光谱实验室
光譜實驗室
광보실험실
CHINESE JOURNAL OF SPECTROSCOPY LABORATORY
2010年
1期
37-42
,共6页
周在进%刘刚%任先培%张玉宾%罗庇荣
週在進%劉剛%任先培%張玉賓%囉庇榮
주재진%류강%임선배%장옥빈%라비영
蕈菌孢子%傅里叶变换红外光谱%相关分析
蕈菌孢子%傅裏葉變換紅外光譜%相關分析
심균포자%부리협변환홍외광보%상관분석
Spores of Mushrooms%Fourier Transform Infrared Spectroscopy%Correlation Analysis
傅里叶变换红外光谱法(FTIR)研究了9种野生荤菌的孢子,分析了主要吸收峰的归属,结果显示孢子的主要成分为脂类物质、蛋白质和多糖等.在1800-750cm~(-1)光谱范围内,不同属、不同种的野生荤菌孢子光谱显示了一定的差异.可以用来区分不同种类的孢子.对1800-1500cm~(-1)和1200-1000cm~(-1)范围的二阶导数光谱分别进行相关分析,9种孢子在1800-1500 cm~(-1)范围内的相关性相对较好,相关系数在0.843-0.998范围,表明它们含有的主要化学成分脂类物质、蛋白质等比较相似;在1200-1000cm~(-1)范围内相关性较差,相关系数最大为0.780,最小为0.144,表明多糖物质构型或含量具有较大差异.傅里叶变换红外光谱结合特征峰分析和相关分析极有可能用于鉴别蕈菌孢子.
傅裏葉變換紅外光譜法(FTIR)研究瞭9種野生葷菌的孢子,分析瞭主要吸收峰的歸屬,結果顯示孢子的主要成分為脂類物質、蛋白質和多糖等.在1800-750cm~(-1)光譜範圍內,不同屬、不同種的野生葷菌孢子光譜顯示瞭一定的差異.可以用來區分不同種類的孢子.對1800-1500cm~(-1)和1200-1000cm~(-1)範圍的二階導數光譜分彆進行相關分析,9種孢子在1800-1500 cm~(-1)範圍內的相關性相對較好,相關繫數在0.843-0.998範圍,錶明它們含有的主要化學成分脂類物質、蛋白質等比較相似;在1200-1000cm~(-1)範圍內相關性較差,相關繫數最大為0.780,最小為0.144,錶明多糖物質構型或含量具有較大差異.傅裏葉變換紅外光譜結閤特徵峰分析和相關分析極有可能用于鑒彆蕈菌孢子.
부리협변환홍외광보법(FTIR)연구료9충야생훈균적포자,분석료주요흡수봉적귀속,결과현시포자적주요성분위지류물질、단백질화다당등.재1800-750cm~(-1)광보범위내,불동속、불동충적야생훈균포자광보현시료일정적차이.가이용래구분불동충류적포자.대1800-1500cm~(-1)화1200-1000cm~(-1)범위적이계도수광보분별진행상관분석,9충포자재1800-1500 cm~(-1)범위내적상관성상대교호,상관계수재0.843-0.998범위,표명타문함유적주요화학성분지류물질、단백질등비교상사;재1200-1000cm~(-1)범위내상관성교차,상관계수최대위0.780,최소위0.144,표명다당물질구형혹함량구유교대차이.부리협변환홍외광보결합특정봉분석화상관분석겁유가능용우감별심균포자.
Fourier transform infrared spectroscopy(FTIR)was used to study spores of wild growing mushrooms.The infrared spectra of spores are mainly composed of the absorption of lipids,protein and polysaccharide.The spectra exhibit small differences in the range of 1800-750cm~(-1).The second derivative spectra in two regions of 1800-1500cm~(-1) and 1200-1000cm~(-1) were used to evaluate correlation coefficients between the spectra.The spores of different species of wild growing mushrooms have good correlation in the spectra range of 1800-1500cm~(-1),their correlation coefficients vary from 0.843 to 0.998,which indicate that the main chemical components of spores such as lipids and protein are very similar.The correlation coefficients vary from 0.144 to 0.780 in the range of 1200-1000cm~(-1),which indicate the great differences in the structure and contents of polysaccharide between different species spores.The FTIR spectra combined with correlation analysis might be used to identify spores of mushrooms rapidly and conveniently.