CAJ | 학술논문

目的研究梭曼染毒大鼠丙二醛（MDA）含量和单胺氧化酶（MAO）活性的变化，探讨抗氧化剂VA，VE 对梭曼染毒大鼠自由基损伤的保护作用. 方法雄性大鼠 40只，按体质量随机分为4组：阴性对照组，阳性对照组，VA和VE实验组. 观察染毒大鼠血清、大脑、肝、肾组织MDA 含量和MAO活性的变化. 结果梭曼(GD)染毒大鼠阳性对照组5阴性5对照组相比较，脑组织MDA含量和MAO活性有显著性增加（P<0.05）；血清蛋白有显著减少（P<0.05），肝蛋白有显著增加（ P<0.05）. VA实验组与阳性对照组比较，脑组织MDA有显著增加（P<0.01），肝组织MDA有显著降低（P< 0.01）；脑组织MAO活性增加，但差异不显著. VE实验组与阳性对照组相比，肾脏MDA有显著减少（P<0.0 1）；脑组织MAO活性有非常显著的降低（P<0.01）. 施加VA, VE 抗氧化剂后，与阳性对照组比较，血清和肝蛋白有非常显著减少（P<0.01），脑蛋白有显著减少（P<0.05）. 结论梭曼(GD)染毒引起明显脂质过氧化损伤，VE具有抗氧化保护作用，而VA有协同促进MDA升高的过氧化损伤作用.
목적연구사만염독대서병이철（MDA）함량화단알양화매（MAO）활성적변화，탐토항양화제VA，VE 대사만염독대서자유기손상적보호작용. 방법웅성대서 40지，안체질량수궤분위4조：음성대조조，양성대조조，VA화VE실험조. 관찰염독대서혈청、대뇌、간、신조직MDA 함량화MAO활성적변화. 결과사만(GD)염독대서양성대조조5음성5대조조상비교，뇌조직MDA함량화MAO활성유현저성증가（P<0.05）；혈청단백유현저감소（P<0.05），간단백유현저증가（ P<0.05）. VA실험조여양성대조조비교，뇌조직MDA유현저증가（P<0.01），간조직MDA유현저강저（P< 0.01）；뇌조직MAO활성증가，단차이불현저. VE실험조여양성대조조상비，신장MDA유현저감소（P<0.0 1）；뇌조직MAO활성유비상현저적강저（P<0.01）. 시가VA, VE 항양화제후，여양성대조조비교，혈청화간단백유비상현저감소（P<0.01），뇌단백유현저감소（P<0.05）. 결론사만(GD)염독인기명현지질과양화손상，VE구유항양화보호작용，이VA유협동촉진MDA승고적과양화손상작용.
AIM　To study the changes of malondialdehyde(MDA) content and monoamine (MAO) activity in rat injured by Soman (GD), and the antioxidation effect of vitamin A (VA) and vitamin E (VE). METHODS　Forty male rats were rand omly div ided into 4 groups: negative group, positive group, VA and VE trail group. A fter normally fed for 10 d, GD is injected into muscle of rates of positive contr ol group and VA, VE trail group. After toxic symptoms occu rred in rate of positive group and VA, VE trail group partly for 2 h, th e rats were kille d by dislocation of head. MDA content and MAO activity of rat's serum, cerebrum, liver, kidney we re determined respectively. RESULTS　compared with negative control group, con tent of MDA and activity of MAO increase significantly in cerebrum (P<0.05), concentration of serum prot ein increase and that of live protein decrease obviously (P<0.05) in positive contr ol group; in VA trial, content of MDA in cerebrum increase obviously (P<0.01), but that of MDA in kidney decrease apparently (P<0.01), activity of MAO have rarely increase. in VE trial, c ontent of MDA in kidney decrease apparently (P<0.01), and activity of MAO reduce signific antly (P<0.01); in VA and VE trial, concentration of serum and live protein decrease remarkabl y (P< 0.01), that of cerebrum protein lessen apparently (P<0.05). CONCLUSION　GD raised lipid superoxidation, and VE decease obviously free radical injury,but VA accelerate the injury of free ra dical intoxicated rat.