CAJ | 학술논문

目的观察普罗布考对动脉粥样硬化兔巨噬细胞、肝细胞HDL功能的影响,并进一步探讨普罗布考抗动脉粥样硬化的机制.方法 18只新西兰大白兔随机分为:(1)正常对照组(n=6):给予普通饮食喂养;(2)动脉粥样硬化组(n:6):饲以高脂饲料;(3)普罗布考组(n=6):在饲以高脂饮食基础上给予普罗布考[400 mg/(kg·d)].12周后取血以酶法测定血脂;利用流式细胞仪检测腹腔巨噬细胞、肝细胞细胞表面ABCA1、SR-B Ⅰ表达量;液闪计数仪检测其[~3H]胆固醇转出率.结果实验12周后,普罗布考组血清总胆固醇(TC)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C)、非高密度脂蛋白(NHDL-C)水平明显下降.普罗布考上调动脉粥样硬化兔腹腔巨噬细胞及肝细胞ABCA1及SR-BⅠ蛋白质表达水平.普罗布考组腹腔巨噬细胞、肝细胞[~3H]胆固醇转出率较动脉粥样硬化组明显上升.结论虽然普罗布考降低HDL-C,但是其通过上调腹腔巨噬细胞、肝细胞SR-BⅠ及ABCAI的表达及增加其[~3H]胆固醇转出率,改善HDL功能,具有促进动脉粥样硬化兔胆固醇逆转运(RCT)作用.
목적 관찰보라포고대동맥죽양경화토거서세포、간세포HDL공능적영향,병진일보탐토보라포고항동맥죽양경화적궤제.방법 18지신서란대백토수궤분위:(1)정상대조조(n=6):급여보통음식위양;(2)동맥죽양경화조(n:6):사이고지사료;(3)보라포고조(n=6):재사이고지음식기출상급여보라포고[400 mg/(kg·d)].12주후취혈이매법측정혈지;이용류식세포의검측복강거서세포、간세포세포표면ABCA1、SR-B Ⅰ표체량;액섬계수의검측기[~3H]담고순전출솔.결과 실험12주후,보라포고조혈청총담고순(TC)、저밀도지단백담고순(LDL-C)、고밀도지단백담고순(HDL-C)、비고밀도지단백(NHDL-C)수평명현하강.보라포고상조동맥죽양경화토복강거서세포급간세포ABCA1급SR-BⅠ단백질표체수평.보라포고조복강거서세포、간세포[~3H]담고순전출솔교동맥죽양경화조명현상승.결론 수연보라포고강저HDL-C,단시기통과상조복강거서세포、간세포SR-BⅠ급ABCAI적표체급증가기[~3H]담고순전출솔,개선HDL공능,구유촉진동맥죽양경화토담고순역전운(RCT)작용.
Objective To analyze the effect of probucol on HDL function and the its anti-atherosclerosis mecha-nism in atherosclerotic rabbits.Methods Eighteen rabbits were randomly assigned into three groups:(1)control group (n=6):normal diet for 12weeks;(2)atherosclerotic group:cholesterol diet of 12 weeks;(3)probucol group:choles-terol diet plus probucol(400mg/(kg·d))for 12 weeks.Flow cytometry and liquid scintillation spectrometry were used to measure the expressions of ABCA1 and SR-B,and cholesterol efflux rates,respectively,in peritoneal maerophage and hepatocvtes.Results Compared with atherosclerotic group,the levels of serum total cholesterol(TC),low-density lipoprotein cholesterol(LDL-C),high density lipoprotein cholesterol(HDL-C)and non-high-density lipoprotein cho-lesterol(NHDL-C)in probucol group were significantly reduced.In peritoneal macrophages and hepatocytes,the ABCA1 and SR-BI expression and[~3H]cholesterol efflux rates significantly increased in probucol group compared with that in ath-eroselerotic group.Conclusion Via up-regulating SR-Bl and ABCA1 expression and increasing cholesterol efflux rates in peritoneal macrophages and hepatocytes,probucol improves HDL function and reverse cholesterol transport(RCT).