CAJ | 학술논문

目的观察依维莫司等免疫抑制剂对大鼠胰岛瘤细胞(INS-1)和SD大鼠正常胰岛细胞代谢活性和功能的影响,旨在探讨依维莫司在胰岛移植免疫抑制治疗中的安全性和可行性.方法不同浓度梯度免疫抑制剂依维莫司、西罗莫司、环胞霉素A和霉酚酸酯处理INS-1和正常胰岛细胞,用MTT法检测细胞的代谢活性;用葡萄糖刺激胰岛素分泌实验检测胰岛细胞的胰岛素分泌功能.结果临床血药浓度以上的依维莫司和西罗莫司高浓度组胰岛细胞增殖抑制率低于环胞霉素A和霉酚酸酯(P＜0.05);依维莫司在临床血药浓度范围和其它免疫抑制剂一样可以抑制胰岛细胞的胰岛素分泌功能,各组间的刺激指数差异无统计学意义.结论依维莫司和西罗莫司对INS-1细胞及SD大鼠胰岛的毒性作用较小,环胞霉素A和霉酚酸酯对INS-1和SD大鼠胰岛具有较为明显的毒性作用,依维莫司有望作为新型免疫抑制剂应用于临床胰岛移植.
목적 관찰의유막사등면역억제제대대서이도류세포(INS-1)화SD대서정상이도세포대사활성화공능적영향,지재탐토의유막사재이도이식면역억제치료중적안전성화가행성.방법 불동농도제도면역억제제의유막사、서라막사、배포매소A화매분산지처리INS-1화정상이도세포,용MTT법검측세포적대사활성;용포도당자격이도소분비실험검측이도세포적이도소분비공능.결과 림상혈약농도이상적의유막사화서라막사고농도조이도세포증식억제솔저우배포매소A화매분산지(P＜0.05);의유막사재림상혈약농도범위화기타면역억제제일양가이억제이도세포적이도소분비공능,각조간적자격지수차이무통계학의의.결론 의유막사화서라막사대INS-1세포급SD대서이도적독성작용교소,배포매소A화매분산지대INS-1화SD대서이도구유교위명현적독성작용,의유막사유망작위신형면역억제제응용우림상이도이식.
Objective To investigate the effect of immunosuppression agent Everolimus on the viability and function of insuloma cells(INS-1)and pancreatic islet cells cultured in vitro.Methods INS-1 cells and islets were treated with a series of concentrations of immunosuppression agents(Everolimus,Cyclosporin A,Sirolimus and Mycophenolate Mofetil).The viability of INS-1 cells and rat pancreatic islets were determined with MTT and the function of INS-1 cells and rat pancreatic islets was evaluated with Glucose-stimulated insulin secretion assay.Results Above the clinical blood concentration,the inhibition rate of islet cell proliferation in the high concentration group of Everolimus and Sirolimus was significantly lower than that of Cyclosporin A and Mycophenolate Mofetil group(P＜0.05);Everolimus in the blood drug level,like other immunosuppressive agents,can inhibit the function of insulin secretion,and the stimulation index of each group was no significant difference.Conclusion Compared to Mycophenolate Mofetil and Cyclosporin A,Everilimus and Sirolimus demonstrate lower toxicity effect on INS-1 cells and rat pancreatic islets in vitro and Everolimus is expected as a new type of immunosuppressive agent used in clinical islet transplantation.