中华劳动卫生职业病杂志
中華勞動衛生職業病雜誌
중화노동위생직업병잡지
CHINESE JOURNAL OF INDUSTRIAL HYGIENE AND OCCUPATIONAL DISEASES
2008年
6期
327-331
,共5页
陈传芳%崔元波%乐加昌%霍小林%宋涛
陳傳芳%崔元波%樂加昌%霍小林%宋濤
진전방%최원파%악가창%곽소림%송도
电磁场%H(+)转运ATP合酶%细菌蛋白质类
電磁場%H(+)轉運ATP閤酶%細菌蛋白質類
전자장%H(+)전운ATP합매%세균단백질류
Electromagnetic fields%H(+)-Transporting ATP synthase%Bacterial proteins
目的 研究极低频正弦磁场对F0F1-ATPases活力的影响及其机制.方法 强度为0.1～0.5 mT、频率为4.7～96.0 Hz的磁场分别处理从光合细菌(Rhodospirillum rubrum)中提取的细菌色素体(chromatophores)外膜上的F0F1-ATPases.结果 0.5 mT,4.7、12.0、60.0、72.0、84.0、96.0 Hz磁场对F0F1-ATPases水解起促进作用,0.5 mT、24.0 Hz磁场对F0F1-ATPases水解起抑制作用,差异均有统计学意义(P＜0.05);0.3 mT,4.7、24.0、60.0 Hz的磁场对F0F1-ATPases仍有明显影响,但0.1 mT的磁场则对F0F1-ATPases无明显影响.0.5 mT,24.0、60.0 Hz的磁场对二环己基碳二亚胺(DCCD)抑制的F0F1-ATPases仍分别有明显地抑制和促进酶活力的影响,差异有统计学意义(P＜0.05).结论 影响F0F1-ATPases活力的极低频磁场具有频率窗口,强度阈值在0.1～0.3 mT之间;F0F1-ATPases,特别是F1是磁场作用的一个重要的位点.
目的 研究極低頻正絃磁場對F0F1-ATPases活力的影響及其機製.方法 彊度為0.1～0.5 mT、頻率為4.7～96.0 Hz的磁場分彆處理從光閤細菌(Rhodospirillum rubrum)中提取的細菌色素體(chromatophores)外膜上的F0F1-ATPases.結果 0.5 mT,4.7、12.0、60.0、72.0、84.0、96.0 Hz磁場對F0F1-ATPases水解起促進作用,0.5 mT、24.0 Hz磁場對F0F1-ATPases水解起抑製作用,差異均有統計學意義(P＜0.05);0.3 mT,4.7、24.0、60.0 Hz的磁場對F0F1-ATPases仍有明顯影響,但0.1 mT的磁場則對F0F1-ATPases無明顯影響.0.5 mT,24.0、60.0 Hz的磁場對二環己基碳二亞胺(DCCD)抑製的F0F1-ATPases仍分彆有明顯地抑製和促進酶活力的影響,差異有統計學意義(P＜0.05).結論 影響F0F1-ATPases活力的極低頻磁場具有頻率窗口,彊度閾值在0.1～0.3 mT之間;F0F1-ATPases,特彆是F1是磁場作用的一箇重要的位點.
목적 연구겁저빈정현자장대F0F1-ATPases활력적영향급기궤제.방법 강도위0.1～0.5 mT、빈솔위4.7～96.0 Hz적자장분별처리종광합세균(Rhodospirillum rubrum)중제취적세균색소체(chromatophores)외막상적F0F1-ATPases.결과 0.5 mT,4.7、12.0、60.0、72.0、84.0、96.0 Hz자장대F0F1-ATPases수해기촉진작용,0.5 mT、24.0 Hz자장대F0F1-ATPases수해기억제작용,차이균유통계학의의(P＜0.05);0.3 mT,4.7、24.0、60.0 Hz적자장대F0F1-ATPases잉유명현영향,단0.1 mT적자장칙대F0F1-ATPases무명현영향.0.5 mT,24.0、60.0 Hz적자장대이배기기탄이아알(DCCD)억제적F0F1-ATPases잉분별유명현지억제화촉진매활력적영향,차이유통계학의의(P＜0.05).결론 영향F0F1-ATPases활력적겁저빈자장구유빈솔창구,강도역치재0.1～0.3 mT지간;F0F1-ATPases,특별시F1시자장작용적일개중요적위점.
Objective To study the effects of extremely low frequency sinusoidal magnetic fields on hydrolysis of F0F1-ATPase and its mechanism. Methods The F0F1-ATPases which was localized on the outer surface of chromatophores were prepared from the cells of Rhodospirillum rubrum and were exposed to 0.1～0.5mT, 4.7～96.0Hz magnetic fields. Results The hydrolysis activity of F0F1-ATPase was stimulated by 0.5mT, 4.7, 12.0, 60.0, 72.0, 84.0 and 96.0Hz magnetic fields respectively and inhibited by 0.5mT, 24.0Hz magnetic field(P＜0.05); 0.3mT, 4.7, 24.0 and 60.0Hz magnetic fields also distinctly affected F0F1-ATPases activity respectively (P＜0.05), whereas 0.1mT exposure caused no significant changes on that activity. When the hydrolysis activity of the F0F1-ATPases was inactivated by its inhibitor DCCD, the 0.5mT, 24.0Hz magnetic field still inhibited the hydrolysis activity of the F0F1-ATPase and 0.5mT, 60.0Hz magnetic field also had stimulating effects (P＜0.05). Conclusion The effects of magnetic fields on the hydrolysis activity of the F0F1-ATPases depend on not only magnetic frequency but also magnetic intensity. The threshold of magnetic intensity is between 0.1mT and 0.3mT. F0F1-ATPases, especially F1-portion may be an end-point of magnetic fields.
