华南农业大学学报
華南農業大學學報
화남농업대학학보
JOURNAL OF SOUTH CHINA AGRICULTURAL UNIVERSITY
2010年
2期
100-103
,共4页
牟希东%王培欣%胡隐昌%汪学杰%宋红梅%李小慧%罗建仁
牟希東%王培訢%鬍隱昌%汪學傑%宋紅梅%李小慧%囉建仁
모희동%왕배흔%호은창%왕학걸%송홍매%리소혜%라건인
骨舌鱼科%美丽硬仆骨舌鱼%细胞色素b基因%系统进化
骨舌魚科%美麗硬僕骨舌魚%細胞色素b基因%繫統進化
골설어과%미려경부골설어%세포색소b기인%계통진화
Osteoglossidae%Scleropages formosus%Cyt b gene%systematical evolution
为阐明骨舌鱼科鱼类的遗传结构和进化关系,测定了美丽硬仆骨舌鱼Scleropages formosus的3个品种金龙(Gold arowana)、红龙(Red arowana)、青龙(Green arowana)细胞色素b(cyt b)基因全序列(1 141 bp),结合来自GenBank中珍珠龙Scleropages leichardti、星点珍珠龙S. jardini、非洲龙Heterotis niloticus、黑龙Osteoglossum ferrerirai、银龙O. bicirrhosum、海象Arapaima gigas的全序列,用邻接法(NJ)和最大简约法(MP)构建分子系统树,初步分析了骨舌鱼科鱼类的系统发育关系.NJ树和 MP树均一致表明,骨舌鱼类为单系类群,分为4支,支持骨舌鱼科下设4个属(坚体鱼属Scleropages、异耳鱼属Heterotis、骨舌鱼属Osteoglossum、巨骨舌鱼属Arapaima)阶元的分类系统,4个属的系统关系与其形态、生态特征表型进化和地理分布较为一致.而金龙、红龙和青龙的mtDNA Cytb的碱基差异小于1%,说明这3个龙鱼品种还在同一种的水平上.
為闡明骨舌魚科魚類的遺傳結構和進化關繫,測定瞭美麗硬僕骨舌魚Scleropages formosus的3箇品種金龍(Gold arowana)、紅龍(Red arowana)、青龍(Green arowana)細胞色素b(cyt b)基因全序列(1 141 bp),結閤來自GenBank中珍珠龍Scleropages leichardti、星點珍珠龍S. jardini、非洲龍Heterotis niloticus、黑龍Osteoglossum ferrerirai、銀龍O. bicirrhosum、海象Arapaima gigas的全序列,用鄰接法(NJ)和最大簡約法(MP)構建分子繫統樹,初步分析瞭骨舌魚科魚類的繫統髮育關繫.NJ樹和 MP樹均一緻錶明,骨舌魚類為單繫類群,分為4支,支持骨舌魚科下設4箇屬(堅體魚屬Scleropages、異耳魚屬Heterotis、骨舌魚屬Osteoglossum、巨骨舌魚屬Arapaima)階元的分類繫統,4箇屬的繫統關繫與其形態、生態特徵錶型進化和地理分佈較為一緻.而金龍、紅龍和青龍的mtDNA Cytb的堿基差異小于1%,說明這3箇龍魚品種還在同一種的水平上.
위천명골설어과어류적유전결구화진화관계,측정료미려경부골설어Scleropages formosus적3개품충금룡(Gold arowana)、홍룡(Red arowana)、청룡(Green arowana)세포색소b(cyt b)기인전서렬(1 141 bp),결합래자GenBank중진주룡Scleropages leichardti、성점진주룡S. jardini、비주룡Heterotis niloticus、흑룡Osteoglossum ferrerirai、은룡O. bicirrhosum、해상Arapaima gigas적전서렬,용린접법(NJ)화최대간약법(MP)구건분자계통수,초보분석료골설어과어류적계통발육관계.NJ수화 MP수균일치표명,골설어류위단계류군,분위4지,지지골설어과하설4개속(견체어속Scleropages、이이어속Heterotis、골설어속Osteoglossum、거골설어속Arapaima)계원적분류계통,4개속적계통관계여기형태、생태특정표형진화화지리분포교위일치.이금룡、홍룡화청룡적mtDNA Cytb적감기차이소우1%,설명저3개룡어품충환재동일충적수평상.
The present study aimed to clarify the genetic structure and evolutionary relationships within Osteoglossidae,using complete mitochondrial cytochrome b(Cyt b) gene sequences (1 141 bp) of three breeds of Scleropages formosus:Gold arowana,Red arowana and Green arowana,together with relevant sequences of Scleropages leichardti,S. jardini,Heterotis niloticus,Osteoglossum ferrerirai,O. bicirrhosum,Arapaima gigas obtained from GenBank.The phylogenetic trees constructed by two methods (NJ and MP) supported the same topology,and molecular phylogenetic relationships of fishes of the Osteoglossidae were constructed.Both of NJ tree and MP tree showed that fishes of the Osteoglossidae were a monophyletic group and classified Osteoglossidae into four genera,namely Scleropages,Heterotis,Osteoglossum,Arapaima,which were perfectly consistent with their morphological,ecological characteristics,evolution and geographic distribution pattern.Sequence variation of less than 1% in mtDNA Cyt b gene among three breeds of S. formosus suggested that they might represent the same species.