四川大学学报（医学版）
四川大學學報（醫學版）
사천대학학보（의학판）
JOURNAL OF SICHUAN UNIVERSITY(MEDICAL SCIENCE EDITION)
2010年
2期
276-279
,共4页
百草枯%NF-κB%炎症反应%炎症因子
百草枯%NF-κB%炎癥反應%炎癥因子
백초고%NF-κB%염증반응%염증인자
Paraquat%NF-κB%Inflammatory reaction%Inflammatory factor
目的 探讨百草枯处理后细胞内NF-κB的激活以及下游因子IL-6、IL-8的表达情况.方法 体外培养人脐静脉血管内皮细胞(HUVEC)建立百草枯处理模型.在半数死亡浓度的百草枯作用8 h、24 h、48 h后,利用免疫细胞化学方法检测细胞NF-κB P65蛋白活化及定位,利用RT-PCR扩增IL-6、IL-8,半定量分析其mRNA表达水平变化.结果 免疫组化显示正常细胞NF-κB P65定位于细胞胞浆,百草枯作用后开始出现细胞核转位,并随作用时间延长核转位数目明显增加;RT-PCR结果显示百草枯作用后NF-κB的下游产物IL-6、IL-8 mRNA表达水平均明显上调,并可持续到48 h.结论 百草枯处理后早期即可能出现NF-κB的激活,进而诱导了下游的重要炎性介质的合成,促发和推进炎症反应,说明百草枯中毒后早期即有炎症反应参与了组织的损伤.
目的 探討百草枯處理後細胞內NF-κB的激活以及下遊因子IL-6、IL-8的錶達情況.方法 體外培養人臍靜脈血管內皮細胞(HUVEC)建立百草枯處理模型.在半數死亡濃度的百草枯作用8 h、24 h、48 h後,利用免疫細胞化學方法檢測細胞NF-κB P65蛋白活化及定位,利用RT-PCR擴增IL-6、IL-8,半定量分析其mRNA錶達水平變化.結果 免疫組化顯示正常細胞NF-κB P65定位于細胞胞漿,百草枯作用後開始齣現細胞覈轉位,併隨作用時間延長覈轉位數目明顯增加;RT-PCR結果顯示百草枯作用後NF-κB的下遊產物IL-6、IL-8 mRNA錶達水平均明顯上調,併可持續到48 h.結論 百草枯處理後早期即可能齣現NF-κB的激活,進而誘導瞭下遊的重要炎性介質的閤成,促髮和推進炎癥反應,說明百草枯中毒後早期即有炎癥反應參與瞭組織的損傷.
목적 탐토백초고처리후세포내NF-κB적격활이급하유인자IL-6、IL-8적표체정황.방법 체외배양인제정맥혈관내피세포(HUVEC)건립백초고처리모형.재반수사망농도적백초고작용8 h、24 h、48 h후,이용면역세포화학방법검측세포NF-κB P65단백활화급정위,이용RT-PCR확증IL-6、IL-8,반정량분석기mRNA표체수평변화.결과 면역조화현시정상세포NF-κB P65정위우세포포장,백초고작용후개시출현세포핵전위,병수작용시간연장핵전위수목명현증가;RT-PCR결과현시백초고작용후NF-κB적하유산물IL-6、IL-8 mRNA표체수평균명현상조,병가지속도48 h.결론 백초고처리후조기즉가능출현NF-κB적격활,진이유도료하유적중요염성개질적합성,촉발화추진염증반응,설명백초고중독후조기즉유염증반응삼여료조직적손상.
Objective To investigate the expression of NF-κB and its downstream products in the human umbilical vascular endothelial cells (HUVEC) treated with paraquate. Methods The HUVEC were cultured in vitro. The activation and location of NF-κB P65 protein were detected by immunocytochemical method at 8 h, 24 h and 48 h after treatment with paraquat. The mRNA expression of IL-6 and IL-8 were detected by RT-PCR. Results Immunocytochemical analyses revealed that the activity subunit of NF-κB P65 was located in cytoplasm of normal HUVEC. When the cells were treated with paraquat, positive staining in nuclear was observed. The sum of positive nuclear got more as the paraquat treated time increased. The RT-PCR verified the mRNA expression of IL-6 and IL-8 was up-regulated until 48 h after treatment with paraquat. Conlusion NF-κB could be activated at the early phase of paraquat poisoning. The activated NF-κB will induce the synthesis of inflammatory factors such as IL-6, IL-8 and then induce the inflammatory reaction. The inflammatory reaction have participated the tissue damage at the early phase of paraquat poisioning.
