CAJ | 학술논문

采用沸水回流法从灵芝子实体中提取多糖,经Sevage法除蛋白,乙醇沉淀,离心、透析、膜分离,浓缩、冻干后得灵芝多糖.经HIO_4氧化、Smith降解及甲基化反应,并利用多糖及刚果红混合液在碱性溶液中的波长的红移变化,通过UV-VIS, IR, GC, GC-MS, NMR对灵芝水提多糖的结构特征及三螺旋体结构进行分析研究. 结果表明:灵芝多糖含有三螺旋体构型,GC-MS分析灵芝多糖的主要单糖组分为葡萄糖, 还有少量的半乳糖、甘露糖、木糖和艾杜糖,IR及~1H NMR分析多糖为β-构型, HIO_4氧化、Smith降解和甲基化分析表明:多糖主要为(1→3)糖苷键连接构型, 并伴有少量的1→6位支链键连接的结构,灵芝多糖是由D-葡萄糖单元通过β-(1→3)糖苷键连接葡聚多糖,其主要构型特征为 (1→3)β-D-线性连接的骨架结构.
채용비수회류법종령지자실체중제취다당,경Sevage법제단백,을순침정,리심、투석、막분리,농축、동간후득령지다당.경HIO_4양화、Smith강해급갑기화반응,병이용다당급강과홍혼합액재감성용액중적파장적홍이변화,통과UV-VIS, IR, GC, GC-MS, NMR대령지수제다당적결구특정급삼라선체결구진행분석연구. 결과표명:령지다당함유삼라선체구형,GC-MS분석령지다당적주요단당조분위포도당, 환유소량적반유당、감로당、목당화애두당,IR급~1H NMR분석다당위β-구형, HIO_4양화、Smith강해화갑기화분석표명:다당주요위(1→3)당감건련접구형, 병반유소량적1→6위지련건련접적결구,령지다당시유D-포도당단원통과β-(1→3)당감건련접포취다당,기주요구형특정위 (1→3)β-D-선성련접적골가결구.
Polysaccharide was extracted by boiling water reflux method from the fruiting body of Ganoderma lucidum. Additionally, the purified polysaccharide was obtained by removing protein with Sevage way, ethanol precipitation, centrifugation, run water dialysis, membrane separation, concentration and frozen-drying. The structural characteristics, chain conformation and triple-helix conformation of ganoderma lucidum polysaccharide (GLP) were distinguished by Smith degradation, methylation analysis, and the wavelength change of the red shift of the mixture of polysaccharide and Congo red in alkaline solution, as well as IR, GC-MS, NMR, and visible spectrometry. The results indicated that GLP was a linear (1→3) β-D-Glcp main chain linkage. Its monosaccharide component was predominantly composed of D-Glc, and small amount of galactose, mannose, xylose and idose, residues of branches terminated with substituted at 1→6 by a small number of single-unit β-D-Glcp side-chains, it′s also observed that the (1→3)-linked β-D- glucan contained a triple-helical conformation, which was composed of a repeating unit with a structure as below:→3)-β-D-Glcp-(1→3)-[β-D-Glcp-(1→3)-]_n-β-D-Glcp-(1→.↑6/1β-D-Glcp