中华肝脏病杂志
中華肝髒病雜誌
중화간장병잡지
CHINESE JOURNAL OF HEPATOLOGY
2008年
7期
481-486
,共6页
田地%曾争%田国保%崔建军
田地%曾爭%田國保%崔建軍
전지%증쟁%전국보%최건군
肝炎,乙型,慢性%肝硬化%基因%载脂蛋白B mRNA编辑酶催化多肽3G
肝炎,乙型,慢性%肝硬化%基因%載脂蛋白B mRNA編輯酶催化多肽3G
간염,을형,만성%간경화%기인%재지단백B mRNA편집매최화다태3G
Hepatitis B,chronic%Liver cirrhosis%Genes%Apolipoprotein B mRNA editing enzyme catalytic polypeptide-like 3G
目的 研究中国汉族人群载脂蛋白B mRNA编辑酶催化多肽3G(APOBEC3G)基因多态性与慢性乙型肝炎和肝硬化的关系.方法 应用焦磷酸测序,检测202例HBV感染自然痊愈者(对照组)、217例慢性乙型肝炎患者和216例乙型肝炎肝硬化患者APOBEC3G基因rs8177832位点多态性,确定其基因型及等位基因分布.同时通过建立DNA池技术初步对APOBEC3G基因的另外4个标签单核苷酸多态性位点(tagSNP)rs17000736、rs17496046,rs9622924和rs2899313进行疾病相关性的初步筛选;HBV DNA检测采用实时定量PCR.结果 rs8177832在中国汉族人群中以A/A为主要存在形式,其基因型频率和等位基因频率在对照组、慢性乙型肝炎组和乙型肝炎肝硬化组中的分布差异无统计学意义.慢性乙型肝炎组中携带G等位基因的患者HBV DNA水平为6.25 log10拷贝/ml,携带A等位基因的患者为5.54 log10拷贝/ml,t=2.111,P=0.036.rs17000736和rs9622924两位点在中国汉族人群中分别只有G/G和C/C一种单一基因型存在,rs17496046和rs2899313两位点分别以G等位基因和A等位基因为主,这4个tagSNP(rs17000736、rs17496046、rs9622924和rs2899313)在对照组、慢性乙型肝炎组和乙型肝炎肝硬化组中等位基因频率差异均无统计学意义.结论 汉族人群APOBEC3G基因中rs8177832位点的多态性可能与HBV的复制有关,但这5个tagSNP可能与HBV感染后疾病的进展无关.
目的 研究中國漢族人群載脂蛋白B mRNA編輯酶催化多肽3G(APOBEC3G)基因多態性與慢性乙型肝炎和肝硬化的關繫.方法 應用焦燐痠測序,檢測202例HBV感染自然痊愈者(對照組)、217例慢性乙型肝炎患者和216例乙型肝炎肝硬化患者APOBEC3G基因rs8177832位點多態性,確定其基因型及等位基因分佈.同時通過建立DNA池技術初步對APOBEC3G基因的另外4箇標籤單覈苷痠多態性位點(tagSNP)rs17000736、rs17496046,rs9622924和rs2899313進行疾病相關性的初步篩選;HBV DNA檢測採用實時定量PCR.結果 rs8177832在中國漢族人群中以A/A為主要存在形式,其基因型頻率和等位基因頻率在對照組、慢性乙型肝炎組和乙型肝炎肝硬化組中的分佈差異無統計學意義.慢性乙型肝炎組中攜帶G等位基因的患者HBV DNA水平為6.25 log10拷貝/ml,攜帶A等位基因的患者為5.54 log10拷貝/ml,t=2.111,P=0.036.rs17000736和rs9622924兩位點在中國漢族人群中分彆隻有G/G和C/C一種單一基因型存在,rs17496046和rs2899313兩位點分彆以G等位基因和A等位基因為主,這4箇tagSNP(rs17000736、rs17496046、rs9622924和rs2899313)在對照組、慢性乙型肝炎組和乙型肝炎肝硬化組中等位基因頻率差異均無統計學意義.結論 漢族人群APOBEC3G基因中rs8177832位點的多態性可能與HBV的複製有關,但這5箇tagSNP可能與HBV感染後疾病的進展無關.
목적 연구중국한족인군재지단백B mRNA편집매최화다태3G(APOBEC3G)기인다태성여만성을형간염화간경화적관계.방법 응용초린산측서,검측202례HBV감염자연전유자(대조조)、217례만성을형간염환자화216례을형간염간경화환자APOBEC3G기인rs8177832위점다태성,학정기기인형급등위기인분포.동시통과건립DNA지기술초보대APOBEC3G기인적령외4개표첨단핵감산다태성위점(tagSNP)rs17000736、rs17496046,rs9622924화rs2899313진행질병상관성적초보사선;HBV DNA검측채용실시정량PCR.결과 rs8177832재중국한족인군중이A/A위주요존재형식,기기인형빈솔화등위기인빈솔재대조조、만성을형간염조화을형간염간경화조중적분포차이무통계학의의.만성을형간염조중휴대G등위기인적환자HBV DNA수평위6.25 log10고패/ml,휴대A등위기인적환자위5.54 log10고패/ml,t=2.111,P=0.036.rs17000736화rs9622924량위점재중국한족인군중분별지유G/G화C/C일충단일기인형존재,rs17496046화rs2899313량위점분별이G등위기인화A등위기인위주,저4개tagSNP(rs17000736、rs17496046、rs9622924화rs2899313)재대조조、만성을형간염조화을형간염간경화조중등위기인빈솔차이균무통계학의의.결론 한족인군APOBEC3G기인중rs8177832위점적다태성가능여HBV적복제유관,단저5개tagSNP가능여HBV감염후질병적진전무관.
Objective To investigate the associations between polymorphisms of apolipoprotein B mRNA editing enzyme catalytic polypeptide-like 3G (APOBEC3G) and different outcomes of HBV infection in the Chinese Han population. Methods Six hundred thirty-five chronic hepatitis B patients were divided into 3 groups: 202, 217 and 216 patients were HBV cleared, chronic hepatitis B, and with liver cirrhosis, respectively. Five tagSNPs (rs8177832, rs17000736, rs 17496046, rs9622924 and rs2899313) were genotyped by pyrosequencing. HBV viral loads were determined by real-time PCR method. Chi square was used for statistics. Results The majority of rs8177832 allele was A/A and the frequencies of rs8177832 allele among these groups were not significantly different (P > 0.05). HBV viral loads were higher in chronic hepatitis B patients with G allele than in chronic hepatitis B patients with A allele (P < 0.05). The rs17000736 and rs9622924 alleles were found only in G/G and C/C genotypes. There were also no significant differences in the other four SNPs alleles (rs17000736, rs17496046, rs9622924 and rs2899313) in these groups (P > 0.05). Conclusions rs8177832, rs17000736, rs17496046, rs17000736 and rs2899313 of the APOBEC3G gene might not be associated with HBV persistent infection in patients in this study. However, the rs8177832 polymorphism may be involved in inhibiting HBV replication.
