CAJ | 학술논문

目的观察环氧合酶-2(COX-2)抑制剂对肺癌细胞的放射增敏作用.方法 COX-2抑制剂LM-1685作用于体外培养的肺癌细胞A549,噻唑蓝(MTT)法检测其对肺癌细胞的抑制效果,克隆形成法检测放射敏感性,流式细胞术检测细胞周期变化.结果 COX-2抑制剂LM-1685具有明显的抑制A549细胞增殖的作用,其效应呈时间和剂量依赖性,50μmol/L LM-1685在有或没有IL-1β作用下对A549细胞均有放射增敏作用,其放射增敏比分别为1.12、1.06.50μmol/L LM-1685能去除X射线导致的A549细胞G2/M期阻滞.结论 COX-2抑制剂对肺癌细胞A549具有放射增敏作用,放射增敏机制可能与去除G2/M期阻滞有关.
목적 관찰배양합매-2(COX-2)억제제대폐암세포적방사증민작용.방법 COX-2억제제LM-1685작용우체외배양적폐암세포A549,새서람(MTT)법검측기대폐암세포적억제효과,극륭형성법검측방사민감성,류식세포술검측세포주기변화.결과 COX-2억제제LM-1685구유명현적억제A549세포증식적작용,기효응정시간화제량의뢰성,50μmol/L LM-1685재유혹몰유IL-1β작용하대A549세포균유방사증민작용,기방사증민비분별위1.12、1.06.50μmol/L LM-1685능거제X사선도치적A549세포G2/M기조체.결론 COX-2억제제대폐암세포A549구유방사증민작용,방사증민궤제가능여거제G2/M기조체유관.
Objective To investigate the radiosensitizing effects of cyclooxygenase-2 selective inhibitor LM-1685 on A549 cells in vitro.Methods A549 human lung adenocarcinoma cell line was used in this study.Cell growth kinetics Was determined using MTT assay.Cell survival was analyzed by clonogenic assay.The change of cell cycle Was measured by flow cytometry.Results LM-1685 inhibited the growth of A549 cells,showing a dose-dependent and time-dependent manner.LM-1685(50/μmol/L),either with or without IL-1β,showed the radiosensitizing effects on A549 cells,and the sensitizing enhancement ratio(SER)was 1.12 and 1.06,respectively.LM-1685(50 μmol/L)abrogated radiation-induced G2/M arrest of the tested A549 cells.Conclusions Cyclooxygenase-2 selective inhibitor can enhance the radiosensitivity of A549 cell line.Abrogation of radiation-induced G2/M arrest could be part of the mechanism.