CAJ | 학술논문

目的探讨caveolin-1在乳腺癌中的表达及其意义.方法采用免疫组化二步法,检测105例乳腺癌和50例非癌乳腺组织中caveolin-1的表达情况,同时检测组织中CK5/6、EGFR、E-cadherin表达情况,研究其与eaveolin-1表达的关系,并对105例乳腺癌患者的临床病理资料进行回顾性分析.结果 105例乳腺癌按基因分型分为基底细胞样乳腺癌20例,腔上皮A型乳腺癌22例,腔上皮B型乳腺癌23例,HER2高表达型乳腺癌23例,正常乳腺表型乳腺癌17例.Caveolin-1蛋白在乳腺癌和非癌乳腺组织的表达率分别为24.8％ (26/105),88％ (44/50).Caveolin-1在基底细胞样型,腔上皮A型,腔上皮B型,HER2高表达型,正常乳腺表型乳腺癌的表达率分别为75.0％ (15/20)、4.8％ (1/22)、17.4％ (4/23),17.4％ (4/23)、11.8％ (2/17),Caveolin-1蛋白在基底细胞样型的表达水平强于腔上皮A、腔上皮B型、HER2高表达型和正常乳腺表型(P＜0.01).基底细胞样型caveolin-1蛋白表达与CK5/6、EGFR水平正相关(P＜0.01),与E-cadherin水平无关(P＞0.05).乳腺癌中表达caveolin-1蛋白者淋巴结转移率亦高(69.2％ vs.46.8％,P=0.047).乳腺癌患者中caveolin-1(±)5年无瘤生存率(10/26,38.46％)显著低于caveolin-1(一)患者(59/79,74.68％,P=0.0004).但多因素分析未证明caveolin-1为乳腺癌患者无瘤生存的预后因素(P＞0.05).结论 Caveolin-1可作为基底细胞样乳腺癌的一个筛选标记,可能促进癌细胞的转移,但不是影响乳腺癌患者预后的独立因素.
목적 탐토caveolin-1재유선암중적표체급기의의.방법 채용면역조화이보법,검측105례유선암화50례비암유선조직중caveolin-1적표체정황,동시검측조직중CK5/6、EGFR、E-cadherin표체정황,연구기여eaveolin-1표체적관계,병대105례유선암환자적림상병리자료진행회고성분석.결과 105례유선암안기인분형분위기저세포양유선암20례,강상피A형유선암22례,강상피B형유선암23례,HER2고표체형유선암23례,정상유선표형유선암17례.Caveolin-1단백재유선암화비암유선조직적표체솔분별위24.8％ (26/105),88％ (44/50).Caveolin-1재기저세포양형,강상피A형,강상피B형,HER2고표체형,정상유선표형유선암적표체솔분별위75.0％ (15/20)、4.8％ (1/22)、17.4％ (4/23),17.4％ (4/23)、11.8％ (2/17),Caveolin-1단백재기저세포양형적표체수평강우강상피A、강상피B형、HER2고표체형화정상유선표형(P＜0.01).기저세포양형caveolin-1단백표체여CK5/6、EGFR수평정상관(P＜0.01),여E-cadherin수평무관(P＞0.05).유선암중표체caveolin-1단백자림파결전이솔역고(69.2％ vs.46.8％,P=0.047).유선암환자중caveolin-1(±)5년무류생존솔(10/26,38.46％)현저저우caveolin-1(일)환자(59/79,74.68％,P=0.0004).단다인소분석미증명caveolin-1위유선암환자무류생존적예후인소(P＞0.05).결론 Caveolin-1가작위기저세포양유선암적일개사선표기,가능촉진암세포적전이,단불시영향유선암환자예후적독립인소.
Objective To investigate the expression and significance of caveolin-1 in breast carcinoma.Methods Using immunohistochemical method the protein expression of caveolin-1 were analyzed in 105 cases of breast carcinoma and 50 cases of non-cancerous breast tissues.The relationship between caveolin-1 expression and CK5/6,EGFR,and E-cadherin expression was investigated.Clinical data of 105 cases of breast carcinoma were retrospectively analyzed.Results Among 105 cases of breast carcinoma,there were 20 cases of basal-like subtype,22 cases of luminal subtype A,23 cases of luminal subtype B,23 cases of HER2 over-expressing subtype,17 cases of normal breast-like subtype.Positive rate of caveolin-1 was significantly lower in breast carcinoma than in non-cancerous breast tissues (24.8％ vs.88.0％,P ＜ 0.05).Positive rate of caveolin-1 (75.0％) was higher in breast carcinoma than in luminal subtype A ( 4.8％ ),luminal subtype B ( 17.4％ ),HER2 over-expressing subtype ( 17.4％ ) or normal breast-like subtype( 11.8％ ),all P ＜0.05.Caveolin-1 expression was associated with expression of CK5/6 and EGFR(P ＜0.01 ).In univariate analysis,positive caveolin-1 was associated with higher lymph node metastasis rate (18/26,69.2％ )than negative (37/79,46.8％ ),P =0.047,and shorter 5-year-disease-free survival (38.46％ vs.74.68％,P =0.0004 ),but in multivariate analysis caveolin-1 was not an independent predictor of 5-year- disease-free survival (P ＞ 0.05).Conclusions Caveolin-1 can be seen as a screening mark of basal-like breast carcinoma,it may promote the invasiveness of breast cancer cells,but it is not an independent prognostic predictor of breast cancer patients.