中南工业大学学报(自然科学版)
中南工業大學學報(自然科學版)
중남공업대학학보(자연과학판)
JOURNAL OF CENTRAL SOUTH UNIVERSITY OF TECHNOLGY(NATURAL SCIENCE)
2000年
6期
514-516
,共3页
羟自由基%茜素紫H2O2%C0SO4
羥自由基%茜素紫H2O2%C0SO4
간자유기%천소자H2O2%C0SO4
hydroxyl radical%alizarin violet%H2O2%CoSO4
研究了用茜素紫的氧化来显色测定Co2+-H2O2体系产生羟自由基(·OH)的方法,提出CO2+-H2O2-茜素紫分析新体系并用于羟自由基(·OH)的测定.本方法利用Co2+与H2O2反应,类似Fenton试剂产生羟自由基(·OH),并加入茜素紫显色剂,使茜素紫的颜色发生变化,采用紫外.可见分光光度计测定其△A值的变化,可间接测定羟自由基的含量,且灵敏度高.研究结果表明:在最大吸收波长560nm处,茜素紫的吸光度与·OH产生量成良好的线性关系;此外,抗氧化荆药物甘露醇、苯甲酸、硫脲与羟自由基清除率具有明显的量效关系,方法稳定性好。操作简便,测定快速,是一种简便的筛选抗羟自由基的清除剂和有效抗氧化剂药物的方法
研究瞭用茜素紫的氧化來顯色測定Co2+-H2O2體繫產生羥自由基(·OH)的方法,提齣CO2+-H2O2-茜素紫分析新體繫併用于羥自由基(·OH)的測定.本方法利用Co2+與H2O2反應,類似Fenton試劑產生羥自由基(·OH),併加入茜素紫顯色劑,使茜素紫的顏色髮生變化,採用紫外.可見分光光度計測定其△A值的變化,可間接測定羥自由基的含量,且靈敏度高.研究結果錶明:在最大吸收波長560nm處,茜素紫的吸光度與·OH產生量成良好的線性關繫;此外,抗氧化荊藥物甘露醇、苯甲痠、硫脲與羥自由基清除率具有明顯的量效關繫,方法穩定性好。操作簡便,測定快速,是一種簡便的篩選抗羥自由基的清除劑和有效抗氧化劑藥物的方法
연구료용천소자적양화래현색측정Co2+-H2O2체계산생간자유기(·OH)적방법,제출CO2+-H2O2-천소자분석신체계병용우간자유기(·OH)적측정.본방법이용Co2+여H2O2반응,유사Fenton시제산생간자유기(·OH),병가입천소자현색제,사천소자적안색발생변화,채용자외.가견분광광도계측정기△A치적변화,가간접측정간자유기적함량,차령민도고.연구결과표명:재최대흡수파장560nm처,천소자적흡광도여·OH산생량성량호적선성관계;차외,항양화형약물감로순、분갑산、류뇨여간자유기청제솔구유명현적량효관계,방법은정성호。조작간편,측정쾌속,시일충간편적사선항간자유기적청제제화유효항양화제약물적방법
A method of determining hydroxyl radical produced by Co2 + -H2 O2 system by chromogenic reaction of oxida-tion of alizarin violet has been introduced in this paper. The new analytical system of Co2 + -H2 O2-alizafin violet has beenfirst put forward and applied to determine Hydroxyl radicals. In this method, hydroxyl radicals are produced utilizing thereaction of Co2+ -H2 02 resembling the Fenton reaction, and at the same time, the chromogenic reagent of alizarin violet isadded to change the color of alizarin violet. The amount of hydroxyl radicals can be calculated indirectly by determiningthe variety of value of △A by UV absorption spectrum. The sensitivity of method is wholly higher than the classic ways ofFenton reaction. The optimum condition has been found by researching into the condition of determination. According tothe result, it is found that an apparent Iinear relation exists in the anti-oxidation radicals such as ascorbic acid, benzoicacid and hydroxyl radical. The results show that in the maximum wave of 560 nm, there is an excellent linear relationshipexisting between the absorption of alizarin violet and the production of · OH. This method has a merit of good stability,handy operation and quick determination so that it can be easily adopted by general laboratory and applied to filtrate anti-oxidation radicals as a handy elihmination reagent. It is also an effective method to filtrate medicament of anti-oxidation