CAJ | 학술논문

目的应用双向电泳和质谱技术研究人睾丸胚胎性癌蛋白质组.方法利用固相PH梯度双向凝胶电泳技术进行分离,凝胶经银染显色后,用PDQuest 7.30软件分析2-DE图谱.选择在睾丸胚胎性癌图谱中高表达的3个蛋白点酶解,应用基质辅助激光解析电离飞行时间/飞行时间(MALDI-TOF/TOF)仪器进行串联质谱(MS/MS)鉴定.结果双向电泳成功有效筛选出差异蛋白点,并成功鉴定3种蛋白质.结论双向电泳和质谱技术可有效研究睾丸胚胎性癌总蛋白质,为从蛋白质组水平研究睾丸胚胎性癌蛋白质的表达改变提供了新的方法和途径.
목적 응용쌍향전영화질보기술연구인고환배태성암단백질조.방법 이용고상PH제도쌍향응효전영기술진행분리,응효경은염현색후,용PDQuest 7.30연건분석2-DE도보.선택재고환배태성암도보중고표체적3개단백점매해,응용기질보조격광해석전리비행시간/비행시간(MALDI-TOF/TOF)의기진행천련질보(MS/MS)감정.결과 쌍향전영성공유효사선출차이단백점,병성공감정3충단백질.결론 쌍향전영화질보기술가유효연구고환배태성암총단백질,위종단백질조수평연구고환배태성암단백질적표체개변제공료신적방법화도경.
Objective To separate and identify human testicular embryonal carcinoma proteomics using two-dimensional electrophoresis (2-DE) and mass spectrometry. Methods Immobilized pH gradient two-dimensional polyacrylamide gel electrophoresis was used to separate the total proteins of the samples. After silver staining, PDQuest 7.30 image analysis software was applied to analyze the 2-DE images. Three of the proteins highly expressed in human testicular embryonal carcinoma were identified by matrix-assisted laser adsorption/ionization-time of flight-tandem mass spectrometry (MALDI-TOF-MS/MS). Results 2-DE effectively screened the differentially expressed proteins in the carcinoma tissues.Three proteins highly expressed in the carcinoma were successfully identified. Conclusion The proteins of human testicular embryonal carcinoma can be effectively separated and analyzed using 2-DE and mass spectrometry. Proteomic analysis offers a new means for further study of this carcinoma.