中华劳动卫生职业病杂志
中華勞動衛生職業病雜誌
중화노동위생직업병잡지
CHINESE JOURNAL OF INDUSTRIAL HYGIENE AND OCCUPATIONAL DISEASES
2011年
3期
227-229
,共3页
焦路阳%宋志善%郭庆合%鲁广建
焦路暘%宋誌善%郭慶閤%魯廣建
초로양%송지선%곽경합%로엄건
百草枯%肿瘤坏死因子%白细胞介素类
百草枯%腫瘤壞死因子%白細胞介素類
백초고%종류배사인자%백세포개소류
Paraquat%Tumor necrosis factor%Interlerleukins
目的 观察急性百草枯(PQ)中毒大鼠血清中炎性因子的变化,探讨炎性因子在急性PQ中毒肾损伤中的作用.方法 100只SD雄性大鼠随即分成正常对照组(20只,生理盐水腹腔注射)和PQ染毒组(80只,腹腔注射PQ 25mg/kg).PQ组于染毒后6h及1、3、7 d处死,正常对照组于处理后1d处死,检测血清中肿瘤坏死因子-α(TNF-α)、白细胞介素-2(IL-2)、IL-6含量,并观察肾组织的病理改变.结果 PQ染毒组大鼠肾组织早期病理表现为充血水肿,少量炎性细胞浸润,染毒组在不同时间点病理损伤的严重程度无明显差别,死亡大鼠肾组织细胞核固缩、细胞结构溶解等病理改变.与正常对照组相比,PQ染毒组大鼠6 h和1、3、7 d时IL-2水平分别为(2.16±0.65)、(2.95±1.02)、(3.05±1.12)、(2.21±0.62)μg/L,IL-6分别为(62.5±8.6)、(85.6±13.5)、(90.3±15.6)、(65.3±9.1)ng/ml,TNF-α分别为(1.95±0.53)、(2.86±0.92)、(3.15±1.02)、(2.06±0.71)μg/L,与对照组[IL-2:(1.71±0.45)μg/L,IL-6(38.2±7.5)ng/ml,TNF-α(1.22±0.32)μg/L比较,均明显升高,差异均有统计学意义(P＜0.01).结论 急性PQ中毒大鼠血清中TNF-α、IL-2、IL-6水平明显升高,均参与PQ急性中毒肾损伤的致病过程.
目的 觀察急性百草枯(PQ)中毒大鼠血清中炎性因子的變化,探討炎性因子在急性PQ中毒腎損傷中的作用.方法 100隻SD雄性大鼠隨即分成正常對照組(20隻,生理鹽水腹腔註射)和PQ染毒組(80隻,腹腔註射PQ 25mg/kg).PQ組于染毒後6h及1、3、7 d處死,正常對照組于處理後1d處死,檢測血清中腫瘤壞死因子-α(TNF-α)、白細胞介素-2(IL-2)、IL-6含量,併觀察腎組織的病理改變.結果 PQ染毒組大鼠腎組織早期病理錶現為充血水腫,少量炎性細胞浸潤,染毒組在不同時間點病理損傷的嚴重程度無明顯差彆,死亡大鼠腎組織細胞覈固縮、細胞結構溶解等病理改變.與正常對照組相比,PQ染毒組大鼠6 h和1、3、7 d時IL-2水平分彆為(2.16±0.65)、(2.95±1.02)、(3.05±1.12)、(2.21±0.62)μg/L,IL-6分彆為(62.5±8.6)、(85.6±13.5)、(90.3±15.6)、(65.3±9.1)ng/ml,TNF-α分彆為(1.95±0.53)、(2.86±0.92)、(3.15±1.02)、(2.06±0.71)μg/L,與對照組[IL-2:(1.71±0.45)μg/L,IL-6(38.2±7.5)ng/ml,TNF-α(1.22±0.32)μg/L比較,均明顯升高,差異均有統計學意義(P＜0.01).結論 急性PQ中毒大鼠血清中TNF-α、IL-2、IL-6水平明顯升高,均參與PQ急性中毒腎損傷的緻病過程.
목적 관찰급성백초고(PQ)중독대서혈청중염성인자적변화,탐토염성인자재급성PQ중독신손상중적작용.방법 100지SD웅성대서수즉분성정상대조조(20지,생리염수복강주사)화PQ염독조(80지,복강주사PQ 25mg/kg).PQ조우염독후6h급1、3、7 d처사,정상대조조우처리후1d처사,검측혈청중종류배사인자-α(TNF-α)、백세포개소-2(IL-2)、IL-6함량,병관찰신조직적병리개변.결과 PQ염독조대서신조직조기병리표현위충혈수종,소량염성세포침윤,염독조재불동시간점병리손상적엄중정도무명현차별,사망대서신조직세포핵고축、세포결구용해등병리개변.여정상대조조상비,PQ염독조대서6 h화1、3、7 d시IL-2수평분별위(2.16±0.65)、(2.95±1.02)、(3.05±1.12)、(2.21±0.62)μg/L,IL-6분별위(62.5±8.6)、(85.6±13.5)、(90.3±15.6)、(65.3±9.1)ng/ml,TNF-α분별위(1.95±0.53)、(2.86±0.92)、(3.15±1.02)、(2.06±0.71)μg/L,여대조조[IL-2:(1.71±0.45)μg/L,IL-6(38.2±7.5)ng/ml,TNF-α(1.22±0.32)μg/L비교,균명현승고,차이균유통계학의의(P＜0.01).결론 급성PQ중독대서혈청중TNF-α、IL-2、IL-6수평명현승고,균삼여PQ급성중독신손상적치병과정.
Objective To investigate the change of inflammatory factor in lung tissue of acute paraquat (PQ) poisoned rats. Methods hundred SD rats were randomizedly divided into two groups:the normal control group (NC group, n= 10 )and the PQ group (n=80).The 1 ml saline was administered once in normal control group.The PQ group was administered with 25 mg/kg 1% PQ by intraperitoneal injection to establish the model of PQ induced renal injury. At six hours,at the first,the third and the seventh day the PQ group were sacrificed,while at the first day the normal control group was sacrificed. The level of normal tumor necrosis factor-a (TNF-α), interleukin -2 (IL-2), IL-6 in serum of rats were detected. Meanwhile, pathological changes of the renal were examined under optical microscope. Results Histopathological findings of an earlier, a large number of patients edema clearly inflammatory cell infiltration. Compared with the control group,PQ exposure of serum TNF-α, IL-2, IL-6, the level at each time point were elevated. PQ treated group 6 h and 1,3, 7 d when the IL-2 levels were (2.16±0.65), (2.95±1.02), (3.05±1.12), (2.21±0.62)μg/L, IL-6 were (62.5±8.6), (85.6±13.5), (90.3±15.6), (65.3±9.1)ng/ml, TNF-α were (1.95±0.53), (2.86±0.92), (3.15±1.02),(2.06 ±0.71 )μg/L, compared with the control group, are significantly higher, the differences were statistically significant (P＜0.01). Conclusion acute PQ poisoning serum TNF-α, IL-2, IL-6 levels were significantly increased both early and late inflammatory factors involved in PQ poisoning the pathogenesis of renal injury.
