中华胰腺病杂志
中華胰腺病雜誌
중화이선병잡지
CHINESE JOURNAL OF PANCREATOLOGY
2011年
1期
37-39
,共3页
马建霞%司佩任%吴洪玉%王洛伟%潘雪%高军%李兆申%金震东
馬建霞%司珮任%吳洪玉%王洛偉%潘雪%高軍%李兆申%金震東
마건하%사패임%오홍옥%왕락위%반설%고군%리조신%금진동
胰腺肿瘤%模型,动物%内镜超声检查
胰腺腫瘤%模型,動物%內鏡超聲檢查
이선종류%모형,동물%내경초성검사
Pancreatic neoplasms%Model,animal%Endoscopy Ultrasonography
目的 建立裸小鼠人胰腺癌原位种植瘤模型,探索监测种植瘤生长的方法.方法 将对数生长期的人胰腺癌细胞株SW1990制备成细胞悬液,原位注射于Balb/c-nu裸小鼠胰腺尾部包膜下,利用高频内镜超声(EUS)探头体表观察肿瘤结节的生长及声像图像.结果 20只裸小鼠均接种成功,1只裸鼠于接种后25 d时死亡.接种后14 d,EUS检查的瘤体大小为(8.09±2.61)mm3,肿瘤结节呈均质低回声,边界清楚,周边有包膜及声晕,形态规则,30%的肿瘤结节周边可见低速环绕彩色血流信号;接种后28 d,瘤体增大至(12.40±3.51)mm3,70%的肿瘤结节呈不规则形,部分为分叶状,肿块呈低回声,不均质,未见液化坏死区,70%的肿瘤结节周边可见低速环绕彩色血流信号.结论 原位注射法是建立裸小鼠人胰腺癌原位种植瘤模型较理想的方法,操作简便,成瘤率高;高频内镜超声显像是可靠的监测胰腺原位种植瘤的手段.
目的 建立裸小鼠人胰腺癌原位種植瘤模型,探索鑑測種植瘤生長的方法.方法 將對數生長期的人胰腺癌細胞株SW1990製備成細胞懸液,原位註射于Balb/c-nu裸小鼠胰腺尾部包膜下,利用高頻內鏡超聲(EUS)探頭體錶觀察腫瘤結節的生長及聲像圖像.結果 20隻裸小鼠均接種成功,1隻裸鼠于接種後25 d時死亡.接種後14 d,EUS檢查的瘤體大小為(8.09±2.61)mm3,腫瘤結節呈均質低迴聲,邊界清楚,週邊有包膜及聲暈,形態規則,30%的腫瘤結節週邊可見低速環繞綵色血流信號;接種後28 d,瘤體增大至(12.40±3.51)mm3,70%的腫瘤結節呈不規則形,部分為分葉狀,腫塊呈低迴聲,不均質,未見液化壞死區,70%的腫瘤結節週邊可見低速環繞綵色血流信號.結論 原位註射法是建立裸小鼠人胰腺癌原位種植瘤模型較理想的方法,操作簡便,成瘤率高;高頻內鏡超聲顯像是可靠的鑑測胰腺原位種植瘤的手段.
목적 건립라소서인이선암원위충식류모형,탐색감측충식류생장적방법.방법 장대수생장기적인이선암세포주SW1990제비성세포현액,원위주사우Balb/c-nu라소서이선미부포막하,이용고빈내경초성(EUS)탐두체표관찰종류결절적생장급성상도상.결과 20지라소서균접충성공,1지라서우접충후25 d시사망.접충후14 d,EUS검사적류체대소위(8.09±2.61)mm3,종류결절정균질저회성,변계청초,주변유포막급성훈,형태규칙,30%적종류결절주변가견저속배요채색혈류신호;접충후28 d,류체증대지(12.40±3.51)mm3,70%적종류결절정불규칙형,부분위분협상,종괴정저회성,불균질,미견액화배사구,70%적종류결절주변가견저속배요채색혈류신호.결론 원위주사법시건립라소서인이선암원위충식류모형교이상적방법,조작간편,성류솔고;고빈내경초성현상시가고적감측이선원위충식류적수단.
Objective To establish the orthotopic human pancreatic cancer model in mice and study the method to detect the tumor growth. Methods Human pancreatic cancer cell lines SW1990 in logarithmic phase was made into cell suspension and in situ injected into the envelope of pancreatic tail of BALB/c nude mice. High-frequency endoscopy ultrasound was used to observe the growth of tumor mass and its imaging characteristics were studied. Results 20 nude mice were successfully implanted, and 1 died 25 h after implantation. 14 days after implantation, the tumor of pancreatic cancer on EUS was (8.09 ± 2.61) mm3, the tumor appeared as homogeneous hypoechoic mass with clear boundary, and envelope as well as sound halo was present, and the shape was regular; there was low speed circular vasculature signal in around 30% of tumor.The tumor size increased to (12.40 ± 3.51)mm3, and the shape of 70% tumor was irregular, and some appeared as lobulated, and the tumor appeared as heterogeneous hypoechoic mass, no necrosis or liquefaction was found 28 days after implantation. There was low speed circular vasculature signal in around 70% tumors.Conclusions The orthotopic pancreatic cancer model in nude mouse can be established by in situ injection and this method is relatively ideal because it is simple and effective. The high frequency probe of endoscopic ultrasonograph is a reliable method for monitoring implanted pancreatic cancer.
