CAJ | 학술논문

目的观察人肺腺癌细胞调节性细胞容积减小(RVD)的过程,探讨其离子通道机制.方法用细胞体积测量系统分析人肺腺癌细胞(A549)在细胞外低渗刺激下细胞容积改变;用通道阻断剂和全细胞膜片钳方法阐明容积敏感性氯通道在RVD中的作用.结果细胞外低渗刺激使人肺腺癌细胞系A549细胞膨胀并诱发RVD,该RVD过程可被氯通道阻断剂NPPB(100 μmol/L)和钾通道阻断剂CsCl(5 mmoL/L)所阻抑;全细胞膜片钳方法记录A549细胞容积敏感性氯通道电流呈外向整流特性,电流可被NPPB、DIDS(100 μmol/L)阻抑.结论人肺腺癌A549细胞具有RVD功能,RVD过程的完成有赖于氯通道和钾通道的平行激活,容积敏感性氯通道参与该RVD过程.
목적 관찰인폐선암세포조절성세포용적감소(RVD)적과정,탐토기리자통도궤제.방법 용세포체적측량계통분석인폐선암세포(A549)재세포외저삼자격하세포용적개변;용통도조단제화전세포막편겸방법 천명용적민감성록통도재RVD중적작용.결과 세포외저삼자격사인폐선암세포계A549세포팽창병유발RVD,해RVD과정가피록통도조단제NPPB(100 μmol/L)화갑통도조단제CsCl(5 mmoL/L)소조억;전세포막편겸방법 기록A549세포용적민감성록통도전류정외향정류특성,전류가피NPPB、DIDS(100 μmol/L)조억.결론 인폐선암A549세포구유RVD공능,RVD과정적완성유뢰우록통도화갑통도적평행격활,용적민감성록통도삼여해RVD과정.
Objective This study was designed to observe the regulatory volume decrease (RVD) process in human lung adenocarcinoma cells (A549) and to investigate its ion channel mechanism.Methods Electric measurement system of cell volume was used to detect the cell volume changes following exposure to hypotenic solution.Whole-cell patch clamp recordings were applied to investigate the characteristics of the volume-sensitive Cl~- channel in A549 cells.Results Extracelluar hypotenicity induced cell swelling followed by a typical RVD process,which can be inhibited by Cl~- channel blocker (NPPB 100 μmoL/L) and K~+ channel blocker (CsCl 5 mmol/L).Meanwhile,a outward-rectifying chloride currents which was sensitive to NPPB and DIDs was recorded in A.549 using the whole cell patch clamp.Conclusions The human lung adenacarcinoma cells has RVD process which is dependent on the parallel activation of Cl~- channel and K~+ channel.The volume-sensitive Cl~- channel is involved in volume regulation of lung adenocarcinoma cells.