CAJ | 학술논문

目的探讨苦参素对活化肝星状细胞(HSC)整合素αv的表达和矢巢凋亡的影响.方法把培养激活的大鼠肝星状细胞株(HSC-T6)分为实验组和对照组,实验组以不同浓度的苦参素干预,蛋白质印迹(Western Blotting)法检测整合素αv的表达,末端脱氧核苷酸转移酶介导的脱氧三磷酸尿苷缺口末端标记法(TUNUL)检测凋亡指数.结果药物干预后整合素αv的表达随干预浓度的增加而逐渐降低(P＜0.05),凋亡指数则逐渐增高(P＜0.05),且呈浓度依赖性(r分别为-0.76、0.81);整合素αv与凋亡之间呈负相关,r为-0.79.结论苦参素能通过抑制活化HSC的整合素αv的表达促进其凋亡,在肝纤维化治疗中具有重要意义.
목적 탐토고삼소대활화간성상세포(HSC)정합소αv적표체화시소조망적영향.방법 파배양격활적대서간성상세포주(HSC-T6)분위실험조화대조조,실험조이불동농도적고삼소간예,단백질인적(Western Blotting)법검측정합소αv적표체,말단탈양핵감산전이매개도적탈양삼린산뇨감결구말단표기법(TUNUL)검측조망지수.결과 약물간예후정합소αv적표체수간예농도적증가이축점강저(P＜0.05),조망지수칙축점증고(P＜0.05),차정농도의뢰성(r분별위-0.76、0.81);정합소αv여조망지간정부상관,r위-0.79.결론 고삼소능통과억제활화HSC적정합소αv적표체촉진기조망,재간섬유화치료중구유중요의의.
Objective To explore the effect of Kwoninone on integrin αv Expression and anoikis in activated hepatic stellate cells.Methods HSC T6 was incubated and divided into control group and study group.Western Blotting and TUNUL were employed to estimate the Expression of integrin αv and apoptosis of HSC T6.Results When kwoninone was used,the expression of integrin αv significantly decreased in study group,compared with control group(P＜0.05),and the apoptosis index was higher after treatment with kwoninone.There was a significantly negative correlation between integrin αv and anoikis(r=-0.79).Conclusion Kwoninone can induce the apoptosis of HSCT6 by inhibiting expression of integrin αv.