中华麻醉学杂志
中華痳醉學雜誌
중화마취학잡지
CHINESE JOURNAL OF ANESTHESIOLOGY
2011年
4期
497-499
,共3页
田伟千%崔苏扬%姚凤珍%李宝贵
田偉韆%崔囌颺%姚鳳珍%李寶貴
전위천%최소양%요봉진%리보귀
电针%再灌注损伤%能量代谢%脑
電針%再灌註損傷%能量代謝%腦
전침%재관주손상%능량대사%뇌
Electroacupuncture%Reperfusion injury%Energy metabolism%Brain
目的 探讨不同强度电针刺激对脑缺血再灌注大鼠脑能量代谢的影响.方法清洁级雄性SD大鼠40只,体重200~230 g,采用随机数字表法,将大鼠随机分为5组(n=8):假手术组(S组)、脑缺血再灌注组(I/R组)、脑缺血再灌注+5 mA电针组(I/R+E1组)、脑缺血再灌注+3 mA电针组(I/R+E2组)和脑缺血再灌注+1 mA电针组(I/R+E3组).采用四血管阻断法制备脑缺血再灌注模型.I/R+E1组、I/R+E2组和I/R+E3组分别于模型制备后1、12 h实施电针刺激,20 min/次,电流强度分别为5、3和1 mA.再灌注24 h时,测定脑组织琥珀酸脱氢酶(SDH)、LDH和Na+-K+-ATP酶的活性.结果 与S组比较,I/R组脑组织LDH活性升高,Na+-K+-ATP酶活性降低(P<0.05),SDH活性差异无统计学意义(P>0.05),I/R+E1组SDH活性升高(P<0.05);与I/R组比较,I/R+E1组脑组织LDH活性降低,SDH和Na+-K+-ATP酶活性升高,I/R+E2组和I/R+E3组LDH活性降低(P<0.05或0.01),SDH和Na+-K+-ATP酶活性差异无统计学意义(P>0.05);与I/R+E1组比较,I/R+E3组脑组织SDH活性降低(P<0.05),I/R+E2组差异无统计学意义(P>0.05);与I/R+E2组比较,I/R+E3组脑组织SDH活性降低(P<0.05).结论 电针刺激可改善大鼠脑缺血再灌注时的脑能量代谢,且与刺激强度有关,该作用可能是其减轻脑缺血再灌注损伤的机制.
目的 探討不同彊度電針刺激對腦缺血再灌註大鼠腦能量代謝的影響.方法清潔級雄性SD大鼠40隻,體重200~230 g,採用隨機數字錶法,將大鼠隨機分為5組(n=8):假手術組(S組)、腦缺血再灌註組(I/R組)、腦缺血再灌註+5 mA電針組(I/R+E1組)、腦缺血再灌註+3 mA電針組(I/R+E2組)和腦缺血再灌註+1 mA電針組(I/R+E3組).採用四血管阻斷法製備腦缺血再灌註模型.I/R+E1組、I/R+E2組和I/R+E3組分彆于模型製備後1、12 h實施電針刺激,20 min/次,電流彊度分彆為5、3和1 mA.再灌註24 h時,測定腦組織琥珀痠脫氫酶(SDH)、LDH和Na+-K+-ATP酶的活性.結果 與S組比較,I/R組腦組織LDH活性升高,Na+-K+-ATP酶活性降低(P<0.05),SDH活性差異無統計學意義(P>0.05),I/R+E1組SDH活性升高(P<0.05);與I/R組比較,I/R+E1組腦組織LDH活性降低,SDH和Na+-K+-ATP酶活性升高,I/R+E2組和I/R+E3組LDH活性降低(P<0.05或0.01),SDH和Na+-K+-ATP酶活性差異無統計學意義(P>0.05);與I/R+E1組比較,I/R+E3組腦組織SDH活性降低(P<0.05),I/R+E2組差異無統計學意義(P>0.05);與I/R+E2組比較,I/R+E3組腦組織SDH活性降低(P<0.05).結論 電針刺激可改善大鼠腦缺血再灌註時的腦能量代謝,且與刺激彊度有關,該作用可能是其減輕腦缺血再灌註損傷的機製.
목적 탐토불동강도전침자격대뇌결혈재관주대서뇌능량대사적영향.방법청길급웅성SD대서40지,체중200~230 g,채용수궤수자표법,장대서수궤분위5조(n=8):가수술조(S조)、뇌결혈재관주조(I/R조)、뇌결혈재관주+5 mA전침조(I/R+E1조)、뇌결혈재관주+3 mA전침조(I/R+E2조)화뇌결혈재관주+1 mA전침조(I/R+E3조).채용사혈관조단법제비뇌결혈재관주모형.I/R+E1조、I/R+E2조화I/R+E3조분별우모형제비후1、12 h실시전침자격,20 min/차,전류강도분별위5、3화1 mA.재관주24 h시,측정뇌조직호박산탈경매(SDH)、LDH화Na+-K+-ATP매적활성.결과 여S조비교,I/R조뇌조직LDH활성승고,Na+-K+-ATP매활성강저(P<0.05),SDH활성차이무통계학의의(P>0.05),I/R+E1조SDH활성승고(P<0.05);여I/R조비교,I/R+E1조뇌조직LDH활성강저,SDH화Na+-K+-ATP매활성승고,I/R+E2조화I/R+E3조LDH활성강저(P<0.05혹0.01),SDH화Na+-K+-ATP매활성차이무통계학의의(P>0.05);여I/R+E1조비교,I/R+E3조뇌조직SDH활성강저(P<0.05),I/R+E2조차이무통계학의의(P>0.05);여I/R+E2조비교,I/R+E3조뇌조직SDH활성강저(P<0.05).결론 전침자격가개선대서뇌결혈재관주시적뇌능량대사,차여자격강도유관,해작용가능시기감경뇌결혈재관주손상적궤제.
Objective To investigate the effects of electroacupuncture (EA) of different intensities on cerebral energy metabolism in a rat model of global cerebral ischemia-reperfusion (I/R) . Methods Forty male SD rats weighing 200-230 g were randomized into 5 groups ( n = 8 each) : group A sham operation; group B global cerebral I/R and C, D, E groups cerebral I/R+ 5, 3, 1 mA EA. Global cerebral I/R was induced by 4-vessel occlusion technique. Bilateral vertebral arteries were permanently occluded by cauterization.Bilateral common carotid arteries were clamped. When the bilateral pupils were completely dilated, the arteries were unclamped. Baihui,Mingmen and Zusanli were electrically stimulated with 5,3,1 mA (30-50 Hz) for 20 min at 1 h of reperfusion in C, D, E groups. The EA was repeated at 12 h of reperfusion. The animals were sacrificed at 24 h of reperfusion.The activities of Na+ -K+ -ATPase, succinodehydrogenase (SDH) and lactic dehydrogenase(LDH) in brain tissue were measured.Results Cerebral I/R significantly increased LDH activity and decreased Na+ -K+ -ATPase activity in group B as compared with group A. EA with 5 mA significantly decreased LDH activity and increased SDH and Na+ -K+ -ATPase activities in group C compared with group B. Conclusion EA can improve the cerebral energy metabolism in a rat model of global cerebral I/R and it is related to the intensity, which may be the mechanism by which EA reduces the global cerebral I/R injury.