中华肿瘤杂志
中華腫瘤雜誌
중화종류잡지
CHINESE JOURNAL OF ONCOLOGY
2008年
9期
702-705
,共4页
董辉%丛文玲%朱忠政%王斌%冼志红%俞花
董輝%叢文玲%硃忠政%王斌%冼誌紅%俞花
동휘%총문령%주충정%왕빈%승지홍%유화
肝细胞癌%肝内胆管癌%病理诊断%免疫组织化学
肝細胞癌%肝內膽管癌%病理診斷%免疫組織化學
간세포암%간내담관암%병리진단%면역조직화학
Hepatocellular carcinoma%Intrahepatic cholangiocarcinoma%Pathological diagnosis%Immunohistochemistry
目的 评价一组免疫组化标志物在诊断和鉴别诊断肝细胞癌(HCC)和肝内胆管癌(ICC)中的价值.方法 对手术切除的90例HCC和80例ICC分别进行石蜡包埋肝细胞1(Hep Par 1)、多克隆性癌胚抗原(pCEA)、CD34、CD10、CD105、多药耐药相关蛋白3(MRP-3)、环氧合酶2(COX-2)、黏糖蛋白1(MUC-1)、水通道蛋白1(AQP-1)和CK19等10种抗体的免疫组化染色,比较其表达阳性率的差异性.结果 Hep Par 1、pCEA、CD34、CD10、CD105、MRP-3和COX-2在HCC的表达阳性率分别为85.6%、82.2%、87.8%、18.9%、8.9%、11.1%和48.9%,MUC-1、AQP-1和CK19在ICC的表达阳性率分别为73.8%、65%和92.5%.结论 HCC的一线诊断抗体由Hep Par 1和CD34组成,二线诊断抗体由pCEA和COX-2组成;ICC的一线诊断抗体由MUC-1和CK19组成,二线诊断抗体为AQP-1.
目的 評價一組免疫組化標誌物在診斷和鑒彆診斷肝細胞癌(HCC)和肝內膽管癌(ICC)中的價值.方法 對手術切除的90例HCC和80例ICC分彆進行石蠟包埋肝細胞1(Hep Par 1)、多剋隆性癌胚抗原(pCEA)、CD34、CD10、CD105、多藥耐藥相關蛋白3(MRP-3)、環氧閤酶2(COX-2)、黏糖蛋白1(MUC-1)、水通道蛋白1(AQP-1)和CK19等10種抗體的免疫組化染色,比較其錶達暘性率的差異性.結果 Hep Par 1、pCEA、CD34、CD10、CD105、MRP-3和COX-2在HCC的錶達暘性率分彆為85.6%、82.2%、87.8%、18.9%、8.9%、11.1%和48.9%,MUC-1、AQP-1和CK19在ICC的錶達暘性率分彆為73.8%、65%和92.5%.結論 HCC的一線診斷抗體由Hep Par 1和CD34組成,二線診斷抗體由pCEA和COX-2組成;ICC的一線診斷抗體由MUC-1和CK19組成,二線診斷抗體為AQP-1.
목적 평개일조면역조화표지물재진단화감별진단간세포암(HCC)화간내담관암(ICC)중적개치.방법 대수술절제적90례HCC화80례ICC분별진행석사포매간세포1(Hep Par 1)、다극륭성암배항원(pCEA)、CD34、CD10、CD105、다약내약상관단백3(MRP-3)、배양합매2(COX-2)、점당단백1(MUC-1)、수통도단백1(AQP-1)화CK19등10충항체적면역조화염색,비교기표체양성솔적차이성.결과 Hep Par 1、pCEA、CD34、CD10、CD105、MRP-3화COX-2재HCC적표체양성솔분별위85.6%、82.2%、87.8%、18.9%、8.9%、11.1%화48.9%,MUC-1、AQP-1화CK19재ICC적표체양성솔분별위73.8%、65%화92.5%.결론 HCC적일선진단항체유Hep Par 1화CD34조성,이선진단항체유pCEA화COX-2조성;ICC적일선진단항체유MUC-1화CK19조성,이선진단항체위AQP-1.
Objective To evaluate the significance of a panel of immunohistochemieal markers for distinguishing hepatocellular carcinoma (HCC) from intrahepatic cholangiocarcinoma (ICC). Methods Ten markers including bepatecyte paraffin 1 (Hep Par 1 ), polyclonal carcinoembryonic antigen (pCEA), CD34, CD10, CDI05, muhidrug resistance-associated protein-3 (MRP-3), cyclcoxygenase-2 (COX-2), mucinous glycoprotein-1 (MUC-1), aquaporin-1 (AQP-1) and CK19 were immunohistecbemically stained in the samples from 90 surgically resected HCC and 80 ICC, respectively, and the positive rate of their expression were compared statistically. Results The positive expression rates of Hep Par 1, pCEA, CD34, CD10, CD105, MRP-3, COX-2 were 85.6%, 82.2%, 87.8%, 18.9%, 8.9%, 11.1% and 48.9%, respectively, in HCC. While the positive expression rates of MUC-1, AQP-1 and CK19 were 73.8%, 65% and 92.5%, respectively, in ICC. Conclusion Based on our results, Hep Par 1 and CD34 can be used as the first line markers, and pCEA and COX-2 as the second line makers, for differential diagnosis of hepatecellular carcinoma from intrahepatie cholangiocarcinoma. While MUC-1 and CK19 can be used as the first line markers and AQP-1 as the second one for the differential diagnosis of intrabepatic cholangiocarcinoma from bepatocellular carcinoma.
