CAJ | 학술논문

目的探讨反向模式钠钙离子交换体抑制剂KB-R7943是否对造影剂肾损伤具有保护作用.方法培养大鼠肾小管上皮细胞分别与不同浓度KB-R7943(10-5,10-6 mol/L)作用12 h后,加入造影剂作用1 h.采用LDH检测细胞损伤,倒置显微镜观察细胞形态变化,流式细胞仪检测细胞凋亡,共聚焦显微镜测定细胞内钙和反应氧产物水平,RT-PCR检测钠钙离子交换体mRNA表达.相同渗透压甘露醇作对照.数据以均数±标准差(x±s)表示,统计采用方差分析和q检验,简单直线相关分析两者相关性,以P<0.05为差异具有统计学意义.结果造影剂作用1 h诱导了明显细胞损伤和细胞凋亡,细胞内钙和反应氧产物增加,KB-R7943降低了细胞内钙和反应氧产物水平,同时降低了细胞损伤和细胞凋亡并呈剂量效应;钠钙离子交换体mRNA表达无变化.结论 KB-R794对造影剂诱导的肾小管上皮细胞损伤具有保护作用.
목적 탐토반향모식납개리자교환체억제제KB-R7943시부대조영제신손상구유보호작용.방법 배양대서신소관상피세포분별여불동농도KB-R7943(10-5,10-6 mol/L)작용12 h후,가입조영제작용1 h.채용LDH검측세포손상,도치현미경관찰세포형태변화,류식세포의검측세포조망,공취초현미경측정세포내개화반응양산물수평,RT-PCR검측납개리자교환체mRNA표체.상동삼투압감로순작대조.수거이균수±표준차(x±s)표시,통계채용방차분석화q검험,간단직선상관분석량자상관성,이P<0.05위차이구유통계학의의.결과 조영제작용1 h유도료명현세포손상화세포조망,세포내개화반응양산물증가,KB-R7943강저료세포내개화반응양산물수평,동시강저료세포손상화세포조망병정제량효응;납개리자교환체mRNA표체무변화.결론 KB-R794대조영제유도적신소관상피세포손상구유보호작용.
Objective To investigate the renoprotective effects of KB-R7943 on renal injury induced bycontrast media. Method Tubular epithelial cells were trested with varions cencentration of KB-B7943 (10-5,10-6 mol/L) for 12 hours before contrast media was used. After cells we, re incubated with contrast media (CM)(110 mgI/L) for 1 hour, cells injury was assessed by using LDH, and cell morphologic changes and cells apopto-sis were evaluated with inverted microscope and flow cytometry, respoelively. Intracellular Ca2+ and reactive oxy-gen species (ROS) were analyzed by using confocal microscope. The expression of Na+/Ca2+exchanger mRNAwas evaluated by RT-PCR. Mannitol with same osmolarity (20% mannitol, 770 mOsm/L) of CM was used as con-trol. One-way analysis of variance and q-test were used for comparison between groups. The simple linear correla-tion was employed to analyze the correlation. P < 0.05 was considered significant. Results Contrast media sig-nificantly induced tubular cells damage significantly and apoptosis at 1 hour after incubation, meanwhile, intracel-lular Ca2+ and ROS were inoreased progressively in CM group. KB-R7943 significantly attenuated cells damage andapoptosis in dose-dependent in association with decreased intracellular Ca2+ and ROS. Expression of Na+/Ca2+exchanger mRNA was not changed. Conclusions KB-R7943 has renoprotective effects on the contrast-media-in-duced renal tubular cyotoxicity