CAJ | 학술논문

目的了解WU多瘤病毒(WU polyomaviros,WUPyV)在小儿呼吸道感染中的检出情况及感染患儿的临床特点.方法应用PCR技术,对2008年7月至2009年6月广东省汕头大学医学院第二附属医院儿科急性呼吸道感染住院患儿的呼吸道鼻咽抽取液及健康体检儿童呼吸道鼻咽抽取液进行WUPyV核酸检测,阳性结果再进一步基因测序,并分析阳性患儿的临床资料.结果 771例患儿中WUPyV阳性15例,阳性率1.95%,其中单纯WUPyV感染9例(60%),合并其他病毒感染的有6例(40%).82例健康体检儿童WUPyV的PCR检查均阴性.所有WUPyV阳性患儿年龄均≤4岁,最小2个月,最大48个月,平均年龄18.8个月;男: 女=1.5:1,临床诊断主要是:支气管肺炎、细支气管炎、上呼吸道感染、支气管炎,其中1例重症患儿合并病毒性脑炎.结论 WUPyV可能是呼吸道感染病原体,其主要感染对象是婴幼儿,主要症状是咳嗽和喘息,个别有重症表现.
목적 료해WU다류병독(WU polyomaviros,WUPyV)재소인호흡도감염중적검출정황급감염환인적림상특점.방법 응용PCR기술,대2008년7월지2009년6월광동성산두대학의학원제이부속의원인과급성호흡도감염주원환인적호흡도비인추취액급건강체검인동호흡도비인추취액진행WUPyV핵산검측,양성결과 재진일보기인측서,병분석양성환인적림상자료.결과 771례환인중WUPyV양성15례,양성솔1.95%,기중단순WUPyV감염9례(60%),합병기타병독감염적유6례(40%).82례건강체검인동WUPyV적PCR검사균음성.소유WUPyV양성환인년령균≤4세,최소2개월,최대48개월,평균년령18.8개월;남: 녀=1.5:1,림상진단주요시:지기관폐염、세지기관염、상호흡도감염、지기관염,기중1례중증환인합병병독성뇌염.결론 WUPyV가능시호흡도감염병원체,기주요감염대상시영유인,주요증상시해수화천식,개별유중증표현.
Objective WU polyomavirus (WUPyV), a new member of the genus Polyomavirus in the family Polyomaviridae, has been found to be associated with respiratory tract infections recently. But the role of the WUPyV as agents of human disease remains uncertain. We sought to describe the detection and clinical characterization of WUPyV in acute respiratory tract infection in children. Method From July 2008 through June 2009, nasopharyngeal aspirates were collected from 771 children who were hospitalized with acute respiratory tract infection in Second Affiliated Hospital of Shantou University Medical College, and from 82 asymptomatic children who visited the health checkup clinic. WUPyV was detected by using PCR technology and was identified by using DNA sequencing. All WUPyV-positive specimens were screened for 9 common viruses [influenza A and B, respiratory syncytial virus (RSV), parainfluenza virus (PIV) 1 and 3, human metapneumovirus, human bocavirus, adenovirus and rhinovirus] by using PCR or RT-PCR. The clinical data of WUPyV infection were collected and analyzed. Result In this study, fifteen of the 771 tested specimens with acute respiratory tract infection were positive for WUPyV, the positive rate was 1.95% and all of the asymptomatic children who visited the health checkup clinic were negative. Of the 15 cases who were positive for the virus, the age range was 2 to 48 (mean 18.8) months, 9 (60%) were male and 6 (40%) were female. WUPyV was the sole virus detected in 9 specimens (60%) from patients with acute respiratory tract infection. WUPyV was associated with the co-infection with another respiratory virus in 6 of 15 (40%) cases, most frequently with RSV (n =4), followed by adenovirus (n = 1) and rhinovirus (n = 1). The most common clinical findings in the patients with WUPyV were cough, fever and wheezing. The most frequent diagnoses were pneumonia (n = 8), bronchiolitis (n = 4), upper respiratory tract infections (n = 2) and bronchitis (n = 1). A severe case was complicated with viral encephalitis. Conclusion WUPyV may be a respiratory pathogen because it was the sole virus detected in 9 specimens from patients with respiratory illness and all of the asymptomatic controls were negative. The most common clinical findings are cough and wheezing. Young children may be susceptible to infection with this virus and occasionally the infection with this virus may cause severe disease. More comprehensive and in-depth studies are required to prove the pathogenicity of these viruses.