CAJ | 학술논문

目的研究间质细胞衍生因子1(SDF-1)及其受体CXCR4在浸润性乳腺癌中的表达,并分析其与浸润性乳腺癌相关临床病理指标及淋巴结转移之间的关系.方法采用免疫组织化学LSAB方法检测SDF-1/CXCR4在120例浸润性乳腺癌中的表达情况;采用地高辛标记的寡核苷酸探针进行原位杂交以检测趋化因子SDF-1在肿瘤环境中表达的部位及来源.结果 (1)SDF-1主要表达于肿瘤细胞的胞质和胞膜;SDF-1的胞质表达在淋巴结阳性组高于阴性组(P=0.033),且其表达程度与淋巴结受累数目、病理学分期、组织学分级、肿瘤大小及ER表达等指标呈正相关(P<0.05);(2)罕见SDF-1 mRNA表达的脉管内皮却可见SDF-1蛋白的表达,其表达程度与肿瘤胞质SDF-1着色正相关(P<0.01);且淋巴管内皮SDF-1的着色与淋巴结转移程度为正相关(P=0.005);血管内皮SDF-1的着色与肿瘤环境中的淋巴细胞浸润正相关(P<0.01),且同时伴有较多淋巴细胞浸润及SDF-1血管内皮着色阳性的病例,其淋巴结的转移程度分别高于仅有上述条件之一或二者均不具备的各组病例(P<0.05);(3)CXCR4也主要表达于肿瘤细胞的胞质和胞核;CXCR4的胞质表达在淋巴结阳性组高于阴性组(P<0.05),且其表达程度与淋巴结受累数目、病理学分期、组织学分级、肿瘤大小及HER2表达等呈正相关(P=0.005),而胞核的表达仅与PR的表达情况呈正相关(P<0.01);(4)瘤细胞质CXCR4与SDF-1的表达呈正相关(P=0.001).结论浸润性乳腺癌肿瘤细胞SDF-1和CXCR4的表达与多项临床病理指标,尤其是淋巴结转移率及转移程度有关,可作为预测乳腺癌淋巴结转移及预后的免疫病理学指标,同时应注意肿瘤微环境中SDF-1的多个来源及定位的不同意义.
목적 연구간질세포연생인자1(SDF-1)급기수체CXCR4재침윤성유선암중적표체,병분석기여침윤성유선암상관림상병리지표급림파결전이지간적관계.방법 채용면역조직화학LSAB방법검측SDF-1/CXCR4재120례침윤성유선암중적표체정황;채용지고신표기적과핵감산탐침진행원위잡교이검측추화인자SDF-1재종류배경중표체적부위급래원.결과 (1)SDF-1주요표체우종류세포적포질화포막;SDF-1적포질표체재림파결양성조고우음성조(P=0.033),차기표체정도여림파결수루수목、병이학분기、조직학분급、종류대소급ER표체등지표정정상관(P<0.05);(2)한견SDF-1 mRNA표체적맥관내피각가견SDF-1단백적표체,기표체정도여종류포질SDF-1착색정상관(P<0.01);차림파관내피SDF-1적착색여림파결전이정도위정상관(P=0.005);혈관내피SDF-1적착색여종류배경중적림파세포침윤정상관(P<0.01),차동시반유교다림파세포침윤급SDF-1혈관내피착색양성적병례,기림파결적전이정도분별고우부유상술조건지일혹이자균불구비적각조병례(P<0.05);(3)CXCR4야주요표체우종류세포적포질화포핵;CXCR4적포질표체재림파결양성조고우음성조(P<0.05),차기표체정도여림파결수루수목、병이학분기、조직학분급、종류대소급HER2표체등정정상관(P=0.005),이포핵적표체부여PR적표체정황정정상관(P<0.01);(4)류세포질CXCR4여SDF-1적표체정정상관(P=0.001).결론 침윤성유선암종류세포SDF-1화CXCR4적표체여다항림상병리지표,우기시림파결전이솔급전이정도유관,가작위예측유선암림파결전이급예후적면역병이학지표,동시응주의종류미배경중SDF-1적다개래원급정위적불동의의.
Objective To study the expression of stromal cell derived factor 1 (SDF-1)/CXCR4 and their association with clinicopathologic features and lymph node metastasis in invasive breast carcinoma.Methods The expression of SDF-1 was studied by immunohistoehemistry and in-situ hybridization.Immunohistochemical study for CXCR4 was also performed. The correlation with various clinieopathologic parameters was analyzed. Results (1) SDF-1 was mainly expressed in tumor cells and the level of its expression (both membranous and cytoplasmic) in lymph node-positive group was higher than that in lymph node-negative group (P=0.033). Only cytoplasmic expression correlated with the number of positive lymphnode involved by metastasis, TNM tumor stage, histologic grade, tumor dimension and estrogen receptor status (P<0.05). (2) SDF-1 protein was also detected in the endothelial cells, although its mRNA was rarely detected. SDF-1 staining in lymphatics was associated with positive lymph node (P=0.005) and SDF-1 staining in blood vessels correlated with stromal lymphocytic reaction (P=0.001). The extent of nodal involvement was higher in the group with positive SDF-1 staining in blood vessels and with prominent lymphocytic reaction than that in other groups with one or neither of the two features (P<0.05). (3) On the other hand, CXCR4 was mainly expressed in tumor cells (both nuclear and cytoplasmic) ; and the level of its expression in lymph node-positive group was higher than that in lymph node-negative group (P=0.005). Only cytoplasmic expression correlated with the number of positive lymph node involved by metastasis, TNM tumor stage, histologic grade, tumor dimension and HER2 status (P<0.05). The nuclear expression of CXCR4 was only correlated with progesterone receptor status (P<0.01). The cytoplasmic expression CXCR4 also positively correlated with SDF-1 expression (P=0.001). Conclusions SDF-1 and CXCR4 can serve as biomarkers for diagnosis and prediction of lymph node metastasis, as well as potential therapeutic targets in invasive breast carcinoma. The difference in localization and staining patterns may also carry different significance.