笃斯越橘对兔眼光损伤前后视网膜电流图及组织结构的影响
독사월귤대토안광손상전후시망막전류도급조직결구적영향
The effect of Vaccinium uliginosum to the electroretinogram and retina of rabbits before and after light-induced damage
  • 万方数据
    中华眼科杂志 중화안과잡지
    Chinese Journal of Ophthalmology
    2010年 5期 446-451 ,共6页

    尹澜%皮裕琍%张卯年

    윤란%피유리%장묘년

    越橘属%植物制剂%眼烧伤%视网膜电描记术%视网膜 越橘屬%植物製劑%眼燒傷%視網膜電描記術%視網膜
    월귤속%식물제제%안소상%시망막전묘기술%시망막
    Vaccinium%Plant preparations%Eye burns%Electroretinography%Retina
    目的 探讨笃斯越橘对兔眼光损伤前后视网膜电流图(ERG)及组织结构的影响.方法 对照实验研究.采用随机数字表法将35只青紫蓝兔分为5组,每组7只兔.各组自由进食、摄水,对A、D组兔加用笃斯越橘匀浆.4周后,在光镜下观察D、E组兔视网膜组织学结构变化并测量光感受器细胞外核层(ONL)厚度及凋亡指数(AI);按照国际临床视觉电生理学会标准化方案确立的最大混合反应ERG及振荡电位(Ops)对A、B、C组兔视网膜进行检测.随后进行光损伤照射.照射后A组停用笃斯越橘,C组加用笃斯越橘,B组保持不变.在光照后1 d、1和2周进行ERG检测.检测完毕,取A、B、C组兔视网膜进行组织学分析.对各组兔视网膜ERG潜伏期、振幅、ONL厚度及AI值比较采用双因素及单因素的方差分析,进一步两两比较采用LSD检验法.结果 (1)最大混合反应ERG检测结果:A组饲养4周后,潜伏期:a波(14.079±0.841)ms,b波(35.629±6.865)ms;振幅:a波(83.936±10.807)μV,b波(280.931±27.807)μV.A组光照2周后,潜伏期:a波(15.571±1.087)ms,b波(38.915±7.683)ms;振幅:a波(66.478±9.709)μV,b波(245.887±11.797)μV.B组饲养4周后,潜伏期:a波(15.635±1.661)ms,b波(42.985±3.164)ms;振幅:a波(69.331±12.355)μV,b波(197.331±16.157)μV.B组光照2周后,潜伏期:a波(18.783±1.966)ms,b波(52.322±4.784)ms;振幅:a波(57.562±8.217)μV,b波(159.569±17.859)μV.C组饲养4周后,潜伏期:a波(15.115±0.940)ms,b波(43.242±4.662)ms;振幅:a波(72.812±4.403)μV,b波(207.815±14.373)μV.C组光照2周后,潜伏期:a波(15.957±2.154)ms,b波(44.081±9.506)ms;振幅:a波(66.804±8.755)μV,b波(186.271±29.349)μV.A、B、C组间最大混合反应ERG在饲养4周后及光照2周后差异有统计学意义(潜伏期a波:饲养4周后F=6.057,P＜0.05;光照2周后F=13.296,P＜0.05.潜伏期b波:饲养4周后F=9.949,P＜0.05;光照2周后F=11.145,P＜0.05.振幅a波:饲养4周后F=8.468,P＜0.05;光照2周后F=4.844,P＜0.05.振幅b波:饲养4周后F=70.194,P＜0.05;光照2周后F=62.161,P＜0.05);3组间ΣOPs值振幅差异有统计学意义(饲养4周后F=17.482,P＜0.05;光照2周后F=11.748,P＜0.05).进一步两两比较,显示光照前最大混合反应ERG振幅B组的a、b波及C组的a、b波均低于笃斯越橘饲养4周后的A组(B组的a波、b波振幅与A组比较:P=0.003,0.000;C组的a波、b波振幅与A组比较:P=0.001,0.000);光照1 d、1和2周后,A组的振幅b波较同期B组均明显升高(均P=0.000);光照后随用药时间延长,C组ERG检测值逐渐改善;光照2周后,C组较B组的b波潜伏期缩短,振幅提高(潜伏期P=0.008,振幅P=0.007).(2)视网膜组织学观察结果:光照前在光镜下观察兔视网膜组织,D、E组兔视网膜结构形态正常,光感受器细胞内外节排列整齐规则.B组视网膜结构排列紊乱,有光感受器细胞外节碎解.A、C组视网膜结构改变介于D、E和B组之间.各组间ONL厚度差异有统计学意义(F=330.506,P＜0.05).(3)各组间AI值差异有统计学意义(F=230.126,P＜0.05);B组AI值为(10.960±1.534)%,较各组明显增高(均P=0.000);D组AI值为(1.817±0.203)%,小于E组(P=0.000).结论 笃斯越橘对于暴露于正常昼夜日光交替下的兔视网膜有减少细胞凋亡、减轻光化学损伤的作用;预防性地使用笃斯越橘能明显降低光损伤的程度;光损伤后应用笃斯越橘有助于视网膜组织的修复.
    목적 탐토독사월귤대토안광손상전후시망막전류도(ERG)급조직결구적영향.방법 대조실험연구.채용수궤수자표법장35지청자람토분위5조,매조7지토.각조자유진식、섭수,대A、D조토가용독사월귤균장.4주후,재광경하관찰D、E조토시망막조직학결구변화병측량광감수기세포외핵층(ONL)후도급조망지수(AI);안조국제림상시각전생이학회표준화방안학립적최대혼합반응ERG급진탕전위(Ops)대A、B、C조토시망막진행검측.수후진행광손상조사.조사후A조정용독사월귤,C조가용독사월귤,B조보지불변.재광조후1 d、1화2주진행ERG검측.검측완필,취A、B、C조토시망막진행조직학분석.대각조토시망막ERG잠복기、진폭、ONL후도급AI치비교채용쌍인소급단인소적방차분석,진일보량량비교채용LSD검험법.결과 (1)최대혼합반응ERG검측결과:A조사양4주후,잠복기:a파(14.079±0.841)ms,b파(35.629±6.865)ms;진폭:a파(83.936±10.807)μV,b파(280.931±27.807)μV.A조광조2주후,잠복기:a파(15.571±1.087)ms,b파(38.915±7.683)ms;진폭:a파(66.478±9.709)μV,b파(245.887±11.797)μV.B조사양4주후,잠복기:a파(15.635±1.661)ms,b파(42.985±3.164)ms;진폭:a파(69.331±12.355)μV,b파(197.331±16.157)μV.B조광조2주후,잠복기:a파(18.783±1.966)ms,b파(52.322±4.784)ms;진폭:a파(57.562±8.217)μV,b파(159.569±17.859)μV.C조사양4주후,잠복기:a파(15.115±0.940)ms,b파(43.242±4.662)ms;진폭:a파(72.812±4.403)μV,b파(207.815±14.373)μV.C조광조2주후,잠복기:a파(15.957±2.154)ms,b파(44.081±9.506)ms;진폭:a파(66.804±8.755)μV,b파(186.271±29.349)μV.A、B、C조간최대혼합반응ERG재사양4주후급광조2주후차이유통계학의의(잠복기a파:사양4주후F=6.057,P＜0.05;광조2주후F=13.296,P＜0.05.잠복기b파:사양4주후F=9.949,P＜0.05;광조2주후F=11.145,P＜0.05.진폭a파:사양4주후F=8.468,P＜0.05;광조2주후F=4.844,P＜0.05.진폭b파:사양4주후F=70.194,P＜0.05;광조2주후F=62.161,P＜0.05);3조간ΣOPs치진폭차이유통계학의의(사양4주후F=17.482,P＜0.05;광조2주후F=11.748,P＜0.05).진일보량량비교,현시광조전최대혼합반응ERG진폭B조적a、b파급C조적a、b파균저우독사월귤사양4주후적A조(B조적a파、b파진폭여A조비교:P=0.003,0.000;C조적a파、b파진폭여A조비교:P=0.001,0.000);광조1 d、1화2주후,A조적진폭b파교동기B조균명현승고(균P=0.000);광조후수용약시간연장,C조ERG검측치축점개선;광조2주후,C조교B조적b파잠복기축단,진폭제고(잠복기P=0.008,진폭P=0.007).(2)시망막조직학관찰결과:광조전재광경하관찰토시망막조직,D、E조토시망막결구형태정상,광감수기세포내외절배렬정제규칙.B조시망막결구배렬문란,유광감수기세포외절쇄해.A、C조시망막결구개변개우D、E화B조지간.각조간ONL후도차이유통계학의의(F=330.506,P＜0.05).(3)각조간AI치차이유통계학의의(F=230.126,P＜0.05);B조AI치위(10.960±1.534)%,교각조명현증고(균P=0.000);D조AI치위(1.817±0.203)%,소우E조(P=0.000).결론 독사월귤대우폭로우정상주야일광교체하적토시망막유감소세포조망、감경광화학손상적작용;예방성지사용독사월귤능명현강저광손상적정도;광손상후응용독사월귤유조우시망막조직적수복.
    Objective Study the effect of Vaccinium uliginosum (VU) on the electroretinogram (ERG) and histology of rabbits' retina before and after light-induced damage. Methods It was an experimental study in contrast. Thirty-five Chinchilla Rabbits were divided into five groups according to the randomization tables. All rabbits ate and drank freely except those in group A and D who were fed with VU homogenate. Four weeks later we observed the tissue & structure of the rabbits in group D and E under the light microscope and measured the thickness of their out nuclear layers (ONL) and apoptosis index (AI).At the same time, we recorded maximal combined reaction ERG and oscillatory potentials (oscillatory potentials,Ops) of the left rabbits according to the standards set by the International Society for Clinical Electrophysiology of Vision(ISCEV). Then group A, B and C were exposed to strong light. Also we stopped feeding group A with VU and start to feed group C with it. We recorded ERG of them all after 1 day, 1 week and 2 weeks respectively. Then we observed the tissues &structures of them. SPSS 12. 0 software package was used for one-way or double ways ANOVA and LSD test. Results ( 1 ) Maximal combined reaction ERG: after four weeks feeding implicit time of group A:a wave (14. 079 ± 0. 841 )ms, b wave (35.629 ±6. 865) ms; amplitude:a wave (83. 936 ± 10. 807) μV, b wave (280. 931 ±27. 807) μV. Two weeks after injury implicit time of group A: a wave ( 15. 571 ± 1. 087)ms, b wave (38.915 ±7. 683 ) ms, amplitude:a wave (66. 478 ± 9. 709 ) μV, b wave (245. 887 ± 11. 797 ) μV. After four weeks feeding implicit time of group B:a wave ( 15.635 ± 1. 661 ) ms, b wave (42. 985 ± 3. 164) ms; amplitude: a wave (69. 331 ±12. 355)μV, b wave (197.331 ± 16. 157) μV. Two weeks after injury implicit time of group B: a wave ( 18. 783 ± 1. 966 ) ms, b wave ( 52. 322 ± 4. 784) ms, amplitude: a wave ( 57. 562 ± 8. 217 ) μV, b wave ( 159. 569 ± 17. 859) μV. After four weeks feeding implicit time of group C: a wave ( 15. 115 ± 0. 940) ms, b wave (43. 242 ± 4. 662) ms, amplitude: a wave ( 72. 812 ± 4. 403 ) μV, b wave ( 207. 815 ± 14. 373 ) μV.Two weeks after injury implicit time of group C: a wave ( 15.957 ±2. 154) ms, b wave (44. 081 ±9. 506)ms, amplitude: a wave ( 66. 804 ± 8. 755 ) μV, b wave ( 186. 271 ± 29. 349 ) μV. These three groups had statistical significance in maximal combined reaction ERG( implicit time of a wave: fed 4 weeks F =6. 057,P ＜ 0. 05; two weeks after injury F = 13. 296, P ＜ 0. 05. Implicit time of b wave: fed 4 weeks F = 9. 949,P ＜ 0. 05; two weeks after injury F = 11. 145,P ＜ 0. 05. Amplitude of a wave: fed 4 weeks F = 8. 468, P ＜0. 05; two weeks after injury F = 4. 844, P ＜ 0. 05. Amplitude of b wave: fed 4 weeks F = 70. 194, P ＜0. 05; two weeks after injury F = 62. 161, P ＜ 0. 05). The total amplitudes of Ops( Σ Ops = OPs1 + OPs2 +OPs3 ) of them had statistical significance( fed 4 weeks F = 17. 482, P ＜ 0. 05; two weeks after injury F =11. 748 ,P ＜0. 05). By LSD test we found that before injury the amplitude of a wave and b wave in group B and C in maximal combined reaction ERG were significantly lower than those of group A which was fed by VU for 4 weeks (the a wave and b wave of group B compared to A: P =0. 003,0. 000; the a wave and b wave of group C compared to A: P =0. 001,0. 000). After light-induced injury, the implicit time of all the groups was increased and amplitude was decreased. But after the injury time of 1 day,1 week and 2 weeks, the amplitude of b wave of group A was respectively (229. 743 ± 11. 978 ) μV, (212. 785 ± 21. 021 ) μV,( 245. 887 ± 11. 797 ) μV, which was significantly higher than group B in the same period(P = 0. 000). With the accumulation of VU the ERG of group C was improving. Two weeks after injury the implicit time of b wave in group C was (44. 081 ±9. 506)ms and the amplitude was ( 186. 271 ±29. 349)μV. Compared with group B the former was decreased and the latter was increased significantly ( implicit time: P = 0. 008;amplitude: P = 0. 007). (2) Group D and E were normal in histology and lagyers were in order. While group B got disordered. Group A and C were injured slightly. The thickness of ONL among all groups had statistical significance( F = 330. 506, P ＜ 0. 05 ). ( 3 ) There was statistical significance among all groups in AI ( F =230. 126,P ＜0.05). AI of group B was (10.960±1.534)% and was higher than others'(P =0.000). AI of group D was (1. 817 ± 0. 203 ) % and lower than group E(P=0.000). Conclusions Vaccinium uliginosum can decrease retinal cell apoptosis and reduce photochemical damage to retinal tissue. In addition VU is able to promote retinal repairing after light damage.
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