中华医学遗传学杂志
中華醫學遺傳學雜誌
중화의학유전학잡지
CHINESE JOURNAL OF MEDICAL GENETICS
2012年
3期
306-308
,共3页
王志林%戴莉%李桑%邱国强%吴浩清
王誌林%戴莉%李桑%邱國彊%吳浩清
왕지림%대리%리상%구국강%오호청
慢性髓细胞白血病%短串联重复序列%基因频率
慢性髓細胞白血病%短串聯重複序列%基因頻率
만성수세포백혈병%단천련중복서렬%기인빈솔
Chronic myeloid leukemia%Short tandem repeat%Gene frequency
目的 比较15个短串联重复序列(short tandem repeats,STR)基因座(D8S1179、D21Sll、D7S820、CSFl PO、D3S1358、TH01、D13S317、D16S539、D2S1338、D19S433、vWA、TPOX、D18S51、D5S818、FGA)在慢性髓细胞白血病(chronic myeloid leukemia,CML)患者和常州地区正常人群中的频率,并预测与CML的相关基因.方法 收集745份常州地区正常人以及132份CML缓解期患者的外周血样.用PCR荧光标记复合扩增系统对样本DNA进行基因型分析.比较两组人群的基因频率,并根据15个基因座等位基因频率的差异预测与CML相关的基因.结果 CML患者CSFlPO、vWA、TPOX基因座的等位基因型分布与正常人相比差异有统计学意义(P＜0.05),其余12个基因座等位基因的分布差异无统计学意义(P＞0.05).结论CSFlPO、vWA、TPOX基因座与CML的发生相关,但其生物学意义尚不清楚.
目的 比較15箇短串聯重複序列(short tandem repeats,STR)基因座(D8S1179、D21Sll、D7S820、CSFl PO、D3S1358、TH01、D13S317、D16S539、D2S1338、D19S433、vWA、TPOX、D18S51、D5S818、FGA)在慢性髓細胞白血病(chronic myeloid leukemia,CML)患者和常州地區正常人群中的頻率,併預測與CML的相關基因.方法 收集745份常州地區正常人以及132份CML緩解期患者的外週血樣.用PCR熒光標記複閤擴增繫統對樣本DNA進行基因型分析.比較兩組人群的基因頻率,併根據15箇基因座等位基因頻率的差異預測與CML相關的基因.結果 CML患者CSFlPO、vWA、TPOX基因座的等位基因型分佈與正常人相比差異有統計學意義(P＜0.05),其餘12箇基因座等位基因的分佈差異無統計學意義(P＞0.05).結論CSFlPO、vWA、TPOX基因座與CML的髮生相關,但其生物學意義尚不清楚.
목적 비교15개단천련중복서렬(short tandem repeats,STR)기인좌(D8S1179、D21Sll、D7S820、CSFl PO、D3S1358、TH01、D13S317、D16S539、D2S1338、D19S433、vWA、TPOX、D18S51、D5S818、FGA)재만성수세포백혈병(chronic myeloid leukemia,CML)환자화상주지구정상인군중적빈솔,병예측여CML적상관기인.방법 수집745빈상주지구정상인이급132빈CML완해기환자적외주혈양.용PCR형광표기복합확증계통대양본DNA진행기인형분석.비교량조인군적기인빈솔,병근거15개기인좌등위기인빈솔적차이예측여CML상관적기인.결과 CML환자CSFlPO、vWA、TPOX기인좌적등위기인형분포여정상인상비차이유통계학의의(P＜0.05),기여12개기인좌등위기인적분포차이무통계학의의(P＞0.05).결론CSFlPO、vWA、TPOX기인좌여CML적발생상관,단기생물학의의상불청초.
[Objective]To compare allelic frequencies of 15 short tandem repeat(STR)loci (D8Sl179,D21S11,D7S820,CSF1PO,D3S1358,TH01,D13S317,D16S539,D2S1338,D19S433,vWA,TPOX,D18S51,D5S818 and FGA) between chronic myeloid leukemia (CML) patients and non-related healthy individuals from Changzhou region in order to predict genes related with the CML.[Methods] Blood samples were collected from 745 healthy subjects and 132 CML patients with complete remission.Genotypes were determined with gene scan technology and multiplex PCR with fluorescence-labeled primers.Allelic polymorphisms of 15 STR loci were compared between the two groups.Potential genes related with CML were predicted with statistical analysis of differences in allelic frequencies.[Results] Allelic frequencies of 3 loci,including CSF1PO,vWA and TPOX,showed a significant difference (P＜ 0.05) between the two groups.[Conclusion] CSF1PO,vWA and TPOX loci may be related with CML,albeit that the exact biologic mechanisms is unclear.
