CAJ | 학술논문

目的观察CD4+CD25+T细胞(Treg)对小鼠同种异体心脏移植的免疫调节作用.方法流式细胞仪检测正常小鼠和胸腺切除+PC61小鼠淋巴结、脾脏和外周血的Treg的比例.将供体鼠BALB/C心脏移植到受体鼠B6腹腔内,观察对照组(n=6)、胸腺切除组(THY,n=8)、hCTLA4Ig组(n=8)、DST+hCTLA4Ig组(n=8)和THY+PC61+DST+hCTLA4Ig组(n=6)小鼠心脏移植后生存时间和移植心脏病理学检查.结果正常B6小鼠淋巴结、脾脏和外周血的Treg的比例分别为5.1%、4.5%和1. 7%,明显高于胸腺切除+PC61处理组(1. 8%、1.7%、0.7%).移植心脏平均存活时间在对照组和胸腺切除组分别为(8.2±2.9)d和(7.6±3.0)d,两组间差异无统计学意义(P＞0.05);而在hCTLA4Ig组和DST+hCTLA4Ig组分别为(43.0±11.8)d和(135.0±29.7)d,均较对照组或胸腺切除组明显延长(P＜0.01);THY+PC61+DST+hCTLA4Ig组移植心脏平均存活时间(25.8±8.9)d,也明显较对照组明显延长,但短于hCTLA4Ig组和DST+hCTLA4Ig组(P＜0.01).DST+hCTLA4Ig组移植的心脏存活时间(135.0±29.7)d明显高于其他各组(P＜0.01),其病理组织学表现为间质内有较多的淋巴细胞浸润,伴毛细血管增生,管壁增厚,间质纤维化.结论 CD4+CD25+T细胞水平对同种异体心脏移植的免疫耐受具有免疫调节作用.
목적 관찰CD4+CD25+T세포(Treg)대소서동충이체심장이식적면역조절작용.방법 류식세포의검측정상소서화흉선절제+PC61소서림파결、비장화외주혈적Treg적비례.장공체서BALB/C심장이식도수체서B6복강내,관찰대조조(n=6)、흉선절제조(THY,n=8)、hCTLA4Ig조(n=8)、DST+hCTLA4Ig조(n=8)화THY+PC61+DST+hCTLA4Ig조(n=6)소서심장이식후생존시간화이식심장병이학검사.결과 정상B6소서림파결、비장화외주혈적Treg적비례분별위5.1%、4.5%화1. 7%,명현고우흉선절제+PC61처리조(1. 8%、1.7%、0.7%).이식심장평균존활시간재대조조화흉선절제조분별위(8.2±2.9)d화(7.6±3.0)d,량조간차이무통계학의의(P＞0.05);이재hCTLA4Ig조화DST+hCTLA4Ig조분별위(43.0±11.8)d화(135.0±29.7)d,균교대조조혹흉선절제조명현연장(P＜0.01);THY+PC61+DST+hCTLA4Ig조이식심장평균존활시간(25.8±8.9)d,야명현교대조조명현연장,단단우hCTLA4Ig조화DST+hCTLA4Ig조(P＜0.01).DST+hCTLA4Ig조이식적심장존활시간(135.0±29.7)d명현고우기타각조(P＜0.01),기병리조직학표현위간질내유교다적림파세포침윤,반모세혈관증생,관벽증후,간질섬유화.결론 CD4+CD25+T세포수평대동충이체심장이식적면역내수구유면역조절작용.
Objective To investigate the immunoregulation effects of CD4 + CD25 + T cells in mice heart allograft transplantation. Methods Flow cytometry was used to analyze the contents of CD4 + CD25 +T regulatory cells (Tregs) of the lymph nodes, spleen and blood in the normal mice group and the thymusectomy (THY) + PC61 group. BALB/C mice served as the donors and C57BL/6 (B6) mice as the recipients. Five groups were established, including control group ( n = 6 ), THY group ( n = 8 ), hCTLA4Ig group ( n = 8 ), DST ( donor-specific T-depleted spleen cells) + hCTLA4Ig group ( n = 8) and THY + PC61+ DST + hCTLA4Ig group (n = 6). The survival time after heart allograft transplantation was observed and pathological examination was done in different groups. Results In control group, the rate of Tregs in lymph nodes, spleen and blood was 5. 1%, 4. 5% and 1.7% respectively, which was significantly higher than in THY + PC61 group ( 1. 8% , 1. 7% and 0. 7% respectively). The average survival time of control and THY groups was 8. 2 ± 2.9 and 7.6 ± 3. 0 days respectively ( P ＞ 0. 05 ). The average survival time of hCTLA4Ig and DST + hCTLA4Ig groups was 43.0 ± 11.8 and 135.0 ± 29. 7 days respectively, which was significantly longer than in control group or THY group ( P ＜0. 01 ). The average survival time of THY +PC61 + DST + hCTLA4Ig group was 25.8 ± 8.9 days, which was significantly longer than in control group,but shorter than in hCTLA4Ig group or DST + hCTLA4Ig group ( P ＜ 0. 01 ). The survival time in DST +hCTLA4Ig group was 135.0 ± 29. 7 days, which was significantly longer than other groups ( P ＜ 0. 01 ).The pathological examination revealed that there were more lymphocytes infiltration and capillary vessel proliferation in the desmohemoblast in the transplanted heart of DST + hCTLA4Ig group. Conclusion CD4 +CD25 +T cells regulate the immune tolerance in the allograft transplantation.