生物技术通报
生物技術通報
생물기술통보
BIOTECHNOLOGY BULLETIN
2009年
12期
167-171
,共5页
白腐菌%固体发酵%木质素降解率%诱导剂
白腐菌%固體髮酵%木質素降解率%誘導劑
백부균%고체발효%목질소강해솔%유도제
White-rot fungus%Solid-state fermentation%Lignin degradation rate%Inducer
通过正交试验对3种白腐菌混合菌降解竹材木质素的条件进行优化,结果表明,在温度为32℃、pH3.0、固体发酵时间20 d、培养液与竹材基质质量百分比110%时降解木质素的效率最高.在此基础上,研究了两种诱导剂对白腐菌混合菌降解木质素的影响.结果表明,两种诱导剂均能促进木质素的降解,其中H_2O_2在浓度1%时,木质素降解率高达62.9%,苯甲酸在浓度0.1%时,木质素降解率最高,为67.8%.
通過正交試驗對3種白腐菌混閤菌降解竹材木質素的條件進行優化,結果錶明,在溫度為32℃、pH3.0、固體髮酵時間20 d、培養液與竹材基質質量百分比110%時降解木質素的效率最高.在此基礎上,研究瞭兩種誘導劑對白腐菌混閤菌降解木質素的影響.結果錶明,兩種誘導劑均能促進木質素的降解,其中H_2O_2在濃度1%時,木質素降解率高達62.9%,苯甲痠在濃度0.1%時,木質素降解率最高,為67.8%.
통과정교시험대3충백부균혼합균강해죽재목질소적조건진행우화,결과표명,재온도위32℃、pH3.0、고체발효시간20 d、배양액여죽재기질질량백분비110%시강해목질소적효솔최고.재차기출상,연구료량충유도제대백부균혼합균강해목질소적영향.결과표명,량충유도제균능촉진목질소적강해,기중H_2O_2재농도1%시,목질소강해솔고체62.9%,분갑산재농도0.1%시,목질소강해솔최고,위67.8%.
In this paper, the conditions for White-rot fungus mixed bacteria degrading lignin were optimized through the orthogonalexperiments.The results showed that when the temperature was 32℃, pH30, the solid-state fermentation time was 20 d, the culture medium and the substrate quality bamboo was 110%, and the efficiency of lignin degradation was up to a maximum.On this basis, the impact of two kinds of inducing agents acting on mixed white rot fungi degrade lignin was studied.The results showed that two kinds of inducing agents can promote the degradation of lignin, in which the concentration of H_2O_2 at 1%, lignin degradation rate as high as 62.9%, benzoic acid in the concentration of 0.1%, and the highest rate of lignin degradation, to 67.8%.