中华放射肿瘤学杂志
中華放射腫瘤學雜誌
중화방사종류학잡지
CHINESE JOURNAL OF RADIATION ONCOLOGY
2010年
4期
373-376
,共4页
杨方%王若雨%隋晓梅%冀学宁
楊方%王若雨%隋曉梅%冀學寧
양방%왕약우%수효매%기학저
X射线%细胞系,鼻咽肿瘤%多药耐药基因
X射線%細胞繫,鼻嚥腫瘤%多藥耐藥基因
X사선%세포계,비인종류%다약내약기인
X-ray%Cell line,nasopharyngeal neoplasms%Multidrug resistance gene
目的 RT-PCR法研究累积高剂量X射线照射后多药耐药基因MDR1 mRNA表达随时间的变化趋势,并且探讨Bcl-2、MMP7与MDR1的关系.方法 选取人鼻咽鳞癌CNE1细胞系进行体外培养及X射线照射,累积剂量50 Gy.分别收集CNE1细胞和X射线照后得到的CNE1R细胞,利用MTT法检测CNE1、CNE1R细胞对顺铂的耐药性;RT-PCR技术检测MDR1 mRNA的表达.选取CNE1和MDR1 mRNA表达最高的一组CNE1R细胞检测Bcl-2、MMP7 mRNA的表达情况.结果 CNE1R细胞对顺铂产生耐药性.RT-PCR检测CNE1细胞MDR1 mRNA半定量灰度值为0.47±0.04,照射50 Gy后1、7、21、28、35、42、49 d的CNE1R细胞MDR1 mRNA半定量灰度值均高于CNE1细胞,分别为0.67±0.06(t=-5.44,P=0.003)、0.70±0.01(t=-5.90,P=0.002)、0.73±0.01(t=-6.45,P=0.001)、0.67±0.03(t=-3.97,P=0.011)、0.65±0.01(t=-4.43,P=0.007)、0.62±0.05(t=-2.64,P=0.046)、0.62±0.02(t=-3.34,P=0.021).RT-PCR法检测CNE1细胞和CNE1R细胞中Bcl-2 mRNA表达半定量灰度值不同,分别为0.55±0.02和1.05±0.04(t=-9.93,P=0.000);MMP7 mRNA表达半定量灰度值也不同,分别为0.51±0.01和0.82±0.02(t=-8.51,P=0.000).结论 X射线累积照射50 Gy后CNE1R细胞内MDR1基因表达高于照前CNE1细胞,可能与Bcl-2、MMP7基因表达的同步变化有关.
目的 RT-PCR法研究纍積高劑量X射線照射後多藥耐藥基因MDR1 mRNA錶達隨時間的變化趨勢,併且探討Bcl-2、MMP7與MDR1的關繫.方法 選取人鼻嚥鱗癌CNE1細胞繫進行體外培養及X射線照射,纍積劑量50 Gy.分彆收集CNE1細胞和X射線照後得到的CNE1R細胞,利用MTT法檢測CNE1、CNE1R細胞對順鉑的耐藥性;RT-PCR技術檢測MDR1 mRNA的錶達.選取CNE1和MDR1 mRNA錶達最高的一組CNE1R細胞檢測Bcl-2、MMP7 mRNA的錶達情況.結果 CNE1R細胞對順鉑產生耐藥性.RT-PCR檢測CNE1細胞MDR1 mRNA半定量灰度值為0.47±0.04,照射50 Gy後1、7、21、28、35、42、49 d的CNE1R細胞MDR1 mRNA半定量灰度值均高于CNE1細胞,分彆為0.67±0.06(t=-5.44,P=0.003)、0.70±0.01(t=-5.90,P=0.002)、0.73±0.01(t=-6.45,P=0.001)、0.67±0.03(t=-3.97,P=0.011)、0.65±0.01(t=-4.43,P=0.007)、0.62±0.05(t=-2.64,P=0.046)、0.62±0.02(t=-3.34,P=0.021).RT-PCR法檢測CNE1細胞和CNE1R細胞中Bcl-2 mRNA錶達半定量灰度值不同,分彆為0.55±0.02和1.05±0.04(t=-9.93,P=0.000);MMP7 mRNA錶達半定量灰度值也不同,分彆為0.51±0.01和0.82±0.02(t=-8.51,P=0.000).結論 X射線纍積照射50 Gy後CNE1R細胞內MDR1基因錶達高于照前CNE1細胞,可能與Bcl-2、MMP7基因錶達的同步變化有關.
목적 RT-PCR법연구루적고제량X사선조사후다약내약기인MDR1 mRNA표체수시간적변화추세,병차탐토Bcl-2、MMP7여MDR1적관계.방법 선취인비인린암CNE1세포계진행체외배양급X사선조사,루적제량50 Gy.분별수집CNE1세포화X사선조후득도적CNE1R세포,이용MTT법검측CNE1、CNE1R세포대순박적내약성;RT-PCR기술검측MDR1 mRNA적표체.선취CNE1화MDR1 mRNA표체최고적일조CNE1R세포검측Bcl-2、MMP7 mRNA적표체정황.결과 CNE1R세포대순박산생내약성.RT-PCR검측CNE1세포MDR1 mRNA반정량회도치위0.47±0.04,조사50 Gy후1、7、21、28、35、42、49 d적CNE1R세포MDR1 mRNA반정량회도치균고우CNE1세포,분별위0.67±0.06(t=-5.44,P=0.003)、0.70±0.01(t=-5.90,P=0.002)、0.73±0.01(t=-6.45,P=0.001)、0.67±0.03(t=-3.97,P=0.011)、0.65±0.01(t=-4.43,P=0.007)、0.62±0.05(t=-2.64,P=0.046)、0.62±0.02(t=-3.34,P=0.021).RT-PCR법검측CNE1세포화CNE1R세포중Bcl-2 mRNA표체반정량회도치불동,분별위0.55±0.02화1.05±0.04(t=-9.93,P=0.000);MMP7 mRNA표체반정량회도치야불동,분별위0.51±0.01화0.82±0.02(t=-8.51,P=0.000).결론 X사선루적조사50 Gy후CNE1R세포내MDR1기인표체고우조전CNE1세포,가능여Bcl-2、MMP7기인표체적동보변화유관.
Objective To invstigate the effect of high dose X-ray irradiation on the expression of multidrug resistance-1 (MDR1), Bcl-2, MMP7 genes. Methods A nasopharyngeal carcinoma cell line,CNE1, were irradiated with a total dose of 50 Gy. The resistance to the cisplatin of CNE1 cells and the irradiated CNE1 (CNE1 R) cells was detected by MTT. mRNAs expression of MDR1 , Bcl-2 and MMP7 was measured by quantitative RT-PCR. Results The expression of MDR1 increased in CNE1 R cells. The semiquantitative A value of MDR1 mRNA was 0.47 ±0.04, and the value of CNE1R cells (1st, 7th, 21st,28th, 35th, 42nd and 49th days after irradiation) were 0.67 ± 0. 06 (t = -5.44, P = 0. 003) ,0.70 ± 0. 01(t=-5.90,P=0. 002),0.73±0. 01(t= -6. 45,P=0. 001) ,0. 67 ± 0. 03 (t= -3.97,P=0.011),0.65 ±0.01(t = -4.43,P=0. 007),0. 62±0. 05(t= -2. 64,P=0.046) and 0.62 ±0.02(t = -3.34,P=0.021), respectively. Bcl-2 mRNA expression were 0.55 ±0.02 and 1.05 ±0.04(t = -9.93,P=0. 000) and MMP7 mRNA expression were 0.51 ±0.01 and 0.82 ±0.02(t = -8.51,P=0.000) in CNE1and CNE1 R cells. Conclusions The MDR1 expression was increased after a total dose of50 Gy irradiation,which may be related to the synchronous change of Bcl-2 and MMP7 genes.
