中华医学杂志
中華醫學雜誌
중화의학잡지
National Medical Journal of China
2009年
19期
1309-1312
,共4页
奚才华%胡锦%王慧博%张舒羽%卢大儒
奚纔華%鬍錦%王慧博%張舒羽%盧大儒
해재화%호금%왕혜박%장서우%로대유
神经胶质瘤%跨损伤修复
神經膠質瘤%跨損傷脩複
신경효질류%과손상수복
Glioma%Translesion synthesis(TLS)
目的 研究DNA跨损伤修复基因Rev1、Pol ζ(Rev3和Rev7)、Pol η、Pol ι、和Pol κ在脑胶质瘤中的表达,探讨这些基因的表达水平与脑胶质瘤发生、发展的关系.方法 利用SYBR Green实时定量PCR技术检测DNA跨损伤修复基因在85例原发脑胶质瘤病人和14例正常脑组织中的mRNA表达情况.结果 与正常脑组织相比,Rev1基因在Ⅳ级胶质瘤中表达上调(ΔCt为5.55±1.12,P＜0.05);Rev3基因在Ⅱ级、Ⅲ级、Ⅳ级胶质瘤中表达上调(ΔCt分别为5.46±1.35、5.13±1.53和6.14±1.10,均P＜0.05);Rev7基因在Ⅱ级、Ⅲ级、Ⅳ级胶质瘤中表达上调(ΔCt分别为5.88±0.82、5.41±1.43和5.56±0.96,均P＜0.05);Pol η基因在Ⅱ级、Ⅲ级、Ⅳ级胶质瘤中表达上调(ΔCt分别为5.97±1.15、5.83±1.03和6.34±1.01,均P＜0.05);Pol ι基因在Ⅱ～Ⅳ级胶质瘤中表达上调(ΔCt分别为6.67±1.17、6.33±1.39和7.03±1.06,均P＜0.05);Pol κ基因在Ⅳ级胶质瘤中表达上调(ΔCt为5.25±1.58,P＜0.05).Spearman秩相关分析表明,跨损伤修复基因Rev1、Rev3、Pol η、Pol ι和Pol κ的表达量与肿瘤病理学分级呈正相关(均P＜0.01).结论 DNA跨损伤修复基因可能与胶质瘤的发生、进展相关.
目的 研究DNA跨損傷脩複基因Rev1、Pol ζ(Rev3和Rev7)、Pol η、Pol ι、和Pol κ在腦膠質瘤中的錶達,探討這些基因的錶達水平與腦膠質瘤髮生、髮展的關繫.方法 利用SYBR Green實時定量PCR技術檢測DNA跨損傷脩複基因在85例原髮腦膠質瘤病人和14例正常腦組織中的mRNA錶達情況.結果 與正常腦組織相比,Rev1基因在Ⅳ級膠質瘤中錶達上調(ΔCt為5.55±1.12,P＜0.05);Rev3基因在Ⅱ級、Ⅲ級、Ⅳ級膠質瘤中錶達上調(ΔCt分彆為5.46±1.35、5.13±1.53和6.14±1.10,均P＜0.05);Rev7基因在Ⅱ級、Ⅲ級、Ⅳ級膠質瘤中錶達上調(ΔCt分彆為5.88±0.82、5.41±1.43和5.56±0.96,均P＜0.05);Pol η基因在Ⅱ級、Ⅲ級、Ⅳ級膠質瘤中錶達上調(ΔCt分彆為5.97±1.15、5.83±1.03和6.34±1.01,均P＜0.05);Pol ι基因在Ⅱ～Ⅳ級膠質瘤中錶達上調(ΔCt分彆為6.67±1.17、6.33±1.39和7.03±1.06,均P＜0.05);Pol κ基因在Ⅳ級膠質瘤中錶達上調(ΔCt為5.25±1.58,P＜0.05).Spearman秩相關分析錶明,跨損傷脩複基因Rev1、Rev3、Pol η、Pol ι和Pol κ的錶達量與腫瘤病理學分級呈正相關(均P＜0.01).結論 DNA跨損傷脩複基因可能與膠質瘤的髮生、進展相關.
목적 연구DNA과손상수복기인Rev1、Pol ζ(Rev3화Rev7)、Pol η、Pol ι、화Pol κ재뇌효질류중적표체,탐토저사기인적표체수평여뇌효질류발생、발전적관계.방법 이용SYBR Green실시정량PCR기술검측DNA과손상수복기인재85례원발뇌효질류병인화14례정상뇌조직중적mRNA표체정황.결과 여정상뇌조직상비,Rev1기인재Ⅳ급효질류중표체상조(ΔCt위5.55±1.12,P＜0.05);Rev3기인재Ⅱ급、Ⅲ급、Ⅳ급효질류중표체상조(ΔCt분별위5.46±1.35、5.13±1.53화6.14±1.10,균P＜0.05);Rev7기인재Ⅱ급、Ⅲ급、Ⅳ급효질류중표체상조(ΔCt분별위5.88±0.82、5.41±1.43화5.56±0.96,균P＜0.05);Pol η기인재Ⅱ급、Ⅲ급、Ⅳ급효질류중표체상조(ΔCt분별위5.97±1.15、5.83±1.03화6.34±1.01,균P＜0.05);Pol ι기인재Ⅱ～Ⅳ급효질류중표체상조(ΔCt분별위6.67±1.17、6.33±1.39화7.03±1.06,균P＜0.05);Pol κ기인재Ⅳ급효질류중표체상조(ΔCt위5.25±1.58,P＜0.05).Spearman질상관분석표명,과손상수복기인Rev1、Rev3、Pol η、Pol ι화Pol κ적표체량여종류병이학분급정정상관(균P＜0.01).결론 DNA과손상수복기인가능여효질류적발생、진전상관.
Objective To detect the expressions of Rev1, Pol ζ, Pol η, Pol ι and Pol κ mRNA in human primary glioma tissues, and to analyze the relationship between the expression and the pathological grade.Methods We applied SYBR Green real-time PCR to investigate the mRNA expressions of the 6 genes in 85 primary glioma tissues and 14 normal brain tissues.Results The expressions of 4 genes including Rev3, Rev7, Pol η and Pol ι were significantly(P＜0.05)higher in glioma tissues in grade Ⅱ, Ⅲ and Ⅳ as compared to normal brain tissues. The expressions of Rev1 and Pol κ were significantly(P＜0.05)higher in grade Ⅳ glioma compared to the normal brain tissue. The positive correlation existed (P＜0.01) between the expression of all genes mRNA and the pathologic grade of the human glioma, except for Rev7.Conclusion The up-regulation of DNA translesion synthesis genes, including Rev1, Rev3, Rev7, Pol η, Pol ι and Pol κ, may be associated with pathogenesis of glioma.
