中华口腔医学杂志
中華口腔醫學雜誌
중화구강의학잡지
Chinese Journal of Stomatology
2009年
1期
53-55
,共3页
赵霄虹%祝华珺%鲁大鹏%张敏
趙霄虹%祝華珺%魯大鵬%張敏
조소홍%축화군%로대붕%장민
角蛋白13%维甲酸%三氧化二砷
角蛋白13%維甲痠%三氧化二砷
각단백13%유갑산%삼양화이신
Keratin-13:Tretinoin: Arsenic trioxide
目的 研究细胞角蛋白(cytokeratin,CK)13在口腔鳞状细胞癌中表达的变化规律,探讨全反式维甲酸(all-trans retinoic acid,ATRA)与三氧化二砷(As2O3)在诱导分化人口腔未分化鳞状细胞癌细胞系KB细胞中的作用.方法 采用免疫荧光标记CK-13单克隆抗体及KB细胞细胞核,检测CK-13在ATBA与As2O3诱导分化KB细胞前后的表达变化.将同期培养的KB细胞分为对照组(细胞培养液)、ATRA实验组及As2O3实验组.培养72 h后分别观察3个视野下对照组与实验组KB细胞中CK-13在细胞质中的表达情况,并统计胞质着色率.结果 免疫荧光检测法发现对照组的KB细胞中CK-13绿色荧光染色胞质着色率为(1.00±0.58)%;ATRA实验组的3个浓度组中CK-13绿色荧光染色胞质着色率分别为(100.00±0.00)%、(100.00±0.00)%、(98.96±1.04)%,而As2O3实验组的3个浓度组结果 分别为(100.00±0.00)%、(100.00±0.00)%、(88.64±11.36)%.对照组与实验组CK-13绿色荧光染色胞质着色率的统计数据对比差异均有统计学意义(P＜0.05),各实验组之间相比差异无统计学意义(P＞0.05).结论 ATRA与As2O3对KB细胞均存在诱导分化作用,CK-13定位着色于细胞质,其表达变化与肿瘤细胞分化程度呈正相关,可作为评估ATBA和As2O3对OSCC诱导分化疗效的分子标志物.
目的 研究細胞角蛋白(cytokeratin,CK)13在口腔鱗狀細胞癌中錶達的變化規律,探討全反式維甲痠(all-trans retinoic acid,ATRA)與三氧化二砷(As2O3)在誘導分化人口腔未分化鱗狀細胞癌細胞繫KB細胞中的作用.方法 採用免疫熒光標記CK-13單剋隆抗體及KB細胞細胞覈,檢測CK-13在ATBA與As2O3誘導分化KB細胞前後的錶達變化.將同期培養的KB細胞分為對照組(細胞培養液)、ATRA實驗組及As2O3實驗組.培養72 h後分彆觀察3箇視野下對照組與實驗組KB細胞中CK-13在細胞質中的錶達情況,併統計胞質著色率.結果 免疫熒光檢測法髮現對照組的KB細胞中CK-13綠色熒光染色胞質著色率為(1.00±0.58)%;ATRA實驗組的3箇濃度組中CK-13綠色熒光染色胞質著色率分彆為(100.00±0.00)%、(100.00±0.00)%、(98.96±1.04)%,而As2O3實驗組的3箇濃度組結果 分彆為(100.00±0.00)%、(100.00±0.00)%、(88.64±11.36)%.對照組與實驗組CK-13綠色熒光染色胞質著色率的統計數據對比差異均有統計學意義(P＜0.05),各實驗組之間相比差異無統計學意義(P＞0.05).結論 ATRA與As2O3對KB細胞均存在誘導分化作用,CK-13定位著色于細胞質,其錶達變化與腫瘤細胞分化程度呈正相關,可作為評估ATBA和As2O3對OSCC誘導分化療效的分子標誌物.
목적 연구세포각단백(cytokeratin,CK)13재구강린상세포암중표체적변화규률,탐토전반식유갑산(all-trans retinoic acid,ATRA)여삼양화이신(As2O3)재유도분화인구강미분화린상세포암세포계KB세포중적작용.방법 채용면역형광표기CK-13단극륭항체급KB세포세포핵,검측CK-13재ATBA여As2O3유도분화KB세포전후적표체변화.장동기배양적KB세포분위대조조(세포배양액)、ATRA실험조급As2O3실험조.배양72 h후분별관찰3개시야하대조조여실험조KB세포중CK-13재세포질중적표체정황,병통계포질착색솔.결과 면역형광검측법발현대조조적KB세포중CK-13록색형광염색포질착색솔위(1.00±0.58)%;ATRA실험조적3개농도조중CK-13록색형광염색포질착색솔분별위(100.00±0.00)%、(100.00±0.00)%、(98.96±1.04)%,이As2O3실험조적3개농도조결과 분별위(100.00±0.00)%、(100.00±0.00)%、(88.64±11.36)%.대조조여실험조CK-13록색형광염색포질착색솔적통계수거대비차이균유통계학의의(P＜0.05),각실험조지간상비차이무통계학의의(P＞0.05).결론 ATRA여As2O3대KB세포균존재유도분화작용,CK-13정위착색우세포질,기표체변화여종류세포분화정도정정상관,가작위평고ATBA화As2O3대OSCC유도분화료효적분자표지물.
Objective To examine the expression of cytokeratin-13(CK-13)in oral squamous cell carcinoma(OSCC)and to discuss the effects of all-trans retinoic acid(ATRA)or arsenic trioxide(As2O3)on the differentiation of human oral undifferentiated squamous cell carcinoma cell line KB cells.Methods The cultured KB cells were divided into three groups,ATRA group,As2O3 group,and control The expression of CK-13 in KB cells was detected using the inmmunofluorescence before and after KB cells were induced by ATRA or As2O3.Results The expression rates of CK-13 in KB cells in the ATRA group and As2O3 group were significantly higher than that in the control(P＜0.05),but there was no significant difference in the expression between ATBA and As2O3 group(P＞0.05).Conclusions ATRA and As2O3 both have the ability to differentiate the KB cells,and the expression is associated with the degree of tumor differentiation.CK-13 may serve as a molecular marker to evaluate the effect of the differentiation treatment on OSCC.
