CAJ | 학술논문

目的观察慢性胃炎脾胃湿热证患者胃黏膜核因子-κB(nuclear factor κB,NF-κB)mRNA、热休克蛋白70(heat shock protein70,HSP70)mRNA转录水平表达,探讨其与脾胃湿热证发生及幽门螺杆菌(Helico-bacter pylori,HP)感染的关系.方法收集慢性胃炎患者51例(包括脾胃湿热证36例,脾气虚证15例),另设健康人8名作为对照.胃镜下取胃窦黏膜,采用苏木精伊红(HE)染色检测胃黏膜炎症,快速尿素酶和甲苯胺蓝法检测Hp感染,荧光定量PCR检测各组胃黏膜NF-κB mRNA和HSP70 mRNA表达水平.结果 HSP70 mRNA表达:脾胃湿热证组高于健康对照组,差异有统计学意义(P<0.05);脾胃湿热证Hp阴性组与健康对照组比较差异有统计学意义(P<0.05),较健康对照组表达高;脾胃湿热Hp阳性与Hp阴性组比较差异无统计学意义(P>0.05).NF-κB mRNA表达:脾胃湿热证组表达高于脾气虚证组和健康对照组,差异有统计学意义(P<0.05);脾胃湿热证Hp阳性组与Hp阴性组比较差异无统计学意义(P>0.05).脾胃湿热证组及脾胃湿热Hp阴性者HSPTO mRNA与NF-κB mRNA表达水平呈正相关.结论 NF-κB和HSP70在一定程度上体现了机体邪气和正气的力量,慢性胃炎脾胃湿热证两者的高表达可能部分体现了"邪正交争"剧烈的状态;而代表正气和邪气力量的HSP70和NF-κB在交争过程中均可能会受到不同程度的影响.
목적 관찰만성위염비위습열증환자위점막핵인자-κB(nuclear factor κB,NF-κB)mRNA、열휴극단백70(heat shock protein70,HSP70)mRNA전록수평표체,탐토기여비위습열증발생급유문라간균(Helico-bacter pylori,HP)감염적관계.방법 수집만성위염환자51례(포괄비위습열증36례,비기허증15례),령설건강인8명작위대조.위경하취위두점막,채용소목정이홍(HE)염색검측위점막염증,쾌속뇨소매화갑분알람법검측Hp감염,형광정량PCR검측각조위점막NF-κB mRNA화HSP70 mRNA표체수평.결과 HSP70 mRNA표체:비위습열증조고우건강대조조,차이유통계학의의(P<0.05);비위습열증Hp음성조여건강대조조비교차이유통계학의의(P<0.05),교건강대조조표체고;비위습열Hp양성여Hp음성조비교차이무통계학의의(P>0.05).NF-κB mRNA표체:비위습열증조표체고우비기허증조화건강대조조,차이유통계학의의(P<0.05);비위습열증Hp양성조여Hp음성조비교차이무통계학의의(P>0.05).비위습열증조급비위습열Hp음성자HSPTO mRNA여NF-κB mRNA표체수평정정상관.결론 NF-κB화HSP70재일정정도상체현료궤체사기화정기적역량,만성위염비위습열증량자적고표체가능부분체현료"사정교쟁"극렬적상태;이대표정기화사기역량적HSP70화NF-κB재교쟁과정중균가능회수도불동정도적영향.
Objective To explore the relationship between the genesis of Pi-Wei damp-heat syndrome (PDS) and the Helicobacter pylori (HP) infection in patients with chronic gastritis (CG) by observing the levels of nuclear factor κB (NF-κB) mRNA and heat shock protein 70 (HSP70) expressions. Methods Gastric mucous membrane tissues collected through gastroscopy from gastric antrum of 51 CG patients, 36 of PDS type (CG-PDS) and 15 of Pi-qi deficiency syndrome (CG-non-PDS) type, and 8 healthy persons (as control) were examined to diagnose the inflammation level with HE stain and to detect the existence of HP infection by rapid urease test and methylthioninium chloride stain. The mRNA expressions of NF-κB and HSP70 in the tissues were determined quantitatively with FQ-PCR as well. Results Patients of CG-PDS showed higher level of HSP70 mRNA expression than in the control; and level of NF-κB mRNA expression was higher than that in the control and CG-non-PDS (all P<0.05); but both expressions were insignificantly different in CG-PDS patients with positive or negative HP infection (P>0.05). Positive correlation of the two expressions was shown in CG-PDS with negative HP infection. Conclusion NF-κB and HSP70 may partially embody, in some extent, the power of vital qi and evil qi in the organism; the over-expressed NF-κB and HSP70 may indicate the severe fighting between evil qi and vital qi, and both would be influenced to a certain degree in the fighting process.