CAJ | 학술논문

目的探讨内皮素1对大肠癌HCT-116细胞增殖的影响.方法应用RT-PCR及免疫组织化学法检测大肠癌HCT-116细胞内皮素1,内皮素受体A、B表达情况;应用MTY法检测不同浓度和作用时间的内皮素1对体外培养大肠癌HCT-116细胞增殖的影响,多组比较采用方差分析.结果 HCT-116细胞存在内皮素1及内皮素受体A、B的基因和蛋白表达;内皮素1能明显促进大肠癌HCT-116细胞的增殖,在干预48 h且浓度为1×10-7mol/L时达到最强.结论内皮素1通过细胞膜上受体的介导,促进HCT-116细胞的增殖,呈药物浓度依赖性.
목적 탐토내피소1대대장암HCT-116세포증식적영향.방법 응용RT-PCR급면역조직화학법검측대장암HCT-116세포내피소1,내피소수체A、B표체정황;응용MTY법검측불동농도화작용시간적내피소1대체외배양대장암HCT-116세포증식적영향,다조비교채용방차분석.결과 HCT-116세포존재내피소1급내피소수체A、B적기인화단백표체;내피소1능명현촉진대장암HCT-116세포적증식,재간예48 h차농도위1×10-7mol/L시체도최강.결론 내피소1통과세포막상수체적개도,촉진HCT-116세포적증식,정약물농도의뢰성.
Objective To investigate the effects of endothelin-1 on the proliferation of HCT-116 cells. Methods The expression of endothelin-1 and endothelin receptors A and B in the HCT-116 cells was detected by RT-PCR and immunohistochemistry method.The effects of endothelin-1 at different concentrations and action time points on HCT-116 cells were measured by MTT assay and were analyzed by variance analysis.Results The expression of endothelin-1 and endothelin receptors A and B at the gene and protein levels in HCT-116 cells was detected.Endothelin-1 had significant effects on the proliferation of HCT-116 cells.The proliferation peak of HCT-116 cells was reached when the concentration of endothelin-1 was 1×10-7mol/L and the action time was 48 hours.Conclusions Endothelin-1 promotes proliferation of HCT-116 cells in a dose-and time-dependent manner,and the process is mediated by endothelin receptors.