CAJ | 학술논문

应用聚丙烯酰胺凝胶电泳结合生化染色方法分别对雌雄泰山赤鳞鱼10种组织中苹果酸酶同工酶酶谱进行分析.结果表明,在所取的10种组织中,雌性泰山赤鳞鱼在心脏、性腺、肾、眼、鳃和脾6种组织中存在苹果酸酶,而雄性泰山赤鳞鱼只在心、肌肉、性腺、眼和鳃5种组织中存在苹果酸酶.雌性鱼心脏组织中只有1条ME-2带,而雄性鱼心脏组织含有ME-1和ME-2 2条酶带;卵巢中含有ME-1 和ME-2 2条酶带,而精巢中只含有ME-1 1条带;雌性鱼的眼组织含有ME-1带,而雄性鱼眼中含有ME-2带.肾和脾组织只在雌性鱼中含有苹果酸酶酶带,而肌肉组织只在雄性鱼中含有苹果酸酶.苹果酸酶的表型在泰山赤鳞鱼不同性别同一组织和同一性别不同组织之间都存在明显差异,但在同一性别的不同个体之间不存在差异.该研究结果为赤鳞鱼种群的发育遗传学、品种改良和定向育种提供了基础资料,为赤鳞鱼的开发和保护奠定基础.
응용취병희선알응효전영결합생화염색방법분별대자웅태산적린어10충조직중평과산매동공매매보진행분석.결과표명,재소취적10충조직중,자성태산적린어재심장、성선、신、안、새화비6충조직중존재평과산매,이웅성태산적린어지재심、기육、성선、안화새5충조직중존재평과산매.자성어심장조직중지유1조ME-2대,이웅성어심장조직함유ME-1화ME-2 2조매대;란소중함유ME-1 화ME-2 2조매대,이정소중지함유ME-1 1조대;자성어적안조직함유ME-1대,이웅성어안중함유ME-2대.신화비조직지재자성어중함유평과산매매대,이기육조직지재웅성어중함유평과산매.평과산매적표형재태산적린어불동성별동일조직화동일성별불동조직지간도존재명현차이,단재동일성별적불동개체지간불존재차이.해연구결과위적린어충군적발육유전학、품충개량화정향육충제공료기출자료,위적린어적개발화보호전정기출.
By using polyacrylamide gel electrophoresis and biochemical straining method, malic enzyme isozyme in 10 organs of male or female Varicorhinus macrolepis were analyzed. The results showed that malic enzyme exists in heart, gonads, eyes and gills tissues in both female and male V. macrolepis. There was only one ME-2 in heart and ME-1 in eye of female fish, while there were ME-1 and ME-2 in heart and ME-2 in eye of male fish. Ovary contained ME-1 and ME-2, while spermary only contained ME-1. In kidney and spleen tissues, malic enzyme was found only in female fish, while in muscle, malic enzyme existed only in male fish. The phenotypic of malic enzyme had obvious differences no matter in the same organ, different gender of V. macrolepis or same gender, different organs, but there were no differences among different individuals of the same sex. The results will provide basic data for developmental genetics, breed improvement and directed breeding of V. macrolepis, and lay a foundation for its development and protection.