CAJ | 학술논문

在传统的Tris-柠檬酸-葡萄糖(TCG)稀释液基础上,分别添加0.1 mol/L乳糖、蔗糖、海藻糖,并采用细管法对精液进行冷冻,研究不同双糖对冻融后猪精液质量的影响.结果表明:0.1 mol/L的海藻糖和蔗糖相对于对照组和0.1 mol/L的乳糖TCG稀释液能够显著改善冻融后猪精子质量,其冻融后精子的活率、顶体完整率、低渗膨胀率、平均路径速度、运动线速度、平均侧摆幅度和平均鞭打频率均明显提高(P<0.05),分别达到42.14%和40.56%,51.48%和49.84%,46.20%和43.01%,32.8 μm/s 和33.2 μm/s,60.80%和59.00%,1.70 μm 和1.62 μm,7.68 Hz 和7.62 Hz.TCG稀释液中添加0.1 mol/L海藻糖和蔗糖的猪冷冻精液体外受精的受精能力明显高于0.1 mol/L乳糖组和对照组,其卵裂率分别为41%和38%.
재전통적Tris-저몽산-포도당(TCG)희석액기출상,분별첨가0.1 mol/L유당、자당、해조당,병채용세관법대정액진행냉동,연구불동쌍당대동융후저정액질량적영향.결과표명:0.1 mol/L적해조당화자당상대우대조조화0.1 mol/L적유당TCG희석액능구현저개선동융후저정자질량,기동융후정자적활솔、정체완정솔、저삼팽창솔、평균로경속도、운동선속도、평균측파폭도화평균편타빈솔균명현제고(P<0.05),분별체도42.14%화40.56%,51.48%화49.84%,46.20%화43.01%,32.8 μm/s 화33.2 μm/s,60.80%화59.00%,1.70 μm 화1.62 μm,7.68 Hz 화7.62 Hz.TCG희석액중첨가0.1 mol/L해조당화자당적저냉동정액체외수정적수정능력명현고우0.1 mol/L유당조화대조조,기란렬솔분별위41%화38%.
In order to improve boar sperm quality parameters in frozen-thawed process,0.1 mol/L lactose,sucrose and trehalose were respectively added into the basic extenders Tris-Citric acid-Glucose(TCG).The effects of disaccharide on quality of cryopreservation of boar sperm were investigated.We found out that TCG extender with 0.1 mol/L trehalose and sucrose significantly enhanced motility,acrosome integrity rate,hypotonic expansion rate, VAP, LIN, ALH and BCF of boar sperm after freezing and thawing(P<0.05),they were 42.14% and 40.56%,51.48% and 49.84%,46.20% and 43.01%,32.8 μm/s and 33.2 μm/s,60.80% and 59.00%,1.70 μm and 1.62 μm,7.68 Hz and 7.62 Hz.The IVF rate of frozen-thawed sperm in 0.1 mol/L trehalose and sucrose is significantly higher than that of others(P<0.05). They were 41% and 38%.