CAJ | 학술논문

目的探讨DNA损伤修复基因—X线修复交叉互补基因1(XRCCI)多态性与苯致外周血淋巴细胞染色体损伤的关系.方法采用胞质分裂阻滞微核试验方法评价459名苯接触工人和88名非接苯对照组工人染色体损伤水平,应用聚合酶链反应-限制性片段多态性技术检测XRCCI第6外显子194密码子,第9外显子280密码子和第10外显子399密码子多态.结果接苯组微核发生率(2.12‰±1.88‰)明显高于对照组(1.19‰±1.68‰),差异有统计学意义(P＜0.01);＞35岁年龄组的接苯工人微核发生率(3.00‰±2.76‰)明显高于≤35岁年龄组(2.02‰±1.71‰),差异有统计学意义(P＜0.05).未发现XRCCI基因多态性与微核率有关.与AAMAAA野生型比较,携带AAA/BAA、AAB/AAB、ABA/ABA及ABB/ABB双体型的接苯工人微核率明显升高,差异有统计学意义(P＜0.05).结论苯接触能致作业工人染色体损伤.年龄及XRCC1基因双体型与苯致染色体损伤有关.
목적 탐토DNA손상수복기인—X선수복교차호보기인1(XRCCI)다태성여분치외주혈림파세포염색체손상적관계.방법 채용포질분렬조체미핵시험방법평개459명분접촉공인화88명비접분대조조공인염색체손상수평,응용취합매련반응-한제성편단다태성기술검측XRCCI제6외현자194밀마자,제9외현자280밀마자화제10외현자399밀마자다태.결과 접분조미핵발생솔(2.12‰±1.88‰)명현고우대조조(1.19‰±1.68‰),차이유통계학의의(P＜0.01);＞35세년령조적접분공인미핵발생솔(3.00‰±2.76‰)명현고우≤35세년령조(2.02‰±1.71‰),차이유통계학의의(P＜0.05).미발현XRCCI기인다태성여미핵솔유관.여AAMAAA야생형비교,휴대AAA/BAA、AAB/AAB、ABA/ABA급ABB/ABB쌍체형적접분공인미핵솔명현승고,차이유통계학의의(P＜0.05).결론 분접촉능치작업공인염색체손상.년령급XRCC1기인쌍체형여분치염색체손상유관.
Objective To explore the relationship between the polymorphisms of DNA repair gene (XRCCI 194,280 and 399) and the chromosomal damage induced by benzene.Methods The chromosomal damage of the peripheral lymphocytes in 459 workers occupationally exposed to benzene and 88 non-exposed controls were detected with cytokinesis-block micronucleus (CBMN) assay.PCR-RFLP technique was used to measure polymorphisms in XR CCI 194,280 and 399.Results It was found that the MN frequency (2.12‰±1.88‰)of the exposed group was significantly higher than that (1.19‰±1.68‰) of the control group (P＜0.05),in the exposed group,the M N frequency (3.00‰±2.76‰) of older workers ( ＞35 years ) was significantly higher than that (2.02‰±1.71‰) of younger workers (≤35 years) (P＜0.05).The effect of genetic polymorphisms of XRCCI on CBMN was not found.The haplotypes AAA/BAA,AAB/AAB,ABA/ABA,ABB/ABB could associated with the increased frequencies of total micronucleus (P＜0.05).Conclusion Benzene exposure could result in chromosome damage.Age of workers and diplotypes of XRCCI could associated with chromosomal damage induced by benzene.