법진행총단백검측급ELISA정량검측β신경생장인자.면역조직화학진행신경생장인자적정성반정량검측.결과여결론:재탕상후각시간점검측적β신경생장인자여총단백비치중,β신경생장인자적표체재탕상후36 h개시후증고,60 h체도봉치.조직절편중동양발현저일규률.통과도상연건분석표명,제취β신경생장인자단백검측여조직수평β신경생장인자표체일치.제시탕상후12～60 h시간단내,탕상후60 h륭돌구표체적β신경생장인자체도봉치,대탕상유합적수복작용야체도봉치.륭돌구시모낭간세포적소,차부위β신경생장인자표체증강가능대모낭간세포적분화화조직수복구유일정적적겁의의.
BACKGROUND:The surroundings stimulation,such as wound and chemical injury,will result in changes of hair follicle stem cells(HFSC).Up to date,few articles indicate the effecl of burn on HFSCs.OBJECTIVE:To investigate the changes of β-nerve growth factor(β-NGF)level in vibrissa follicle bulge region and its effect on the healing of neonatal rats.METHODS:A total of 30 neonatal rats were selected to prepare burn models by giving 90℃ boiled tap water for 3 seconds,and vibrissa follicle bulge was separated at 12,24,36,48 and 60 hours pOsloperatiVeIy.The remained 6 neonatal rats were served as controls.Total protein concentration was measured by Brandford method The expression of β-NGF was detected by ELISA.Meanwhile the tissues in each time points were collected for frozen section and immunohistochemistry.RESULTS AND CONCLUSION:Among the tested total protein.the expression of the β-NGF increased after 36 hours.reached a peak at 60 hours after burn.The identical changes were found in tissue slices.The image analysis demonstrated that the β-NGF protein detection was coincident with β-NGF expression in tissues During 12 hours to 60 hours after burn,the expression of the β-NGF in the bulge area peaked at 60 hours;simultaneously,the repairing effect of healing also reached a peak.Bulge area is a nest of HFSC,the enhanced expression of β-NGF may have some positive significance to the differentiation of the HFSC and tissue repair.