中国动物保健
中國動物保健
중국동물보건
CHINA ANIMAL HEALTH
2011年
9期
12-13,15
,共3页
马燕%王纯洁%敖日格乐%姜艳%冉金宝%裴大为
馬燕%王純潔%敖日格樂%薑豔%冉金寶%裴大為
마연%왕순길%오일격악%강염%염금보%배대위
蒙药三子汤%热应激%小鼠%CD3+T细胞%CD19+B细胞
矇藥三子湯%熱應激%小鼠%CD3+T細胞%CD19+B細胞
몽약삼자탕%열응격%소서%CD3+T세포%CD19+B세포
Mongolian medicine%heat stress%mice%CD3 +T cell%CD 19 +B cell
为了研究蒙药三子汤对热应激小鼠的CD3+T/CD19+B细胞的作用,160只小鼠随机分4组:灌水组、热应激组、低剂量热应激组和高剂量热应激组,热应激后眼前房采血,流式细胞仪检测CD3+T/CD19+B细胞变化。结果:热应激0h的4组小鼠CD3+T/CD19+B细胞差异不显著;热应激组CD3+细胞2h、7d均显著低于灌药、灌水组(p〈0.05);灌药组1h、2h、7d小鼠CD19+细胞量显著高于热应激、灌水组。表明三子汤可以维持热应激小鼠CD3+T细胞量无显著变化,并可显著提高CD19+B细胞量。
為瞭研究矇藥三子湯對熱應激小鼠的CD3+T/CD19+B細胞的作用,160隻小鼠隨機分4組:灌水組、熱應激組、低劑量熱應激組和高劑量熱應激組,熱應激後眼前房採血,流式細胞儀檢測CD3+T/CD19+B細胞變化。結果:熱應激0h的4組小鼠CD3+T/CD19+B細胞差異不顯著;熱應激組CD3+細胞2h、7d均顯著低于灌藥、灌水組(p〈0.05);灌藥組1h、2h、7d小鼠CD19+細胞量顯著高于熱應激、灌水組。錶明三子湯可以維持熱應激小鼠CD3+T細胞量無顯著變化,併可顯著提高CD19+B細胞量。
위료연구몽약삼자탕대열응격소서적CD3+T/CD19+B세포적작용,160지소서수궤분4조:관수조、열응격조、저제량열응격조화고제량열응격조,열응격후안전방채혈,류식세포의검측CD3+T/CD19+B세포변화。결과:열응격0h적4조소서CD3+T/CD19+B세포차이불현저;열응격조CD3+세포2h、7d균현저저우관약、관수조(p〈0.05);관약조1h、2h、7d소서CD19+세포량현저고우열응격、관수조。표명삼자탕가이유지열응격소서CD3+T세포량무현저변화,병가현저제고CD19+B세포량。
To study the influence of Mongolian medicine Sanzitang to CD3 +T/CD19+B cell of heat stress mice, 160 mice were divided into 4 groups randomly. Water drinking group, heat stress group, low dosage-heat stress group and high dosage-heat stress group. Blood was picked off from their eye pre-room after heat stress. To examine the change of C D3 +T and C D 19+B cell with Flow Cytometery. Results shows there are no significant difference in CD3 +T/CD19+B cell of 4 groups of Oh heat stress .The CD3 +T cell of heat stress groups after 2h/Td heat stress were lower than Mongolian medicine and water drinking groups significantly(p 〈 0.05 ). CD 19+B cell of 2 Mongolian medicine groups were higher than heat stress and water drinking groups significantly (p 〈 0.05) after heat stress lh/2h/7d. These demonstrated that Sanzitang can maitain CD3 +T cell of heat stress mice no decrease, and increase CD19 +B cell of heat stress mice significantly.