CAJ | 학술논문

[目的]为饲料中单端孢霉烯族真菌毒素的有效、快速检测提供理论支持.[方法]通过比较单端孢霉烯族毒素的检测方法,探讨了相关问题并预测了饲料中单端孢霉烯族毒素检测方法的发展方向.[结果]使用TLC检测脱氧雪腐镰刀菌烯醇的检测限可达20～300 μg/kg.在羟基衍生化后用气相色谱电子俘获法检测A类单端孢霉烯族毒素的检测限为10～50 μg/kg,衍生化或荧光酰化后检测B类单端孢霉烯族毒素的检测限可达20～50 μg/kg,甚至更低.利用HPLC,以甲醇-水或乙腈-水混合物为流动相检测单端孢霉烯族毒素,其检测限为100～500 μg/kg.利用LC-MS/MS检测单端孢霉烯族毒素的检测限可达0.8～10.0 ng/kg.[结论]随着技术的发展,将会有越来越多的优良分析检测方法用于检测饲料中的单端孢霉烯族毒素.
[목적]위사료중단단포매희족진균독소적유효、쾌속검측제공이론지지.[방법]통과비교단단포매희족독소적검측방법,탐토료상관문제병예측료사료중단단포매희족독소검측방법적발전방향.[결과]사용TLC검측탈양설부렴도균희순적검측한가체20～300 μg/kg.재간기연생화후용기상색보전자부획법검측A류단단포매희족독소적검측한위10～50 μg/kg,연생화혹형광선화후검측B류단단포매희족독소적검측한가체20～50 μg/kg,심지경저.이용HPLC,이갑순-수혹을정-수혼합물위류동상검측단단포매희족독소,기검측한위100～500 μg/kg.이용LC-MS/MS검측단단포매희족독소적검측한가체0.8～10.0 ng/kg.[결론]수착기술적발전,장회유월래월다적우량분석검측방법용우검측사료중적단단포매희족독소.
[Objective] The purpose was to supply theoretical support for the effective and rapid detection of trichothecene mycotoxins in feeds. [Method] Through comparing the detection methods of trichothecene mycotoxins, some problems existing in these methods were discussed and the developing direction of detection methods of trichothecene mycotoxins in feeds was predicted. [Result] The detection limit of TLC in detecting deoxynivalenol was as low as 20-300 μg/kg. The detection limit range of GC electron capture detection in detecting the hydroxyl-derivative of type-A trichothecene mycotoxins was 10-50 μg/kg and its detection limit in detecting the derivative and fluorescent acylate of type-B trichothecene mycotoxins was as low as 20-50 μg/kg, even lower. The detection limit range of HPLC in detecting trichothecene mycotoxins with methanol-water or acetonitrile-water mixture as mobile phase was 100-500 μg/kg. The detection limit of LC-MS/MS in detecting trichothecene mycotoxins was as low as 0.8-10.0 ng/kg. [Conclusion] As the technology was developed, there would be more and more good analysis and detection methods used in detecting trichothecene mycotoxins in feeds.