CAJ | 학술논문

利用Gienlsa C-带和染色体荧光原位杂交(45S rDNA FISH)的方法对'Royal Lace'×'Highg class'的杂种后代分别进行了鉴定.结果表明:'Royal Lace'的染色体数2n=3x=36,'High class'的染色体数2n=2x=24.杂种后代的染色体数出现2n=3x=36和2n=4x=48两种类型.经Giemsa c-带和45s rDNA FISH方法对两个亲本和具2n=4x=48的杂种后代分析结果表明,杂种后代的根尖染色体C-带可观察到来自双亲的可以特异追踪的染色体带纹.FISH分析表明,杂种后代中分别有两条来自'Royal Lace'和'High Class'的染色体.两个亲本的荧光原位杂交点数分别为10和9.杂种后代染色体为2n=36的个体有14个杂交信号,可以确定两条来自'Royal Lace',另外3条来自'High Class'.杂种后代中2n=48的个体的杂交信号点为19,从而证实所获得的杂种后代的真实性.同时,对于2n=48,而杂交信号为19的多倍体起源于未减数分裂的2n雄配子体的可能性进行了探讨.综合研究结果表明,Giemsa C-带和45S rDNA FISH方法可以准确地对百合的杂种真实性进行鉴定.
이용Gienlsa C-대화염색체형광원위잡교(45S rDNA FISH)적방법대'Royal Lace'×'Highg class'적잡충후대분별진행료감정.결과표명:'Royal Lace'적염색체수2n=3x=36,'High class'적염색체수2n=2x=24.잡충후대적염색체수출현2n=3x=36화2n=4x=48량충류형.경Giemsa c-대화45s rDNA FISH방법대량개친본화구2n=4x=48적잡충후대분석결과표명,잡충후대적근첨염색체C-대가관찰도래자쌍친적가이특이추종적염색체대문.FISH분석표명,잡충후대중분별유량조래자'Royal Lace'화'High Class'적염색체.량개친본적형광원위잡교점수분별위10화9.잡충후대염색체위2n=36적개체유14개잡교신호,가이학정량조래자'Royal Lace',령외3조래자'High Class'.잡충후대중2n=48적개체적잡교신호점위19,종이증실소획득적잡충후대적진실성.동시,대우2n=48,이잡교신호위19적다배체기원우미감수분렬적2n웅배자체적가능성진행료탐토.종합연구결과표명,Giemsa C-대화45S rDNA FISH방법가이준학지대백합적잡충진실성진행감정.
In this study, hybrid plants from the crosses between ' Royal Lace' ×' High Class' were analyzed with Giemsa C - banding and 45S rDNA FISH. The results showed that, the number of chromosomes was 2n = 3x = 36 for ' Royal Lace' , In =2x = 24 for ' High Class' and In = 3x =36 or 2n =4x =48 for two different hybrids, respectively. The special C - banging patterns of two parents can be found in their hybrids. By FISH analysis, 14 hybridization Iocies were detected in the hybrids of 2n = 36. Two chromosomes from ' Royal Lace' and three chromosomes from ' High Class' can be identified clearly. And there were 19 hybridization locies to be distinguished for 2n =48 hybrids,which also suggested that these hybrids might relate to be the contributed by unreduced male gametes (2n). All of these evidences clearly confirmed that, the plants were true hybrids.