中华微生物学和免疫学杂志
中華微生物學和免疫學雜誌
중화미생물학화면역학잡지
CHINESE JOURNAL OF MICROBIOLOGY AND IMMUNOLOGY
2012年
4期
318-322
,共5页
高勇%宋月%范亚欣%陈玫%肖南%潘凌子%段莹
高勇%宋月%範亞訢%陳玫%肖南%潘凌子%段瑩
고용%송월%범아흔%진매%초남%반릉자%단형
强直性脊柱炎%Th17细胞%调节性T细胞%IL-6%TGF-β
彊直性脊柱炎%Th17細胞%調節性T細胞%IL-6%TGF-β
강직성척주염%Th17세포%조절성T세포%IL-6%TGF-β
Ankylosing spondylitis,Th17 cells%Regulatory T cells%IL-6%TGF-β
目的 探讨强直性脊柱炎( ankylosing spondylitis,AS)患者Th17和CD4+ CD25+ FoxP3+调节性T细胞比例及相关细胞因子水平的变化及意义.方法 强直性脊柱炎患者40例,正常同年龄对照37例.采用流式细胞术检测外周血Th17与调节性T细胞的比例,双抗体夹心酶联免疫吸附法( ELISA)检测血清IL-6、IL-23、IL-17和TGF-β水平.结果 AS组患者外周血Th17细胞比例明显高于对照组[(1.02±0.34)％vs(0.68:±0.29)％,P＜0.05],CD+ CD25+ FoxP3+细胞比例明显低于对照组[(3.77±0.81)％ vs (4.69±1.23)％,P＜0.05].AS患者血清中IL-6、IL-23、IL-17水平明显高于对照组[ (6.15±2.71) ng/L vs(3.31±1.65) ng/L; (9.44±3.12) ng/ml vs (5.82±2.61) ng/ml;( 10.53±4.97) ng/L vs (6.78±3、26) ng/L,P均＜0.01]；差异有统计学意义.与对照组相比,AS组TGF-β水平有下降的趋势[(4,76±2.15) ng/ml vs(5.16±2.02) ng/ml,P＞0.05],但差异无统计学意义.AS患者血清各细胞因子含量与临床及实验室指标无相关性.结论 强直性脊柱炎患者Th17与CD4+CD35+FoxP3+调节性T细胞比例失衡,血清IL-6、IL-23、IL-17和TGF-β水平变化,这些原因可能参与强直性脊柱炎免疫发病过程.
目的 探討彊直性脊柱炎( ankylosing spondylitis,AS)患者Th17和CD4+ CD25+ FoxP3+調節性T細胞比例及相關細胞因子水平的變化及意義.方法 彊直性脊柱炎患者40例,正常同年齡對照37例.採用流式細胞術檢測外週血Th17與調節性T細胞的比例,雙抗體夾心酶聯免疫吸附法( ELISA)檢測血清IL-6、IL-23、IL-17和TGF-β水平.結果 AS組患者外週血Th17細胞比例明顯高于對照組[(1.02±0.34)％vs(0.68:±0.29)％,P＜0.05],CD+ CD25+ FoxP3+細胞比例明顯低于對照組[(3.77±0.81)％ vs (4.69±1.23)％,P＜0.05].AS患者血清中IL-6、IL-23、IL-17水平明顯高于對照組[ (6.15±2.71) ng/L vs(3.31±1.65) ng/L; (9.44±3.12) ng/ml vs (5.82±2.61) ng/ml;( 10.53±4.97) ng/L vs (6.78±3、26) ng/L,P均＜0.01]；差異有統計學意義.與對照組相比,AS組TGF-β水平有下降的趨勢[(4,76±2.15) ng/ml vs(5.16±2.02) ng/ml,P＞0.05],但差異無統計學意義.AS患者血清各細胞因子含量與臨床及實驗室指標無相關性.結論 彊直性脊柱炎患者Th17與CD4+CD35+FoxP3+調節性T細胞比例失衡,血清IL-6、IL-23、IL-17和TGF-β水平變化,這些原因可能參與彊直性脊柱炎免疫髮病過程.
목적 탐토강직성척주염( ankylosing spondylitis,AS)환자Th17화CD4+ CD25+ FoxP3+조절성T세포비례급상관세포인자수평적변화급의의.방법 강직성척주염환자40례,정상동년령대조37례.채용류식세포술검측외주혈Th17여조절성T세포적비례,쌍항체협심매련면역흡부법( ELISA)검측혈청IL-6、IL-23、IL-17화TGF-β수평.결과 AS조환자외주혈Th17세포비례명현고우대조조[(1.02±0.34)％vs(0.68:±0.29)％,P＜0.05],CD+ CD25+ FoxP3+세포비례명현저우대조조[(3.77±0.81)％ vs (4.69±1.23)％,P＜0.05].AS환자혈청중IL-6、IL-23、IL-17수평명현고우대조조[ (6.15±2.71) ng/L vs(3.31±1.65) ng/L; (9.44±3.12) ng/ml vs (5.82±2.61) ng/ml;( 10.53±4.97) ng/L vs (6.78±3、26) ng/L,P균＜0.01]；차이유통계학의의.여대조조상비,AS조TGF-β수평유하강적추세[(4,76±2.15) ng/ml vs(5.16±2.02) ng/ml,P＞0.05],단차이무통계학의의.AS환자혈청각세포인자함량여림상급실험실지표무상관성.결론 강직성척주염환자Th17여CD4+CD35+FoxP3+조절성T세포비례실형,혈청IL-6、IL-23、IL-17화TGF-β수평변화,저사원인가능삼여강직성척주염면역발병과정.
Objective To investigate the percentages of Th17 and CD4+CD25+FoxP3+ regulatory T(Tr) cells and the levels of related cytokines IL-6,IL-23,IL-17 and TGF-β in serum of patients with anlylosing spondylitis(AS).Methods Forty patients with AS and 37 age-matched healthy donors were studied.Flow cytometry Was used to analyze the percentages of blood Th17 and CD4+CD25+FoxP3+Tr cells.The levels of serum IL-6,IL-23,IL-17 and TGF-β were assayed by enzyme-linked immunosorbent assay ( ELISA).Results The proportion of Th17 cells in AS group was significantly higher than those in normal group [ (1.02±0.34)％ vs (0.68±0.29)％,P＜0.05) ],and the proportion of CD4+CD25+FoxP3+ cells was lower in AS group comparing with normal group [(3.77±0.81)％ vs (4.69±1.23)％,P＜0.05)].Meanwhile,serum levels of IL-6,IL-23 and IL-17 were significantly higher in AS group than those in normal group [ (6,15±2.71) ng/L vs (3.31±1.65) ng/L; (9.44±3.12) ng/ml vs (5.82±2.61) ng/ml;(10.53±4.97) ng/L vs (6.78±3.26) ng/L,all P＜0.01 ].In contrast,TGF-β level was decreased in AS group compares with the normal group [ ( 4.76±2.15) ng/ml vs (5.16±2.02) ng/ml,P＞0.05 ],but the difference was not significant.No associations of serum eytokine levels with clinical and laboratory parameters were found in AS.Conclusion The abnormality Th17 cells and Tr cells and their related cytokines IL-6,IL-23,IL-17 and TGF-β changes in patients with AS,which may be involved in immunological pathogenesis of AS.
