麦类作物学报
麥類作物學報
맥류작물학보
JOURNAL OF TRITICEAE CROPS
2009年
6期
941-946
,共6页
小麦%醇溶-谷蛋白盒结合因子%RT-PCR%Real Time PCR
小麥%醇溶-穀蛋白盒結閤因子%RT-PCR%Real Time PCR
소맥%순용-곡단백합결합인자%RT-PCR%Real Time PCR
Triticum aestivum L.%WPBF%RT-PCR%Real Time PCR
为了给小麦品质改良的基因工程研究提供参考依据,以小麦醇溶-谷蛋白盒结合因子(Wheat Prolamin-Box Binding Factor, WPBF)基因为研究对象,从定性和定量两个方面,利用RT-PCR与Real Time PCR系统地研究了WPBF基因在小麦不同组织器官中以及在小麦胚乳整个发育过程中表达的时空特异性.结果表明,WPBF基因的表达是胚乳特异性的,表达跨胚乳的整个发育过程,在其表达的高峰阶段,表达强度为参照基因β-Actin的43%.
為瞭給小麥品質改良的基因工程研究提供參攷依據,以小麥醇溶-穀蛋白盒結閤因子(Wheat Prolamin-Box Binding Factor, WPBF)基因為研究對象,從定性和定量兩箇方麵,利用RT-PCR與Real Time PCR繫統地研究瞭WPBF基因在小麥不同組織器官中以及在小麥胚乳整箇髮育過程中錶達的時空特異性.結果錶明,WPBF基因的錶達是胚乳特異性的,錶達跨胚乳的整箇髮育過程,在其錶達的高峰階段,錶達彊度為參照基因β-Actin的43%.
위료급소맥품질개량적기인공정연구제공삼고의거,이소맥순용-곡단백합결합인자(Wheat Prolamin-Box Binding Factor, WPBF)기인위연구대상,종정성화정량량개방면,이용RT-PCR여Real Time PCR계통지연구료WPBF기인재소맥불동조직기관중이급재소맥배유정개발육과정중표체적시공특이성.결과표명,WPBF기인적표체시배유특이성적,표체과배유적정개발육과정,재기표체적고봉계단,표체강도위삼조기인β-Actin적43%.
To investigate the expression characteristics of wheat prolamin box binding factor (WPBF) in qualitative and quantitative aspects, we studied the temporal and spatial expression characteristics of WPBF gene in different tissues of wheat during the whole development stage of endosperm using RT-PCR and Real Time PCR methods. The results showed that the expression of WPBF was endosperm-specific; Its expression wasn't detected in roots, stems and leaves at seedling stage, and in roots, stems, leaves and young ears at booting stage. It expressed in the developing endosperm during the whole development stage of endosperm; its expression was weak at 0-4 days after pollination and increased rapidly subsequently, and reached the maximum level of expression at 20 days after pollination, which was 43% of the expression level of the reference gene that was used as β-Actin gene.
