中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2012年
1期
80-82
,共3页
徐涛%李乐平%石玉龙%苗瑞政%盛红光
徐濤%李樂平%石玉龍%苗瑞政%盛紅光
서도%리악평%석옥룡%묘서정%성홍광
膜型基质金属蛋白酶-1%血管内皮生长因子%肿瘤微血管密度%转移%预后
膜型基質金屬蛋白酶-1%血管內皮生長因子%腫瘤微血管密度%轉移%預後
막형기질금속단백매-1%혈관내피생장인자%종류미혈관밀도%전이%예후
Membrane-type matrix metalloprotenise-1%Vascular endothelial growth factor%Microvessel density%Invasion%Prognosis
目的 探讨在浸润性肝细胞肝癌(HCC)中,膜型基质金属蛋白酶-1(MT1-MMP)的表达和意义.方法 随机选取2004年1月至2006年12月间根治性手术切除的HCC标本123例,通过对肿瘤的个数、包膜是否完整、门静脉有无癌栓、有无肝外转移等标准的划分,将其分为浸润转移组(74)及无浸润组(49).术前所有病例均采用酶联免疫吸附试验(ELISA)测定血清血管内皮生长因子(VEGF)水平；并通过免疫组织化学法(ABC),测定两组术后标本癌组织的MT1-MMP蛋白及肿瘤微血管密度(MVD)的表达；定量聚合酶链反应(PCR)测定癌组织MT1-MMP mRNA的表达.结果 浸润组术前血清VEGF:(1 33.89±68.56) μg/L；无浸润组:(100.64±81.37) μg/L,差异有统计学意义(P＜0.01).MT1-MMP主要定位表达在肝癌细胞的胞膜和间质.在浸润组中,MT1-MMP蛋白及mRNA表达量明显高于无浸润组(P＜0.05)；浸润组MVD:11.24±1.49,无浸润组:8.11±2.51,两者比较浸润组MVD的形成量明显增多(P＜0.01).结论 在浸润性肝细胞肝癌中,MT1-MMP的表达明显增高,并伴随血清VEGF的表达上调、癌组织内新生肿瘤微血管的形成增多.MT1-MMP高表达可作为浸润性肝癌的判定指标,并提示临床预后较差.
目的 探討在浸潤性肝細胞肝癌(HCC)中,膜型基質金屬蛋白酶-1(MT1-MMP)的錶達和意義.方法 隨機選取2004年1月至2006年12月間根治性手術切除的HCC標本123例,通過對腫瘤的箇數、包膜是否完整、門靜脈有無癌栓、有無肝外轉移等標準的劃分,將其分為浸潤轉移組(74)及無浸潤組(49).術前所有病例均採用酶聯免疫吸附試驗(ELISA)測定血清血管內皮生長因子(VEGF)水平；併通過免疫組織化學法(ABC),測定兩組術後標本癌組織的MT1-MMP蛋白及腫瘤微血管密度(MVD)的錶達；定量聚閤酶鏈反應(PCR)測定癌組織MT1-MMP mRNA的錶達.結果 浸潤組術前血清VEGF:(1 33.89±68.56) μg/L；無浸潤組:(100.64±81.37) μg/L,差異有統計學意義(P＜0.01).MT1-MMP主要定位錶達在肝癌細胞的胞膜和間質.在浸潤組中,MT1-MMP蛋白及mRNA錶達量明顯高于無浸潤組(P＜0.05)；浸潤組MVD:11.24±1.49,無浸潤組:8.11±2.51,兩者比較浸潤組MVD的形成量明顯增多(P＜0.01).結論 在浸潤性肝細胞肝癌中,MT1-MMP的錶達明顯增高,併伴隨血清VEGF的錶達上調、癌組織內新生腫瘤微血管的形成增多.MT1-MMP高錶達可作為浸潤性肝癌的判定指標,併提示臨床預後較差.
목적 탐토재침윤성간세포간암(HCC)중,막형기질금속단백매-1(MT1-MMP)적표체화의의.방법 수궤선취2004년1월지2006년12월간근치성수술절제적HCC표본123례,통과대종류적개수、포막시부완정、문정맥유무암전、유무간외전이등표준적화분,장기분위침윤전이조(74)급무침윤조(49).술전소유병례균채용매련면역흡부시험(ELISA)측정혈청혈관내피생장인자(VEGF)수평；병통과면역조직화학법(ABC),측정량조술후표본암조직적MT1-MMP단백급종류미혈관밀도(MVD)적표체；정량취합매련반응(PCR)측정암조직MT1-MMP mRNA적표체.결과 침윤조술전혈청VEGF:(1 33.89±68.56) μg/L；무침윤조:(100.64±81.37) μg/L,차이유통계학의의(P＜0.01).MT1-MMP주요정위표체재간암세포적포막화간질.재침윤조중,MT1-MMP단백급mRNA표체량명현고우무침윤조(P＜0.05)；침윤조MVD:11.24±1.49,무침윤조:8.11±2.51,량자비교침윤조MVD적형성량명현증다(P＜0.01).결론 재침윤성간세포간암중,MT1-MMP적표체명현증고,병반수혈청VEGF적표체상조、암조직내신생종류미혈관적형성증다.MT1-MMP고표체가작위침윤성간암적판정지표,병제시림상예후교차.
Objective To investigate effect and expression of membrane type-1 matrix metalloproteinase ( MT1 -MMP,MMP-14) in invasive hepatocellular carcinoma (HCC).Methods We selected 123 patients with HCC randomly from January 2004 to December 2006 which underwent radical hepatectomy,they were divided into the invasion group (n =74) and the non invasion group (n =49) by the standards including tumor size,number,complete capsule,portal vein thrombosis and extrahepatic metastasis.The serum vascular endothelial growth factor (VEGF) was measured with the quantitative sandwich enzymelinked immunosorbent assay (ELISA) three days before operation.With three-step immunohistochemical technique ( ABC),to determine protein expression of MT1-MMP and microvessel density (MVD) in postoperative tissue samples in the two groups,and mRNA expression of MT1-MMP was measured by quantitative real-time polymerase chain reaction (PCR).Results The preoperative serum VEGF in the invasion group (133.89 ± 68.56 ) μg/L was significantly elevated as compared to that in non invasion group ( 100.64 ± 81.37 ) μg/L ( P ＜ 0.01 ).Tissue location of MT1 -MMP was mainly at membrane and in cytoplasm of HCC cells.In invasive HCC,mRNA and protein expression of MT1-MMP were significantly increased compared to that in no invasive HCC (P ＜ 0.05 ),and MVD expression increased accordingly (P＜0.01).Conclusion In invasive HCC,expression of MT1-MMP was significantly increased,and companied with increased secretion of VEGF,also the formation of tumor angiogenesis in HCC increased.High expression of MT1-MMP in postoperative tissue samples could be used as a valuable predictor for invasive HCC,and it prompted poor clinical prognosis.
