CAJ | 학술논문

目的观察南蛇藤总萜对Hepal-6荷瘤小鼠外周血VEGF、CECs的影响.方法采用常规无菌接种技术制作小鼠肝癌Hepal-6移植性肿瘤模型,观察南蛇藤总萜高、中、低剂量(40 mg/kg、20 mg/kg、10 mg/kg)对Hepal-6荷瘤小鼠外周血VEGF、CECs表达的影响.结果南蛇藤总萜在20 mg/kg时,外周血VEGF (45.24±21.36) pg/ml、CECs (0.83±0.10)％表达明显降低,与阴性对照组(0.9％生理盐水)比较,差异有统计学意义(P＜0.01).结论南蛇藤总萜可明显降低VEGF、CECs表达,这一作用可能与南蛇藤总萜抑制肝癌血管生成有关.
목적 관찰남사등총첩대Hepal-6하류소서외주혈VEGF、CECs적영향.방법 채용상규무균접충기술제작소서간암Hepal-6이식성종류모형,관찰남사등총첩고、중、저제량(40 mg/kg、20 mg/kg、10 mg/kg)대Hepal-6하류소서외주혈VEGF、CECs표체적영향.결과 남사등총첩재20 mg/kg시,외주혈VEGF (45.24±21.36) pg/ml、CECs (0.83±0.10)％표체명현강저,여음성대조조(0.9％생리염수)비교,차이유통계학의의(P＜0.01).결론 남사등총첩가명현강저VEGF、CECs표체,저일작용가능여남사등총첩억제간암혈관생성유관.
Objective To study the effects of Celastrus orbiculatus extracts (COE) on the expression of VEGF and CECs of Hepal-6 tumor bearing mice.Methods Animal Models of Hepal-6 portability liver tumor bearing mice were established and randomly assigned to six groups (eight mice per group) as follows:untreated control group,solvent vehicle control (DMSO) group,physiological saline control group,cisplatin-treated group,and different dosages COE-treated groups (40 mg/kg,20 mg/kg,10 mg/kg).To approach the effects of COE on the expression of VEGF and CECs in Hepal-6 toumor bearing mice.Results The ELISA and flow cytometer showed that COE could significantly inhibit the expression of VEGF(45.24±21.36) pg/ml and CECs (0.83 ± 0.10) ％ compared with the physiological saline control group (P＜ 0.01 ) with the dose of 20mg/kg.Conclusion COE could significantly down-regulate the expression of VEGF and CECs.It may be related with the effect of COE in inhibiting angiogenesis of tumor.